SLS Crystallization Platform at Beamline X06DA—A Fully Automated Pipeline Enabling <i>in Situ</i> X-ray Diffraction Screening

Bingel-Erlenmeyer, R.; Oliéric, V.; Grimshaw, James; Gabadinho, José; Wang, X.; Ebner, Simon Gregor; Isenegger, A.; Schneider, Roman; Schneider, J.R.; Glettig, Wayne; Pradervand, Claude; Panepucci, Ezequiel; Tanaka, Takashi; Wang, M.; Schulze-Briese, Clemens

doi:10.1021/cg101375j

Cited by 92 publications

(76 citation statements)

References 27 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, COC has seen widespread adoption as the polymer film of choice for X-ray compatible devices, 46 including simple channel structures for counterdiffusion, 56,58,60,61,126 droplet-based devices, 108,109,118 and larger-scale X-ray compatible wellplates. [127][128][129][130][131][132][133][134][135][136][137][138][139][140] However, further decreasing the device thickness to achieve the signal-to-noise levels required for microcrystallography is a significant materials' challenge. Typical reports of X-ray compatible microfluidics describe results where the path length of the device materials is nearly twice that of the crystal of interest.…”

Section: B Device Materials For Microcrystallographymentioning

confidence: 99%

Microfluidics: From crystallization to serial time-resolved crystallography

Sui

Perry

2017

Structural Dynamics

View full text Add to dashboard Cite

Capturing protein structural dynamics in real-time has tremendous potential in elucidating biological functions and providing information for structure-based drug design. While time-resolved structure determination has long been considered inaccessible for a vast majority of protein targets, serial methods for crystallography have remarkable potential in facilitating such analyses. Here, we review the impact of microfluidic technologies on protein crystal growth and X-ray diffraction analysis. In particular, we focus on applications of microfluidics for use in serial crystallography experiments for the time-resolved determination of protein structural dynamics.

show abstract

Section: B Device Materials For Microcrystallographymentioning

confidence: 99%

Microfluidics: From crystallization to serial time-resolved crystallography

Sui

Perry

2017

Structural Dynamics

View full text Add to dashboard Cite

show abstract

“…As the data collected at room temperature is free of artefacts from handling, cryo-protectants and from the cooling process itself, in situ data collection can provide a rapid means of optimising crystallisation conditions. The first implementations used a six-axis robotic arm with a gripper for SBS format crystallization plates to position and rotate the plates in the X-ray beam (Jacquamet, Ohana, Joly, Borel , et al , 2004), while more recent implementations have incorporated dedicated goniometry into the endstation (Bingel-Erlenmeyer et al , 2011, le Maire et al , 2011, Axford et al , 2012). Crystallisation plates have also evolved to facilitate X-ray data collection, with plates designed to minimise background scatter and maximise the accessible rotation range becoming commercially available.…”

Section: Endstation and Experimentsmentioning

confidence: 99%

“…In recent years there has been a resurgence in room temperature (RT) crystallography at synchrotron sources with facilities for in situ data collection developed at a number of sources (Jacquamet, Ohana, Joly, Legrand , et al , 2004, Bingel-Erlenmeyer et al , 2011, Axford et al , 2012). Data collection from crystals held in a crystallisation tray means the potentially damaging steps of crystal harvesting and the cryo-cooling process can be sidestepped.…”

Section: Endstation and Experimentsmentioning

confidence: 99%

Current advances in synchrotron radiation instrumentation for MX experiments

Owen

Juanhuix

Fuchs

2016

Archives of Biochemistry and Biophysics

View full text Add to dashboard Cite

Following pioneering work 40 years ago, synchrotron beamlines dedicated to macromolecular crystallography (MX) have improved in almost every aspect as instrumentation has evolved. Beam sizes and crystal dimensions are now on the single micron scale while data can be collected from proteins with molecular weights over 10 MDa and from crystals with unit cell dimensions over 1000 Å. Furthermore it is possible to collect a complete data set in seconds, and obtain the resulting structure in minutes. The impact of MX synchrotron beamlines and their evolution is reflected in their scientific output, and MX is now the method of choice for a variety of aims from ligand binding to structure determination of membrane proteins, viruses and ribosomes, resulting in a much deeper understanding of the machinery of life. A main driving force of beamline evolution have been advances in almost every aspect of the instrumentation comprising a synchrotron beamline. In this review we aim to provide an overview of the current status of instrumentation at modern MX experiments. The most critical optical components are discussed, as are aspects of endstation design, sample delivery, visualization and positioning, the sample environment, beam shaping, detectors and data acquisition and processing.

