Small-animal SPECT and SPECT/CT: application in cardiovascular research

Golestani, Reza; Wu, Chao; Tio, René A.; Zeebregts, Clark J.; Petrov, Artiom; Beekman, Freek J.; Dierckx, Rudi; Boersma, Hendrikus H.; Slart, Riemer H. J. A.

doi:10.1007/s00259-009-1321-8

Cited by 45 publications

(49 citation statements)

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“…This is likely due to the typically lower spatial resolution of older-generation microSPECT systems. Recently, sub-half millimeter resolution has been achieved with a new generation of microSPECT scanners using multi-pinhole collimation [20]. To date, no studies have evaluated high-resolution microSPECT for cardiac functional imaging in the mouse, or compared microSPECT with other modalities for use in preclinical cardiac imaging.…”

Section: Introductionmentioning

confidence: 99%

Comparison of 4D-MicroSPECT and MicroCT for Murine Cardiac Function

2013

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Purpose The objective of this study was to compare a new generation of four-dimensional (4D) microSPECT with microCT for quantitative in vivo assessment of murine cardiac function. Procedures 4D isotropic cardiac images were acquired from normal C57BL/6 mice with either microSPECT at 350-micron resolution (n=6) or microCT at 88-micron resolution (n=6). One additional mouse with myocardial infarction (MI) was scanned with both modalities. Prior to imaging, mice were injected with either 99mTc -tetrofosmin for microSPECT, or a liposomal blood pool contrast agent for microCT. Segmentation of the left ventricle (LV) was performed using Vitrea (Vital Images) software, to derive global and regional function. Results Measures of global LV function between microSPECT and microCT groups were comparable (e.g. ejection fraction=71±6%-microSPECT and 68±4%-microCT). Regional functional indices (wall motion, wall thickening, regional ejection fraction) were also similar for the two modalities. In the mouse with MI, microSPECT identified a large perfusion defect that was not evident with microCT. Conclusions Despite lower spatial resolution, microSPECT was comparable to microCT in the quantitative evaluation of cardiac function. MicroSPECT offers an advantage over microCT in the ability to evaluate myocardial perfusion radiotracer distribution and function simultaneously. MicroSPECT should be considered as an alternative to microCT and MR for preclinical cardiac imaging in the mouse.

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Section: Introductionmentioning

confidence: 99%

Comparison of 4D-MicroSPECT and MicroCT for Murine Cardiac Function

2013

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“…Prospective or retrospective single (ECG) or dual (ECG and respiration) gating strategies have been therefore implemented to improve mouse cardiac image quality. Depending on the imaging method and the mouse heart rate (around 500 beats/min under anesthesia), temporal resolutions that are now obtained are in the range of 5 to 15 msec, while spatial resolutions are between 100 × 100 × 100 µm 3 and 1.5 × 1.5 ×1.5 mm 3 (Badea et al, ; Kreissl et al, ; Feintuch et al, ; Bucholz et al, ; Golestani et al, ). Based on our experience, the throughput of each imaging method is around 10 animals a day for a typical protocol.…”

Section: : Introduction: a Short Historical Perspective On In Vivo Mmentioning

confidence: 99%

PET, SPECT, CT, and MRI in Mouse Cardiac Phenotyping: An Overview

et al. 2012

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This overview first summarizes the last decade of continuous developments and improvements in pre-clinical imaging methods that are now essential tools for in vivo evaluation of cardiac morphology and function in living mice, involving nuclear emission of labeled molecules (micro-PET and micro-SPECT) and electromagnetic wave interactions with biological tissues (micro-CT and micro-MRI). In the following, and for better understanding, the basic physical principles and specific technical innovations of the aforementioned imaging methods are reviewed. Specificity, sensitivity, and spatial and temporal resolutions, together with the corresponding advantages and weaknesses of each method are then discussed, and cardiac image-acquisition protocols and illustrative examples are given for each modality. Emerging hybrid cardiac imaging is also presented and illustrated. Then, recent biological insights provided by mouse cardiac imaging are presented. Finally, imaging strategies in mouse cardiac phenotyping involving the aforementioned methods, adding metabolic and molecular information to morphological data, are emphasized and discussed. Curr. Protoc. Mouse Biol. 2:129-144 © 2012 by John Wiley & Sons, Inc.

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“…Although x-ray based CT is not optimal for small animal imaging this method facilitates the localization of labeleld molecules in biodistribution studies. Today there are few manufacturers, which provide combined SPECT/CT and PET/CT equipments for clinical use but also dedicated animal devices are on the market (Picler et al 2008, Golestani et al 2010. The advantage of these multimodality systems is the ease of imaging with different modalities without moving the object and hence the co-localization of images can be performed easily (Figure 2).…”

Section: Ctmentioning

confidence: 99%