SlSNAT Interacts with HSP40, a Molecular Chaperone, to Regulate Melatonin Biosynthesis and Promote Thermotolerance in Tomato

Wang, Xiaoyun; Zhang, Haijun; Xie, Qian; Liu, Ying; Lv, Hongmei; Bai, Ru-Yue; Ma, Rui; Li, Xiangdong; Zhang, Xichun; Guo, Yang‐Dong; Zhang, Na

doi:10.1093/pcp/pcaa018

Cited by 63 publications

(46 citation statements)

References 52 publications

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“…Suppression of SNAT1 led to a decrease in melatonin, followed by enhanced pathogen susceptibility [6], salinity hypersensitivity [8], and susceptibility to high-light stress [7]. SNAT1 OE increased melatonin levels, resulting in cadmium tolerance [51], delayed senescence [14], lateral root promotion [52], salt tolerance [53], and thermotolerance [16]. In addition to the genetic evidence, exogenous melatonin treatment conferred stress tolerance to a wide range of biotic and abiotic stressors, including viral attack [8,54,55].…”

Section: Melatonin and Photosynthesismentioning

confidence: 98%

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Melatonin Regulates Chloroplast Protein Quality Control via a Mitogen-Activated Protein Kinase Signaling Pathway

Lee

Back

2021

Antioxidants

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Serotonin N-acetyltransferase 1 (SNAT1), the penultimate enzyme for melatonin biosynthesis has shown N-acetyltransferase activity toward multiple substrates, including histones, serotonin, and plastid proteins. Under two different light conditions such as 50 or 100 μmol m−2 s−1, a SNAT1-knockout (snat1) mutant of Arabidopsis thaliana ecotype Columbia (Col-0) exhibited small size phenotypes relative over wild-type (WT) Arabidopsis Col-0. Of note, the small phenotype is stronger when growing at the 50 μmol m−2 s−1, exhibiting a dwarfism phenotype and delayed flowering. The snat1 Arabidopsis Col-0 accumulated less starch than the WT Col-0. Moreover, snat1 exhibited lower Lhcb1, Lhcb4, and RBCL protein levels, compared with the WT Col-0, but no changes in the corresponding transcripts, suggesting the involvement of melatonin in chloroplast protein quality control (CPQC). Accordingly, caseinolytic protease (Clp) and chloroplast heat shock proteins (CpHSPs), two key proteins involved in CPQC, as well as ROS defense were suppressed in snat1. In contrast, exogenous melatonin treatment induced expression of Clp, CpHSP, APX1, and GST, but not other growth-related genes such as DWF4, KS, and IAA1. Finally, the induction of ClpR1, APX1, and GST1 in response to melatonin was inhibited in the mitogen-activated protein kinase (MAPK) knockdown Arabidopsis (mpk3/6), suggesting that melatonin-mediated CPQC was mediated, in part, by the MAPK signaling cascade. These results suggest that melatonin is involved in CPQC, which plays a pivotal role in starch synthesis in plants.

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Section: Melatonin and Photosynthesismentioning

confidence: 98%

“…Overall, SNAT1 plays a pivotal role in melatonin biosynthesis in many plant species, including rice. Its overexpression (OE) leads to increased melatonin production, whereas its suppression results in decreased melatonin production and the corresponding physiological and biochemical phenotypes described above [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Melatonin Regulates Chloroplast Protein Quality Control via a Mitogen-Activated Protein Kinase Signaling Pathway

Lee

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2021

Antioxidants

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“…Histochemical staining by diaminobenzidine (DAB) and 2 0 ,7 0dichlorodihydrofluorescein diacetate (H 2 DCFDA) were used to analyse the accumulation of H 2 O 2 (X. Wang et al, 2020). For DAB staining, 15 fresh leaves from three plants were sampled and immersed in 1 mg/ml DAB solution (Sigma-Aldrich, D5905), after 4 hr of incubation, leaves were bleached in a bleaching solution.…”

