“…Also, peaks 6 and 17 showed height but less than peak 18 with peak areas of 10.447 and 10.019% at retention times of 10.514 and 45.532min respectively in addition to several minor peaks representing other compounds in little amounts. These peaks (which consisted of compounds like 1,2,3,4,6-Pentatrimethylsilyl Glucopyranose, Trimethylsilyl ether-glucitol and Octadecanamide, N-(2-methyl propyl)-N-nitroso) could be attributable to amino acid residues in protein molecules, since these compounds are linear nitrogenous compounds have long carbon chains (C16-C18) which can contribute with better interaction and can be protonated in the presence of other compounds (Rocha et al, 2020), indicating the production of certain proteinic substances into cell-free filtrate of Aspergillus niger DS22 (ON076463.1). On the other side, as a result of its anti-angiogenesis implications and controlling functions of signal pathways such as autophagy, apoptosis, and the arrest of the cell cycle of cancer cells, oridonin is frequently utilized for relieving pain and inflammation associated with cancer such as carcinoma of the liver, cancer of the breast, carcinoma of the gall bladder, cervical carcinoma, Several bioactive Compared with other fungi, Aspergillus species is one of the most prevalent Antiangiogenic, antioxidant, and antitumorigenic effectiveness (Niero et al, 2013;Mashraqi et al, 2023).…”