Small animal models of thermal injury

Vinaik, Roohi; Aijaz, Ayesha; Jeschke, Marc G.

doi:10.1016/bs.mcb.2021.12.014

Cited by 7 publications

(3 citation statements)

References 155 publications

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“…Burn injury is a powerful pathological stimulus of beige fat biogenesis and a hypermetabolic state; 6 , 24 however, the developmental process remains poorly characterized. Following the established protocol in mice, 25 we harvested inguinal WAT depots of C57BL/6J mice at 24 h (1 day), 3 days, and 7 days after burn injury or sham ( Figure 2A ). Plasma free fatty acid (FFA) levels were significantly elevated at 3 and 7 days after burn injury compared with sham control mice ( Figure 2B ).…”

Section: Resultsmentioning

confidence: 99%

Lipolysis-derived linoleic acid drives beige fat progenitor cell proliferation

Abe¹,

Oguri²,

Verkerke³

et al. 2022

Developmental Cell

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Section: Resultsmentioning

confidence: 99%

Lipolysis-derived linoleic acid drives beige fat progenitor cell proliferation

Abe¹,

Oguri²,

Verkerke³

et al. 2022

Developmental Cell

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“…A total of 24 animals (BURNS, BURNS+HBO, BURNS+FU, and BURNS+HBO+FU) were weighed and anesthetized with the combination of ketamine and xylazine (10 and 5 mg/kg, i.p., respectively). Antisepsis was achieved by local application of 1% polyvinylpyrrolidone iodine before trichotomy of a back area measuring approximately 3 cm 2 while the thermal skin injuries were induced using the electrically driven solid aluminum bar (10 mm in diameter), to achieve the constant temperature of 75 °C for 15 s on the preferred (dorsal proximal thoracic) region [ 26 , 54 ], following the exact previously described procedure taking into account clinical and histological characteristics [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and Filipendula ulmaria Extract in Rats

Krstic,

Selakovic,

Jovicic

et al. 2024

IJMS

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The aim of this study was to evaluate the alterations of the hippocampal function that may be related to anxiogenic response to thermal skin injury, including the morpho-functional alterations, and the effects of hyperbaric oxygen (HBO) and Filipendula ulmaria (FU) extract in the treatment of anxiety-like behavior that coincides with thermal skin injury. A rat thermal skin injury experimental model was performed on 2-month-old male Wistar albino rats. The evaluated therapeutic protocols included HBO and/or antioxidant supplementation. HBO was applied for 7 days in the hyperbaric chamber (100% O2, 2.5 ATA, 60 min). Oral administration of FU extract (final concentration of 100 mg/kg b.w.) to achieve antioxidant supplementation was also applied for 7 days. Anxiety level was estimated in the open field and elevated plus-maze test, which was followed by anesthesia, sacrifice, and collection of hippocampal tissue samples. HBO treatment and FU supplementation significantly abolished anxiogenic response to thermal skin injury. This beneficial effect was accompanied by the reduction in hippocampal pro-inflammatory and pro-apoptotic indicators, and enhanced BDNF and GABA-ARα2S gene expression, previously observed in untreated burns. The hippocampal relative gene expression of melatonin receptors and NPY positively responded to the applied protocols, in the same manner as µ and δ opioid receptors, while the opposite response was observed for κ receptors. The results of this study provide some confirmations that adjuvant strategies, such as HBO and antioxidant supplementation, may be simultaneously applied in the treatment of the anxiety-like behavior that coincides with thermal skin injury.

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“…), before trichotomy of a back area measuring approximately 3 cm 2 (1% polyvinylpyrrolidone iodine was locally applied as antisepsis). Thermal injuries were made with a solid aluminium bar (10 mm in diameter) that was electrically driven to maintain the constant temperature of 75 °C [ 26 ]. The bar was maintained in contact with the animal skin on the dorsal proximal region for 15 s.…”

Section: Methodsmentioning

confidence: 99%

Simultaneous Administration of Hyperbaric Oxygen Therapy and Antioxidant Supplementation with Filipendula ulmaria Extract in the Treatment of Thermal Skin Injuries Alters Nociceptive Signalling and Wound Healing

Krstic,

Jovicic,

Selakovic

et al. 2023

Medicina

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Background and Objectives: Thermal skin injuries are a prevalent cause of skin damage, potentially leading to severe morbidity and significant mortality. In this study, we intended to estimate the effects of HBO (hyperbaric oxygen treatment) and antioxidant supplementation with Filipendula ulmaria extract, individually and simultaneously, in the treatment of thermal skin injuries. Materials and Methods: As a thermal skin injury experimental model, we used two-month-old male Wistar albino rats. Thermal injuries were made with a solid aluminium bar at a constant temperature of 75 °C for 15 s. Hyperbaric oxygen treatment was performed in a specially constructed hyperbaric chamber for rats (HYB-C 300) for seven consecutive days (100% O2 at 2.5 ATA for 60 min). Antioxidant supplementation was performed with oral administration of Filipendula ulmaria extract dissolved in tap water to reach a final concentration of 100 mg/kg b.w. for seven consecutive days. Results: Simultaneous administration of hyperbaric oxygen therapy and antioxidant supplementation with Filipendula ulmaria extract significantly ameliorated the macroscopic and histopathological characteristics of the wound area and healing. Also, this therapeutic approach decreased the local expression of genes for proinflammatory mediators and increased the expression of the μ-opioid receptor and the MT1 and MT2 receptors in the wound area and spinal cord, with a consequent increase in reaction times in behavioural testing. Conclusions: In conclusion, the presented results of our study allow evidence for the advantages of the simultaneous employment of HBO and antioxidant supplementation in the treatment of thermal skin injuries, with special reference to the attenuation of painful sensations accompanied by this type of trauma.

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Small animal models of thermal injury

Cited by 7 publications

References 155 publications

Lipolysis-derived linoleic acid drives beige fat progenitor cell proliferation

Lipolysis-derived linoleic acid drives beige fat progenitor cell proliferation

Complex Hippocampal Response to Thermal Skin Injury and Protocols with Hyperbaric Oxygen Therapy and Filipendula ulmaria Extract in Rats

Simultaneous Administration of Hyperbaric Oxygen Therapy and Antioxidant Supplementation with Filipendula ulmaria Extract in the Treatment of Thermal Skin Injuries Alters Nociceptive Signalling and Wound Healing

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