SlMYB72 Regulates the Metabolism of Chlorophylls, Carotenoids, and Flavonoids in Tomato Fruit

Wu, Mengbo; Xu, Xin; Hu, Xiaowei; Liu, Yudong; Cao, Haohao; Chan, Helen M.; Gong, Zehao; Yuan, Yujin; Luo, Yingqing; Feng, Bihong; Li, Zhengguo; Deng, Wei

doi:10.1104/pp.20.00156

Cited by 130 publications

(97 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to obtain the accurate function of the hub MYBs in a coexpression network, we used these MYBs as cue blast against TAIR database, and many previous published works that claimed MYBs participated in flavonoids, anthocyanins, or carotenoids biosynthesis (Mehrtens et al, 2005;Stracke et al, 2010;Ampomah-Dwamena et al, 2019;Meng et al, 2019;Wu et al, 2020), and three MYBs including c44135 (MYB-1), c40864 (MYB-2), and c39130 (MYB-3), which showed the highest expression level in BO, HL, and WL were closest match with these well-documented MYBs. Phylogenetic analysis indicated that MYB-1 belongs to the clade S7 including AtMYB1, AtMYB11, AtMYB12, and AtMYB111, which regulate the production of phenylpropanoids and flavonol (Mehrtens et al, 2005;Stracke et al, 2010;Peng et al, 2016).…”

Section: Coexpression Network Analysismentioning

confidence: 99%

“…Phylogenetic analysis indicated that MYB-1 belongs to the clade S7 including AtMYB1, AtMYB11, AtMYB12, and AtMYB111, which regulate the production of phenylpropanoids and flavonol (Mehrtens et al, 2005;Stracke et al, 2010;Peng et al, 2016). MYB-2 was clustered with AtMYB123 (S7 clade) that acts as a key determinant in the proanthocyanidin accumulation of developing seed (Ampomah-Dwamena et al, 2019;Meng et al, 2019;Wu et al, 2020). MYB3 was clustered with AtMYB108, AtMYB78 in clade S20, which participated in stress treatment and anther development (Christian et al, 2010;Peng et al, 2016).…”

Section: Coexpression Network Analysismentioning

confidence: 99%

“…Recently, several transcription factors families have been demonstrated to directly regulate the carotenogenic genes and control carotenoid biosynthesis in several model plants. In the tomato fruit, downregulation of SlMYB72 altered the expression levels of genes involved in the biosynthesis of chlorophylls, carotenoids, and flavonoids (Wu et al, 2020). In Arabidopsis, the bHLH transcription factor PHYTOCHROME INTERACTING FACTOR1 (PIF1) and the bZIP TF LONG HYPOCOTYL5 (HY5) antagonistically regulate carotenoid accumulation by directly binding to the promoter of PSY in response to light stimulation (Toledo-Ortiz et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Metabolites and Transcripts Involved in Flower Pigmentation in Primula vulgaris

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

Primula vulgaris exhibits a wide range of flower colors and is a valuable ornamental plant. The combination of flavonols/anthocyanins and carotenoids provides various colorations ranging from yellow to violet-blue. However, the complex metabolic networks and molecular mechanisms underlying the different flower colors of P. vulgaris remain unclear. Based on comprehensive analysis of morphological anatomy, metabolites, and gene expression in different-colored flowers of P. vulgaris, the mechanisms relating color-determining compounds to gene expression profiles were revealed. In the case of P. vulgaris flower color, hirsutin, rosinin, petunidin-, and cyanidin-type anthocyanins and the copigment herbacetin contributed to the blue coloration, whereas peonidin-, cyandin-, and delphinidin-type anthocyanins showed high accumulation levels in pink flowers. The color formation of blue and pink were mainly via the regulation of F3′5′H (c53168), AOMT (c47583, c44905), and 3GT (c50034). Yellow coloration was mainly due to gossypetin and carotenoid, which were regulated by F3H (c43100), F3 1 (c53714), 3GT (c53907) as well as many carotenoid biosynthetic pathway-related genes. Co-expression network and transient expression analysis suggested a potential direct link between flavonoid and carotenoid biosynthetic pathways through MYB transcription factor regulation. This work reveals that transcription changes influence physiological characteristics, and biochemistry characteristics, and subsequently results in flower coloration in P. vulgaris.

show abstract

Section: Coexpression Network Analysismentioning

confidence: 99%

Section: Coexpression Network Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Metabolites and Transcripts Involved in Flower Pigmentation in Primula vulgaris

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Most of the transcript factors play important roles in developmental processes in plants [ 23 ]. In tomato fruit, SlMYB72 directly targets protochlorophyllide reductase, Mg-chelatase H subunit, and knotted1-like homeobox2 genes and regulates chlorophyll biosynthesis and chloroplast development [ 24 ]. Kiwifruit MYB7 plays a role in modulating carotenoid and chlorophyll pigment accumulation in tissues through transcriptional activation of metabolic pathway genes [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Photosystem Disorder Could be the Key Cause for the Formation of Albino Leaf Phenotype in Pecan

