Smad4-dependent regulation of type I collagen expression in the muscle of grass carp fed with faba bean

Yu, Ermeng; Ma, Lingling; Ji, Hong; Li, Zhifei; Wang, Guangjun; Xie, Jun; Yu, Deguang; Kaneko, Gen; Tian, Jingjing; Zhang, Kai; Gong, Wangbao

doi:10.1016/j.gene.2018.10.074

Cited by 48 publications

(30 citation statements)

References 45 publications

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“…As the TGF-β1/Smads signalling pathway, it should be noted that TGF-β1 bound to the TGF-β type II and type I receptor in turn, and the activated TGF-β type I receptor interacted with Smad2 to phosphorylate it, and then, phosphorylated Smad2 bound to Smad4 forming the Smad2/4 complex, and finally, the complex was transferred to the nuclear to work (73) . In addition, previous studies showed that Smad2 and Smad4 played important regulatory role in type I collagen expression in mammals and grass carp (15,(74)(75)(76) . For the first time, our present results for on-growing grass carp displayed that in comparison with control group, optimal level of Met significantly elevated the mRNA abundances of TGF-β1, Smad4, Smad2 and the protein levels of Smad4, t-Smad2 and p-Smad2 Ser467 as well as the ratio of p-Smad2 Ser467 : t-Smad2 in the muscle.…”

Section: Methionine Promoted Muscle Fibre Hypertrophy and Type I Collagen Synthesis In Fishmentioning

confidence: 96%

“…Similar observation could be found in the study of tryptophan for on-growing grass carp (26) . Furthermore, recent research has displayed that type I collagen expression in the muscle could be modulated by TGF-β1/Smads signaling pathway in grass carp (15) . Therefore, we next explored the impact of methionine on TGF-β1/Smads in the muscle of on-growing grass carp.…”

Section: Methionine Promoted Muscle Fibre Hypertrophy and Type I Collmentioning

confidence: 99%

“…Besides, collagen is the major component of intramuscular connective tissue influencing the functional and structural properties of muscle (12) , the synthesis of which inhibits the differentiation of myogenesis of C2C12 muscle cells in vitro. (13) In grass carp, the main collagen in intramuscular connective tissue is type I collagen (14) , the expression of which is regulated by the transforming growth factor-beta1 (TGF-β1)/Smads signaling pathway at the transcriptional level (15) . Type I collagen consists of α1 and α2 peptide chains (called collagen type 1 alpha 1 (Col1α1) and collagen type 1 alpha 2 (Col1α2)), respectively (14) .…”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary methionine on growth performance, muscle nutritive deposition, muscle fibre growth and type I collagen synthesis of on-growing grass carp (Ctenopharyngodon idella)

et al. 2020

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In the current research, a 60-day experiment was conducted with the purpose of exploring the impacts of methionine on growth performance, muscle nutritive deposition, muscle fibre growth and type I collagen synthesis as well as related signaling pathway. Six diets (iso-nitrogenous) differing in methionine concentrations (2.54, 4.85, 7.43, 10.12, 12.40 and 15.11 g kg-1 diets) were fed to 540 grass carp (178.47 ± 0.36 g). Results showed (P < 0.05) that, compared with methionine deficiency, optimal level of dietary methionine (1) increased feed intake (FI), feed efficiency (FE), specific growth rate (SGR) and percentage weight gain (PWG); (2) increased fish muscle protein, lipid and free amino acid contents, and improved fish muscle fatty acid profile as well as increased protein content in part associated with TORC1/S6K1 signaling pathway; (3) increased the frequency distribution of muscle fibre with >50 µm of diameter; (4) increased type I collagen synthesis partly related to TGF-β1/Smads and CK2/TORC1 signaling pathways. In conclusion, dietary methionine improved muscle growth, which might be due to the regulation of muscle nutritive deposition, and muscle fibre growth and type I collagen synthesis related signal molecules. Finally, according to PWG and muscle collagen content, the methionine requirements for on-growing grass carp (178-626 g) were estimated to be 9.56 g kg-1 diet (33.26 g kg-1 protein of diet) and 9.28 g kg-1 diet (32.29 g kg-1 of dietary protein), respectively.