show abstract

“…The FIP beamline at the ESRF was the first to offer access to automated in situ modes and to integrate 6-axis robotic arms that are now widely used for plate-screening at many synchrotrons (Jacquamet et al, 2004). Later, X06DA at the Swiss Light Source was expressly conceived as an automated in situ screening facility with an integrated crystallisation platform (Bingel-Erlenmeyer et al, 2011). I24 at Diamond has since laid much of the ground-work for in situ diffraction using micro-beams and has firmly established this technique for membrane protein crystallography (Axford et al, 2012).…”

Section: In Situ Diffraction and Microfluidicsmentioning

confidence: 99%

Generation X-ray – A coming of age

Fox¹

2015

Arbor

View full text Add to dashboard Cite

ABSTRACT:In 2014, the scientific community celebrated the impact crystallography has had on fundamental and applied science, and the ground-breaking discoveries that have gifted us with vision into the molecular and nano-worlds. UNESCO has declared 2015 as the International Year of Light, so now is perhaps a well timed moment to reflect on the role synchrotrons play in the field. Access to synchrotron radiation has revolutionized our ability to explore the properties, interactions and structure of materials, and broadened our understanding in diverse scientific arenas ranging from our cultural heritage, to nano-technology and new materials, to cellular systems and drug discovery. The range of potential synchrotron applications and fields of interest is enormous and beyond the scope of any single article, so my aim here, is simply to provide a snapshot filtered through the lens of biological crystallography. We will look back at how synchrotrons developed into the state-ofthe-art facilities we see today, how beamlines adapted to the quickening brought on by the introduction of high throughput techniques at the turn of the millennium, and forward to some of the new directions and technologies that are transforming modern light sources and crystallography.KEYWORDS: beamline; light sources; membrane protein; microfluidics; protein crystallography; serial crystallography; structural biology; synchrotron; XFEL. RESUMEN:La comunidad científica celebró en 2014 el impacto que la cristalografía ha tenido sobre la ciencia fundamental y aplicada, así como los de descubrimientos relevantes que nos han proporcionado una visión del mundo a escala molecular y nanométri-ca. La UNESCO ha declarado 2015 como el Año Internacional de la Luz por lo que quizás es un buen momento para reflexionar sobre el papel que han jugado y juegan los sincrotrones en esta ciencia. El acceso a la radiación sincrotrón ha revolucionado nuestra capacidad para explorar las propiedades, interacciones y estructura de materiales, así como ampliado nuestro conocimiento en distintos contextos científicos, desde el patrimonio cultural a la nanotecnología y nuevos materiales, hasta la exploración de sistemas celulares y búsqueda de fármacos. El rango de potenciales aplicaciones de los sincrotrones y sus campos de interés es vasto y queda fuera del alcance de un único artículo por lo que la intención del autor es la de proporcionar una imagen de los sincrotrones obtenida a través del filtro de la cristalografía macromolecular. Echaremos la vista atrás para ver cómo los sincrotrones han ido evolucionado hasta llegar a las actuales instalaciones en donde las líneas de trabajo asociadas a los mismos se están adaptado rápidamente a la introducción de las técnicas de alto rendimiento en el cambio del milenio; pero también miraremos hacia el futuro, hacia las nuevas tendencias y tecnologías que ya están transformando las actuales instalaciones y la misma cristalografía.PALABRAS CLAVE: biología estructural, cristalografía de proteínas, cristalografía en serie, línea, ...

show abstract

SLS Crystallization Platform at Beamline X06DA—A Fully Automated Pipeline Enabling in Situ X-ray Diffraction Screening

Cited by 92 publications

References 27 publications

Microfluidics: From crystallization to serial time-resolved crystallography

Microfluidics: From crystallization to serial time-resolved crystallography

Current advances in synchrotron radiation instrumentation for MX experiments

Generation X-ray – A coming of age

Contact Info

Product

Resources

About