Abstract:An olive-green mutant was generated in Synechocystis sp. strain PCC 6803 by inactivation of the sll0396 gene. Whole-cell absorption spectra of the mutant revealed the missing of phycocyanin peak. An investigation of the low-temperature fluorescence emission spectra revealed that the sll0396X mutant has a reduced amount of phycocyanin. Western blot analysis showed that the mutant contained less phycocyanin b-and a-subunits and lacked the 30-and 32-kDa linker polypeptides, and northern blot analysis revealed tha… Show more
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