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Section: Methionine Promoted Muscle Fibre Hypertrophy and Type I Collagen Synthesis In Fishmentioning

confidence: 96%

Section: Methionine Promoted Muscle Fibre Hypertrophy and Type I Collmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of dietary methionine on growth performance, muscle nutritive deposition, muscle fibre growth and type I collagen synthesis of on-growing grass carp (Ctenopharyngodon idella)

et al. 2020

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“…An accidental discovery was made in the early 1970s in Dongsheng Town, Guangdong Province, China, that feeding crisp grass carp ( Ctenopharyngodon idellus C.et V) that had reached a certain level of maturity faba beans led to carp that exhibited more hardness and crispiness in its muscles than ordinary grass carp, and this diet has since become popular. The crisp mechanism of grass carp may be due to a certain factor in faba beans that activates the TGF-β/Smads signaling pathway to regulate type I collagen in muscles, which is the main type of collagen and is closely related to changes in muscle properties [1,2,3]. Furthermore, a proteomic profile demonstrated that muscle fiber hyperplasia was closely related to a protein-protein network of 12 muscle component proteins, and the abundance of the fatty acid degradation and calcium signaling pathways was reduced.…”

Section: Introductionmentioning

confidence: 99%

Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study

et al. 2019

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Many reports of the intestinal microbiota of grass carp have addressed the microbial response to diet or starvation or the effect of microbes on metabolism; however, the intestinal microbiota of crisp grass carp has yet to be elucidated. Moreover, the specific bacteria that play a role in the crispiness of grass carp fed faba beans have not been elucidated. In the present study, 16S sequencing was carried out to compare the intestinal microbiota in the fore-, mid- and hind-intestine segments of grass carp following feeding with either faba beans or formula feed. Our results showed that (1) the hind-intestine presented significant differences in diversity relative to the fore- or midintestine and (2) faba beans significantly increased the diversity of intestinal microbiota, changed the intestinal microbiota structure (Fusobacteria was reduced from 64.26% to 18.24%, while Proteobacteria was significantly increased from 17.75% to 51.99%), and decreased the metabolism of energy, cofactors and vitamins in grass carp. Furthermore, at the genus and species levels, Acinetobacter accounted for 15.09% of the microbiota, and Acinetobacter johnsonii and Acinetobacter radioresistens constituted 3.41% and 2.99%, respectively, which indicated that Acinetobacter of the family Moraxellaceae contributed to changes in the intestinal microbiota structure and could be used as a potential biomarker. These results may provide clues at the intestinal microbiota level to understanding the mechanism underlying the crispiness of grass carp fed faba beans.

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“…In mammals, the transforming growth factor β1 (TGF-β1)/Smads is an important pathway regulating the expression of type 1 collagen. The changes in upstream protein expression including TGF-β1, Smad2 and Smad4 and post-translational modifications can influence the expression of downstream type 1 collagen-related genes(Loeffler et al, 2017) Yu et al (2019). found that the mRNA and protein expressions of TGF-β1, Smad2 and Smad4 in the flesh of grass carp increased with the increase of type I collagen expression (COL1A1 and CoL1A2).…”

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confidence: 99%

Flavonoid‐enriched diets improved the growth and flesh quality of grass carp ( Ctenopharyngodon idellus ) based on metabolomics

Yang

Poolsawat

et al. 2021

Aquacult Nutr

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Flavonoids are a large group of bioactive compounds from plants with 2-phenylchromone as the skeleton, including berries, apples, onions, cocoa and tea. The representative flavonoids mainly include baicalein (BC), rutin (RT), quercetin (QC), tangerine, glycyrrhizin and catechin (CC), which have biological properties like antioxidant, anti-inflammatory and antibacterial (Manach et al., 2004;Zhang et al., 2011). Due to the antioxidant properties, the chronic consumption of foods rich in flavonoids has been reported to promote the body health in human (Mink et al., 2007, Terao, 2009. In aquatic animals, Londero et al. (2020) reported that dietary RT protected silver catfish (Rhamdia quelen) against oxytetracycline-induced liver oxidative stress and apoptosis. Dietary supplementation of RT also

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Smad4-dependent regulation of type I collagen expression in the muscle of grass carp fed with faba bean

Cited by 48 publications

References 45 publications

Effects of dietary methionine on growth performance, muscle nutritive deposition, muscle fibre growth and type I collagen synthesis of on-growing grass carp (Ctenopharyngodon idella)

Effects of dietary methionine on growth performance, muscle nutritive deposition, muscle fibre growth and type I collagen synthesis of on-growing grass carp (Ctenopharyngodon idella)

Intestinal Microbiota of Grass Carp Fed Faba Beans: A Comparative Study

Flavonoid‐enriched diets improved the growth and flesh quality of grass carp ( Ctenopharyngodon idellus ) based on metabolomics

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