Slow growth rate of mycobacteria

Lewin, Astrid; Sharbati, Soroush

doi:10.1007/s00103-005-1171-x

Cited by 9 publications

(6 citation statements)

References 55 publications

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“…However, those assays are heavily dependent on the cultivation of the microbes prior to analysis. This is unfavourable in the case of highly pathogenic mycobacteria species, which are all notorious for being slow‐growing bacteria with generation times of up to 30 days .…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopic identification of Mycobacterium tuberculosis

Stöckel

Meisel

Lorenz

et al. 2016

Journal of Biophotonics

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In this study, Raman microspectroscopy has been utilized to identify mycobacteria to the species level. Because of the slow growth of mycobacteria, the per se cultivation-independent Raman microspectroscopy emerges as a perfect tool for a rapid on-the-spot mycobacterial diagnostic test. Special focus was laid upon the identification of Mycobacterium tuberculosis complex (MTC) strains, as the main causative agent of pulmonary tuberculosis worldwide, and the differentiation between pathogenic and commensal nontuberculous mycobacteria (NTM). Overall the proposed model considers 26 different mycobacteria species as well as antibiotic susceptible and resistant strains. More than 8800 Raman spectra of single bacterial cells constituted a spectral library, which was the foundation for a two-level classification system including three support vector machines. Our model allowed the discrimination of MTC samples in an independent validation dataset with an accuracy of 94% and could serve as a basis to further improve Raman microscopy as a first-line diagnostic point-of-care tool for the confirmation of tuberculosis disease.

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Section: Introductionmentioning

confidence: 99%

Raman spectroscopic identification of Mycobacterium tuberculosis

Stöckel

Meisel

Lorenz

et al. 2016

Journal of Biophotonics

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“…As we discussed earlier, the growth rate of mycobacteria may contribute to their pathogenicity [14]. Hence, it can be suggested that the low porin expression in M. fortuitum strains isolated from human patients compared to saprophytic species of RGM like M. smegmatis contributes to higher pathogenicity caused by an enhanced ability to multiply intracellularly.…”

Section: Discussionmentioning

confidence: 99%

“…We were able to show that the porin pathway affects the intracellular persistence of different species in different ways. The findings suggest that intracellular persistence of mycobacteria depends, inter alia, on the balance between "walling-off" towards the hostile environment and the uptake of required compounds in the nutrient-depleted phagosomal environment [5,13,14]. To further examine this hypothesis, we are searching for more appropriate models.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of porin genes from Mycobacterium fortuitumand their impact on growth

et al. 2009

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BackgroundHighly pathogenic mycobacteria like Mycobacterium tuberculosis are characterised by their slow growth and their ability to reside and multiply in the very hostile phagosomal environment and a correlation between the growth rate of mycobacteria and their pathogenicity has been hypothesised. Here, porin genes from M. fortuitum were cloned and characterised to address their impact on the growth rate of fast-growing and pathogenic mycobacteria.ResultsTwo genes encoding porins orthologous to MspA from M. smegmatis, porM1 and porM2, were cloned from M. fortuitum strains, which were originally isolated from human patients. Both porin genes were at least partially able to complement the mutations of a M. smegmatis mutant strain lacking the genes mspA and mspC with respect to the growth rate. PorM1 and porM2 were present in different strains of M. fortuitum including the type strain. Comparative expression analysis of porM genes revealed divergent porin expression among analysed M. fortuitum strains. Repression of the expression of porins by antisense technique decreased the growth rates of different M. fortuitum. The effects of over-expression of porM1 as well as porM2 varied depending on the strain and the concentration of antibiotic added to the medium and indicated that PorM1 and PorM2 enhance the growth of M. fortuitum strains, but also the diffusion of the antibiotic kanamycin into the cells.ConclusionThis study demonstrates the important role of porin expression in growth as well as antibiotic susceptibility of the opportunistic bacterium M. fortuitum.

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“…Several genes like mce1 (mycobacterial cell entry protein) and phthiocerol synthesis polyketide synthase type I (ppsA and ppsB), which are associated with the virulence property of the mycobacteria, are also involved in their slow growth (Beste et al 2009). Lewin et al 2005 demonstrated all the highly pathogenic Mycobacterium species belong to the risk group 3 exhibit slow-growing property (Lewin and Sharbati-Tehrani 2005). It indicates a strong connection between the pathogenicity and the slow growth of the bacteria.…”

Section: Genome Sequence Alignment Comparison Of Orthologous Gene CLmentioning

confidence: 99%

The draft genome of a new Verminephrobacter eiseniae strain: a nephridial symbiont of earthworms

et al. 2020

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Purpose: Verminephrobacter is a genus of symbiotic bacteria that live in the nephridia of earthworms. The bacteria are recruited during the embryonic stage of the worm and transferred from generation to generation in the same manner. The worm provides shelter and food for the bacteria. The bacteria deliver micronutrients to the worm. The present study reports the genome sequence assembly and annotation of a new strain of Verminephrobacter called Verminephrobacter eiseniae msu. Methods: We separated the sequences of a new Verminephrobacter strain from the whole genome of Eisenia fetida using the sequence of V. eiseniae EF01-2, and the bacterial genome was assembled using the CLC Workbench. The de novo-assembled genome was annotated and analyzed for the protein domains, functions, and metabolic pathways. Besides, the multigenome comparison was performed to interpret the phylogenomic relationship of the strain with other proteobacteria. Result: The FastqSifter sifted a total of 593,130 Verminephrobacter genomic reads. The de novo assembly of the reads generated 1832 contigs with a total genome size of 4.4 Mb. The Average Nucleotide Identity denoted the bacterium belongs to the species V. eiseniae, and the 16S rRNA analysis confirmed it as a new strain of V. eiseniae. The AUGUSTUS genome annotation predicted a total of 3809 protein-coding genes; of them, 3805 genes were identified from the homology search. Conclusion: The bioinformatics analysis confirmed the bacterium is an isolate of V. eiseniae, and it was named Verminephrobacter eiseniae msu. The whole genome of the bacteria can be utilized as a useful resource to explore the area of symbiosis further.

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Slow growth rate of mycobacteria

Cited by 9 publications

References 55 publications

Raman spectroscopic identification of Mycobacterium tuberculosis

Raman spectroscopic identification of Mycobacterium tuberculosis

Characterisation of porin genes from Mycobacterium fortuitumand their impact on growth

The draft genome of a new Verminephrobacter eiseniae strain: a nephridial symbiont of earthworms

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