Smad4 is essential for directional progression from committed neural progenitor cells through neuronal differentiation in the postnatal mouse brain

Kawaguchi-Niida, Motoko; Shibata, Noriyuki; Furuta, Yasuhide

doi:10.1016/j.mcn.2017.06.008

Cited by 7 publications

(5 citation statements)

References 42 publications

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“…Therefore, BMPpromoted cell survival might be affected (Grotewold and Rüther, 2002;Sharov et al, 2003). In addition, Smad4, the commonmediator Smad (ten Dijke and Heldin, 2006), is a key regulator of the directional progression of postnatal V-SVZ NSCs towards the neuronal lineage (Colak et al, 2008;Kawaguchi-Niida et al, 2017). Conditional Smad4 deletion in V-SVZ progenitors results in an increase of NSC-like properties and differentiation defects (Kawaguchi-Niida et al, 2017).…”

Section: Zeb2 Protein Domains Control Distinct Aspects Of Ob Interneumentioning

confidence: 99%

“…In addition, Smad4, the commonmediator Smad (ten Dijke and Heldin, 2006), is a key regulator of the directional progression of postnatal V-SVZ NSCs towards the neuronal lineage (Colak et al, 2008;Kawaguchi-Niida et al, 2017). Conditional Smad4 deletion in V-SVZ progenitors results in an increase of NSC-like properties and differentiation defects (Kawaguchi-Niida et al, 2017). We propose that the introduction of Zeb2 SBD in a Zeb2-KO background renders the cells incompetent to correctly regulate Smad family signaling, potentially leaving the cells for a longer period in the proliferative phase preceding their differentiation into OB interneurons and preventing apoptosis.…”

Section: Zeb2 Protein Domains Control Distinct Aspects Of Ob Interneumentioning

confidence: 99%

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Multifaceted actions of Zeb2 in postnatal neurogenesis from the ventricular-subventricular zone to the olfactory bulb

et al. 2020

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The transcription factor Zeb2 controls fate specification and subsequent differentiation and maturation of multiple cell types in various embryonic tissues. It binds many protein partners, including activated Smad proteins and the NuRD co-repressor complex. How Zeb2 subdomains support cell differentiation in various contexts has remained elusive. Here, we studied the role of Zeb2 and its domains in neurogenesis and neural differentiation in the young postnatal ventricular-subventricular zone (V-SVZ), in which neural stem cells generate olfactory bulb-destined interneurons. Conditional Zeb2 knockouts and separate acute loss-and gain-of-function approaches indicated that Zeb2 is essential for controlling apoptosis and neuronal differentiation of V-SVZ progenitors before and after birth, and we identified Sox6 as a potential downstream target gene of Zeb2. Zeb2 genetic inactivation impaired the differentiation potential of the V-SVZ niche in a cell-autonomous fashion. We also provide evidence that its normal function in the V-SVZ also involves non-autonomous mechanisms. Additionally, we demonstrate distinct roles for Zeb2 protein-binding domains, suggesting that Zeb2 partners co-determine neuronal output from the mouse V-SVZ in both quantitative and qualitative ways in early postnatal life.

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Section: Zeb2 Protein Domains Control Distinct Aspects Of Ob Interneumentioning

confidence: 99%

Section: Zeb2 Protein Domains Control Distinct Aspects Of Ob Interneumentioning

confidence: 99%

Multifaceted actions of Zeb2 in postnatal neurogenesis from the ventricular-subventricular zone to the olfactory bulb

et al. 2020

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“…Hsa-miR-1260b tended to be expressed more frequently in POAG patients. This miRNA was previously shown to target genes regulating proliferation and differentiation of neuronal cells, e.g., LMX1B [ 35 ], SMAD4 [ 36 ], WNK1 [ 37 ], and CREB1 [ 38 ]. Genetic variants and mutations in LMX1B were previously associated with susceptibility of glaucoma [ 39 – 42 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNAs in the aqueous humor of patients with different types of glaucoma