Zhang

Wang

Jia

et al. 2020

IJMS

View full text Add to dashboard Cite

Pecan is one of the most famous nut species in the world. The phenotype of mutants with albino leaves was found in the process of seeding pecan, providing ideal material for the study of the molecular mechanisms leading to the chlorina phenotype in plants. Both chlorophyll a and chlorophyll b contents in albino leaves (ALs) were significantly lower than those in green leaves (GLs). A total of 5171 differentially expression genes (DEGs) were identified in the comparison of ALs vs. GLs using high-throughput transcriptome sequencing; 2216 DEGs (42.85%) were upregulated and 2955 DEGs (57.15%) were downregulated. The expressions of genes related to chlorophyll biosynthesis (HEMA1, encoding glutamyl-tRNA reductase; ChlH, encoding Mg-protoporphyrin IX chelatase (Mg-chelatase) H subunit; CRD, encoding Mg-protoporphyrin IX monomethylester cyclase; POR, encoding protochlorophyllide reductase) in ALs were significantly lower than those in GLs. However, the expressions of genes related to chlorophyll degradation (PAO, encoding pheophorbide a oxygenase) in ALs were significantly higher than those in GLs, indicating that disturbance of chlorophyll a biosynthesis and intensification of chlorophyll degradation lead to the absence of chlorophyll in ALs of pecan. A total of 72 DEGs associated with photosynthesis pathway were identified in ALs compared to GLs, including photosystem I (15), photosystem II (19), cytochrome b6-f complex (3), photosynthetic electron transport (6), F-type ATPase (7), and photosynthesis-antenna proteins (22). Moreover, almost all the genes (68) mapped in the photosynthesis pathway showed decreased expression in ALs compared to GLs, declaring that the photosynthetic system embedded within the thylakoid membrane of chloroplast was disturbed in ALs of pecan. This study provides a theoretical basis for elucidating the molecular mechanism underlying the phenotype of chlorina seedlings of pecan.

show abstract

“…Emerging evidences indicated that R2R3-MYB TFs also participate in tomato ripening. The overexpression of SlMYB72 in tomato while did not affect carotenoid accumulation, its suppression leads to a reduction in lycopene and β-carotene level (Wu et al, 2020). Likewise, repression of SlMYB70 accelerated fruit ripening, while overexpression delayed fruit ripening (Cao et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Seeing the unseen: A trifoliate (MYB117) mutant allele fortifies folate and carotenoids in tomato fruits

Tyagi

Sunkum

Rai

et al. 2021

Preprint

View full text Add to dashboard Cite

Micronutrient deficiency also termed hidden hunger affects a large segment of the human population, particularly in developing and underdeveloped nations. Tomato the second most consumed vegetable crop in the world after potato can serve as a sustainable source to alleviate micronutrient deficiency. In tomato, the mutations in the R2R3-MYB117 transcription factor elicit trifoliate leaves and initiate axillary meristems; however, its effect on fruit metabolome remains unexplored. The fruits of a new trifoliate (tf) allele (tf-5) were firmer, had higher Brix, folate, and carotenoids. The transcriptome, proteome, and metabolome profiling of tf-5 reflected a broad-spectrum change in homeostasis. The tf-5 allele enhanced the fruit firmness by suppressing cell wall softening-related proteins. The tf-5 fruit displayed a substantial increase in aminome, particularly γ-aminobutyric acid, with a parallel reduction in aminoacyl t-RNA synthases. The increased lipoxygenases proteins and transcripts seemingly elevated jasmonic acid. In addition, increased abscisic acid hydrolases transcripts coupled with reduced precursor supply lowered abscisic acid. The upregulation of carotenoids was mediated by modulation of methylerythreitol and plastoquinone pathways along with an increase in carotenoids isomerization proteins. The upregulation of folate in tf-5 was connoted by the increase in precursor p-aminobenzoic acid and transcripts of several folate biosynthesis pathway genes. The reduction in pterin-6-carboxylate and γ-glutamyl hydrolase activity indicated that the diminished folate degradation also enriched folate levels. Our study delineates that introgression of the tf-5 can be used for the γ-aminobutyric acid, carotenoids, and folate fortification of tomato.

show abstract

SlMYB72 Regulates the Metabolism of Chlorophylls, Carotenoids, and Flavonoids in Tomato Fruit

Cited by 130 publications

References 62 publications

Characterization of Metabolites and Transcripts Involved in Flower Pigmentation in Primula vulgaris

Characterization of Metabolites and Transcripts Involved in Flower Pigmentation in Primula vulgaris

Photosystem Disorder Could be the Key Cause for the Formation of Albino Leaf Phenotype in Pecan

Seeing the unseen: A trifoliate (MYB117) mutant allele fortifies folate and carotenoids in tomato fruits

Contact Info

Product

Resources

About