Slope-induced factors shape bacterial communities in surface soils in a forested headwater catchment

Diversity profiling of soil bacterial and fungal communities in the Ogasawara (Bonin) Islands, Japan

“…Each sample was placed in a sterile sampling bag, and then kept in a cooler with ice until transported to the laboratory, where it was stored at −80°C until downstream analysis. Soil DNA was extracted using the NucleoSpin Soil DNA extraction kit (Macherey-Nagel, Düren, Germany), after which amplicon libraries were prepared by a two-step tailed PCR procedure as described in our previous studies ( 4 , 5 ). The 515F/806R primer pair [5′-GTGCCAGCMGCCGCGGTAA-3′/5′-GGACTACHVGGGTWTCTAAT-3′; ( 6 )] was chosen to target the V4 hypervariable region of the prokaryotic 16S rRNA gene.…”