Slug regulates E‐cadherin expression in metastatic adenocarcinoma cells isolated from pleural fluid

Li, Xiangnan; Fang, Chang-Qing; Wang, Ye-Lin; Wang, Xiu-Ru; Wang, Enhua; Li, Jianhua

doi:10.1002/dc.21746

Cited by 6 publications

(2 citation statements)

References 39 publications

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“…EMT is one of the effective pathways of tumor cell migration and the most important step of tumor cell invasion and metastasis [ 17 ]. The most important feature is that the expression of E-cadherin in epithelial type is decreased, while the expression of N-cadherin and vimentin in interstitial type is increased [ 18 ]. This is called the "cadherin transition".…”

Section: Discussionmentioning

confidence: 99%

ZNF692 promotes cell proliferation, invasion and migration of human prostate cancer cells by targeting the EMT signaling pathway

Chen,

Li,

et al. 2024

Eur J Med Res

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Background Prostate cancer poses a considerable threat to human health. At present, the mechanism of tumor progression remains unclear. ZNF692 is overexpressed in many tumors, and the high expression of ZNF692 is correlated with tumor aggressiveness and tumor phenotype of prostate cancer, suggesting that ZNF692 may play an important role in tumor biology of prostate cancer. This paper aims to elucidate the relationship between them. Methods The expression level of ZNF692 was verified in normal prostate cells (RWPE-1) and prostate cancer cells (LNCaP, PC3, DU145). PC3 cells were selected to construct the ZNF692 knockout prostate cancer cell line. The changes of cell proliferation, apoptosis, invasion and metastasis were detected by CCK8, Edu staining, Transwell assay and scratch assay. The expression levels of related proteins were detected by Western blot. Results At the cellular level, ZNF692 was overexpressed to varying degrees in prostate cancer cell lines, with the highest expression in PC3 cell lines. CCK8 and Edu results showed that the proliferation of prostate cancer PC3 cells that knocked down ZNF692 was slowed. Transwell assay and scratch assay showed reduced invasion and migration of prostate cancer PC3 cells that knocked out ZNF692. Flow cytometry showed that the apoptosis rate of prostate cancer PC3 cells after ZNF692 knockout was increased. In addition, after ZNF692 silencing, the expression level of epithelial phenotype E-cadherin increased in PC3 cells, while the expression level of interstitial phenotype N-cadherin, Vimentin, c-Myc, and CyclinA1 decreased. The state of prostate cancer PC3 cells that overexpressed ZNF692 was reversed from the state after ZNF692 was knocked down. Conclusion ZNF692 can be used as a new prognostic marker and a potential biologic therapeutic target for PCa. By inhibiting the expression of c-myc and cyclinA1, the EMT signaling pathway is regulated to provide evidence for its potential molecular mechanism.

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Section: Discussionmentioning

confidence: 99%

ZNF692 promotes cell proliferation, invasion and migration of human prostate cancer cells by targeting the EMT signaling pathway

Chen,

Li,

et al. 2024

Eur J Med Res

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“…13 Numerous studies have shown that E-cadherin may represent a potentially valuable marker of malignant effusion. 14–17 E-Cadherin was found to be highly expressed in pleural effusions from patients with primary lung adenocarcinoma, small cell lung cancer, breast cancer and ovarian cancer. 17–19 However, these findings remain controversial.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy of E-cadherin for malignanteffusions: a systematic review and meta-analysis

Chen

Dai

et al. 2019

Postgraduate Medical Journal

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BackgroundThe differential diagnosis of malignant effusion remains a clinical challenge. We aim to summarise all relevant literature studies in order to determine the overall clinical value of E-cadherin in the diagnosis of malignant effusion by meta-analysis.MethodsPubMed, the Cochrane Library Database, Medline (Ovid), Web of Science, CNKI, WANFANG and WEIPU databases are thoroughly searched up to 15 March2018. The calculated pooled sensitivity, specificity, likelihood ratio (LR), diagnostic OR(DOR) and the summary receiver operating characteristic (SROC) curvewere plotted.ResultsA total of 15 studies were included in the analysis. The sensitivity and specificity of E-cadherin in the diagnosis of malignant effusion were determined to be high, with a sensitivity of 0.83(95%CI0.79 to 0.87) and a specificity of 0.96(95%CI0.90 to 0.98). The positive LR was determined to be 21.10(95%CI 8.54 to 52.11), the negative LR was determined to be 0.17(95% CI 0.14 to 0.22) and the DOR was determined to be 121.34(95%CI 49.11 to 299.80). The SROC curve exhibited a high overall diagnostic, with the area under the curve measured to be 0.91(95% CI 0.89 to 0.93). Subgroup analysis showed the method (cell blocks or smears), sample size (≥100 or<100), geographical location (Asia, Europe or USA) and impact factor of each article (≥3 or<3) were not the sources of overall heterogeneity.ConclusionE-cadherin exhibits very good diagnostic accuracy for the diagnosis for malignant effusion; thus, it can be helpful in the process of clinical decisions.

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Genitourinary System Tumors

Iczkowski

Harding-Jackson

2015

Molecular Oncology Testing for Solid Tumors

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Slug regulates E‐cadherin expression in metastatic adenocarcinoma cells isolated from pleural fluid

Cited by 6 publications

References 39 publications

ZNF692 promotes cell proliferation, invasion and migration of human prostate cancer cells by targeting the EMT signaling pathway

ZNF692 promotes cell proliferation, invasion and migration of human prostate cancer cells by targeting the EMT signaling pathway

Diagnostic accuracy of E-cadherin for malignanteffusions: a systematic review and meta-analysis

Genitourinary System Tumors

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