Kosior‐Jarecka

Czop

Gasińska

et al. 2021

Graefes Arch Clin Exp Ophthalmol

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Purpose The aim of the study was to compare the frequency and the level of expression of selected miRNAs in the aqueous humor of patients with various types of glaucoma. Methods The studied group consisted of 42 patients with glaucoma: 19 with primary open-angle glaucoma (POAG), 14 with pseudoexfoliation glaucoma (PEXG), 9 with primary angle closure glaucoma (PACG), and the control group of 36 patients with senile cataract without glaucoma. The real-time polymerase chain reaction method was used to analyze the expression of miRNAs. Results There were no significant differences in the frequency and the level of miRNA expression between various types of glaucoma. There was a tendency for hsa-miR-6722-3p and hsa-miR-184 to be expressed more frequently in PEXG and hsa-miR-1260b in POAG. The expression levels of hsa-miR-1260b and hsa-miR-6515-3p were correlated with age in POAG. Target annotation and functional analyses showed that genes targeted by the most frequently expressed miRNAs (hsa-miR-1202, -1260b, -184, -187-5p, -6515-3p, -6722-3p, and hsa-mir-4634) are involved mainly in response to hypoxia, cardiovascular system development, and apoptosis. Conclusion Hsa-miR-1260b was the most abundantly expressed among studied miRNAs and may be a potential biomarker of clinical status in PEXG and PACG.

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“…Related morphan receptor A (RORA) is usually found in mesenchymal stem cells originating from bone marrow, and its function in the oocyte could be regulating its development [48]. Kawaguchi-Niida et al have shown that, for the TGFβ/BMP signaling pathway, expression of SMAD4 is needed to ensure differentiated neural progenitor cells don't reverse their transition [49]. The presence of SMAD4 was also studied by Xing et al, and in particular they found it mainly expressed in the oocyte, but also in its surrounding granulosa and theca cells, whilst its expressing being dependent on the stage of follicle development [50].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro – an oocyte model approach

Moncrieff

Kocherova

Bryja

et al. 2020

Medical Journal of Cell Biology

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The growth and development of the oocyte is essential for the ovarian follicle. Cumulus cells (CCs) - a population of granulosa cells - exchange metabolites, proteins and oocyte-derived paracrine factors with the oocyte through gap junctions, to contribute to the competency and health of the oocyte. This bi-directional communication of the cumulus-oocyte complex could be better understood through the micro-analysis of a porcine oocyte gene expression before in vitro maturation (IVM) and after. Additionally, the study of the somatic and gamete cells differentiation capability into neuronal lineage would be promising for future stem cell research as granulosa cells are easily accessible waste material from in vitro fertilization (IVF) procedures. Therefore, in this study, the oocytes of 45 pubertal Landrace gilts were isolated and the protein expression of the COCs were analyzed through micro-analysis techniques. Genes belonging to two ontological groups: neuron differentiation and negative regulation of cell differentiation have been identified which have roles in proliferation, migration and differentiation. Twenty identified porcine oocyte genes (VEGFA, BTG2, MCOLN3, EGR2, TGFBR3, GJA1, FST, CTNNA2, RTN4, MDGA1, KIT, RYK, NOTCH2, RORA, SMAD4, ITGB1, SEMA5A, SMARCA1, WWTR1 and APP) were found to be down-regulated after the transition of IVM compared to in vitro. These results could be applied as gene markers for the proliferation, migration and differentiation occurring in the bi-directional communication between the oocyte and CCs.Running title: Differentiation and neurogenesis in oocyte cells

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Smad4 is essential for directional progression from committed neural progenitor cells through neuronal differentiation in the postnatal mouse brain

Cited by 7 publications

References 42 publications

Multifaceted actions of Zeb2 in postnatal neurogenesis from the ventricular-subventricular zone to the olfactory bulb

Multifaceted actions of Zeb2 in postnatal neurogenesis from the ventricular-subventricular zone to the olfactory bulb

MicroRNAs in the aqueous humor of patients with different types of glaucoma

Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro – an oocyte model approach

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