SlMYB7, an AtMYB4-Like R2R3-MYB Transcription Factor, Inhibits Anthocyanin Accumulation in <i>Solanum lycopersicum</i> Fruits

Zhang, Li; Duan, Zedi; Ma, Shuang; Sun, Shaokun; Sun, Minghui; Xiao, Yunhong; Ni, Na; Irfan, Muhammad; Chen, Lijing; Sun, Yibo

doi:10.1021/acs.jafc.3c05185

Cited by 8 publications

(4 citation statements)

References 49 publications

(102 reference statements)

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“…In A. thaliana, AtMYB4 in the S4 subgroup and its homologs AtMYB7 and AtMYB32 can also regulate the flavonoid biosynthesis pathway [34,35]. In tomato, SiMYB7, an analog of AtMYB4, was proven to inhibit the accumulation of anthocyanins in fruit [36]. NtMYB9 was found in Narcissus tazetta, which belongs to the branch of AtMYB4, and participated in the positive regulation of flavonoid biosynthesis; it may also promote flavanol biosynthesis [37].…”

Section: Discussionmentioning

confidence: 99%

Identification and Expression Analysis of R2R3-MYB Transcription Factors Associated with Flavonoid Biosynthesis in Panax quinquefolius

Song,

Yan,

Guo

et al. 2024

IJMS

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Panax quinquefolius L. is an important medicinal plant, and flavonoids are among its main secondary metabolites. The R2R3-MYB transcription factor plays an irreplaceable role in plant growth, development, and secondary metabolism. In our study, we identified 159 R2R3-MYBs and analyzed their physical and chemical properties in P. quinquefolius. The protein length of 159 PqMYBs varied from 107 to 1050 amino acids. The molecular weight ranged from 12.21 to 116.44 kDa. The isoelectric point was between 4.57 and 10.34. We constructed a phylogenetic tree of P. quinquefolius and Arabidopsis thaliana R2R3-MYB family members, and PqMYB members were divided into 33 subgroups. Transcriptome data analysis showed that the expression patterns of PqMYBs in root, leaf, and flower were significantly different. Following the MeJA treatment of seedlings, five candidate PqMYB genes demonstrated a response. A correlation analysis of PqMYBs and candidate flavonoid pathway genes showed that PqMYB2, PqMYB46, and PqMYB72 had correlation coefficients that were higher than 0.8 with PqCHS, PqANS4, and PqCCoAMT10, respectively. Furthermore, a transient expression assay confirmed that the three PqMYBs were localized in the nucleus. We speculated that these three PqMYBs were related to flavonoid biosynthesis in P. quinquefolius. These results provided a theoretical basis and a new perspective for further understanding the R2R3-MYB gene family and the biosynthesis mechanism of secondary metabolites in P. quinquefolius.

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Section: Discussionmentioning

confidence: 99%

Identification and Expression Analysis of R2R3-MYB Transcription Factors Associated with Flavonoid Biosynthesis in Panax quinquefolius

Song,

Yan,

Guo

et al. 2024

IJMS

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“…Through a genome-wide search, Cao et al [ 148 ] identified another two candidate R3 MYB repressors (SlMYBATV-like and SlTRY) and four candidate R2R3 MYB repressors, SlMYB3, SlMYB7, SlMYB32, and SlMYB76. Recently, Zhang et al [ 223 ] found that SlMYB7 expression was positively correlated with the anthocyanin content changes observed in ‘Black Pearl’ fruit, which are purple–green, then become dark purple as they ripen, and finally turn purple–red when fully ripe. Transient overexpression revealed that SlMYB7 acted as a repressor of anthocyanin synthesis by inhibiting the transcription of structural genes of the pathway through direct promoter binding and interacting with the bHLH proteins SlJAF13 and SlAN1, thus precluding the formation of the MBW complex.…”

Section: Tomatomentioning

confidence: 99%

Genetic and Biotechnological Approaches to Improve Fruit Bioactive Content: A Focus on Eggplant and Tomato Anthocyanins