Section: Analysis Of Electrolyte Leakage Malondialdehyde and Ros Amentioning

confidence: 99%

SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication

Zhang

Liu

et al. 2020

Plant Cell & Environment

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Improving plant water-use efficiency (WUE) is important to plant survival and crop yield in the context of water limitation. In this study, SlTLFP8 (Tubby-like F-box protein 8) was identified as an osmotic-induced gene in tomato. Transgenic tomato with up-regulated expression of SlTLFP8 showed enhanced water-deficient resistance, whereas knockout mutants generated by CRISPR/Cas9 were more sensitive to water deficit. SlTLFP8 overexpression significantly enhanced WUE by suppressing transpiration under both water-sufficient and water-deficient conditions. Further study showed that overexpressing SlTLFP8 significantly increased leaf epidermal cell size and thereby decreased stomatal density 10-20%, conversely SlTLFP8 knockout resulted in decreased cell size and thereby increased stomatal density 20-50%. SlTLFP8 overexpression and knockout modulated ploidy levels in leaf cells. Changes in expression of cell cycle related genes also indicated that SlTLFP8 affected cell size and stomatal density through endocycle transition. Despite changes in stomata density and transpiration, altering the expression of SlTLFP8 did not change photosynthesis. Additionally, biomass was not altered and there was little difference in fruit yield for transgenic and wild type lines under water-sufficient and water-deficient conditions. Our results demonstrate the effect of SlTLFP8 on endoreduplication and the potential of SlTLFP8 for improvement of WUE. Brief Summery This work found a new mechanism of TLP (Tubby like protein) response to waterdeficient stress. SlTLFP8, a member of TLP family, regulates water-deficient resistance by modulating water loss via affecting stomatal density. Expression of SlTLFP8 was induced by osmotic stress. Transgenic tomato lines with SlTLFP8 overexpression or SlTLFP8 knockout showed significantly differences in water-use efficiency (WUE) and water-deficient resistance. The difference of leaf water loss caused by transpiration is the main explanation of the difference in WUE and water-deficient resistance. Additionally, overexpressing SlTLFP8 significantly decreased stomatal density, while SlTLFP8 knockout resulted in increased stomatal density, and SlTLFP8 affected stomatal density through endoreduplication and altered epidermal cell size. Despite

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“…Chaperone proteins are a class of proteins related to protein degradation and folding that act as molecular chaperones to improve resistance against environmental stress (Wang et al., 2020; Wei, Liu, Hu, Yan, & Shi, 2020). Our results showed that the expression of chaperone DnaJ 16 significantly increased in response to high temperature stress.…”

Section: Discussionmentioning

confidence: 99%

iTRAQ‐based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress

Liu

Lin

et al. 2020

Aquac. Res.

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Global warming increases seawater temperature, causing high temperature stress to marine organisms, including algae. This study aimed to explore the global proteomic response of Sargassum fusiforme under high temperature stress. Sargassum fusiforme seedlings were cultured in natural seawater for 24 hr and subjected to different temperatures (22°C, control group; 27°C and 32°C, high temperature stress group) for 1, 3, 5 and 7 days. Changes in their membrane lipid peroxidation after high temperature stress were investigated. Proteomic changes in the air bladders of S. fusiforme were analysed using isobaric tags for relative and absolute quantification, along with liquid chromatography-tandem mass spectrometry. Data were analysed using bioinformatics methods. Results showed that high temperature stress destroyed the cell membrane of the air bladders. Further, 28 and 53 differentially expressed proteins (DEPs) were found in the 27°C and 32°C treatment groups respectively. These DEPs were mainly involved in glycolysis, single-organism catabolism, purine nucleoside diphosphate metabolism and carbohydrate catabolism. In addition, DEPs were significantly enriched in 10 pathways, such as glycolytic process, biosynthesis of antibiotics, ribosome, biosynthesis of secondary metabolites and biosynthesis of amino acids. Proteomics analyses indicated that proteins associated with synthesis, folding, degradation, photosynthesis and energy and carbohydrate metabolism are differentially expressed under high temperature stress and normal conditions.

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SlSNAT Interacts with HSP40, a Molecular Chaperone, to Regulate Melatonin Biosynthesis and Promote Thermotolerance in Tomato

Cited by 63 publications

References 52 publications

Melatonin Regulates Chloroplast Protein Quality Control via a Mitogen-Activated Protein Kinase Signaling Pathway

Melatonin Regulates Chloroplast Protein Quality Control via a Mitogen-Activated Protein Kinase Signaling Pathway

SlTLFP8 reduces water loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication

iTRAQ‐based quantitative proteomic analysis ofSargassum fusiformein response to high temperature stress

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