Cammareri,

Frary,

Frary

et al. 2024

IJMS

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Anthocyanins are a large group of water-soluble flavonoid pigments. These specialized metabolites are ubiquitous in the plant kingdom and play an essential role not only in plant reproduction and dispersal but also in responses to biotic and abiotic stresses. Anthocyanins are recognized as important health-promoting and chronic-disease-preventing components in the human diet. Therefore, interest in developing food crops with improved levels and compositions of these important nutraceuticals is growing. This review focuses on work conducted to elucidate the genetic control of the anthocyanin pathway and modulate anthocyanin content in eggplant (Solanum melongena L.) and tomato (Solanum lycopersicum L.), two solanaceous fruit vegetables of worldwide relevance. While anthocyanin levels in eggplant fruit have always been an important quality trait, anthocyanin-based, purple-fruited tomato cultivars are currently a novelty. As detailed in this review, this difference in the anthocyanin content of the cultivated germplasm has largely influenced genetic studies as well as breeding and transgenic approaches to improve the anthocyanin content/profile of these two important solanaceous crops. The information provided should be of help to researchers and breeders in devising strategies to address the increasing consumer demand for nutraceutical foods.

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“…Transcriptional repressors also play a role in the regulation of anthocyanin synthesis. In tomato (Solanum lycopersicum L.), SlMYB7 has been identified to suppress the expression of SlANS [26]. In pear (Pyrus spp.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of Fifteen Gene Families Involved in Anthocyanin Synthesis in Pear

Zhang,

Song,

et al. 2024

Horticulturae

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Anthocyanins play a crucial role in imparting red coloration to pear fruits. However, the specific number and expression patterns of each member within the anthocyanin biosynthesis-related gene families in pears require systematic exploration. In this study, based on the pear genome we identified 15 gene families involved in the anthocyanin biosynthesis pathway using the BLASTP and Hidden Markov Model search methods, comprising a total of 94 enzyme genes. Through phylogenetic analysis, conserved domains, motif, and gene structure analysis, these gene families were further categorized into eight distinct lineages. Subsequent collinearity analysis revealed that the expansion of anthocyanin synthesis-related gene families primarily originated from segmental duplications. Analysis of cis-element in the promoter regions of genes related to anthocyanin synthesis unveiled the presence of light-responsive elements and various hormone-responsive elements. This suggests that changes in light stimulation and hormone levels may influence anthocyanin synthesis. RNA-Seq and qRT-PCR analyses indicated differential expression of anthocyanin biosynthesis-related genes between the peel and flesh tissues. During the accumulation of anthocyanins in red-fleshed pears, upstream genes in the anthocyanin biosynthesis pathway such as PbrPAL2, PbrC4H2, PbrC4H3, Pbr4CL2, Pbr4CL17, PbrF3H5, and PbrF3H6 exhibited high expression levels, likely contributing significantly to the red coloration of pear flesh. In summary, we have identified the number of gene family members involved in pear anthocyanin biosynthesis and analyzed the expression patterns of the genes related to pear anthocyanin biosynthesis. These findings provide a solid foundation for further research on the regulatory mechanisms underlying pear anthocyanin biosynthesis and the breeding of red pear varieties.

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SlMYB7, an AtMYB4-Like R2R3-MYB Transcription Factor, Inhibits Anthocyanin Accumulation in Solanum lycopersicum Fruits

Cited by 8 publications

References 49 publications

Identification and Expression Analysis of R2R3-MYB Transcription Factors Associated with Flavonoid Biosynthesis in Panax quinquefolius

Identification and Expression Analysis of R2R3-MYB Transcription Factors Associated with Flavonoid Biosynthesis in Panax quinquefolius

Genetic and Biotechnological Approaches to Improve Fruit Bioactive Content: A Focus on Eggplant and Tomato Anthocyanins

Genome-Wide Identification and Expression Analysis of Fifteen Gene Families Involved in Anthocyanin Synthesis in Pear

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