Slit1 promotes regenerative neurite outgrowth of adult dorsal root ganglion neurons in vitro via binding to the Robo receptor

Changes in expression of Slit1 and its receptor Robo2 in trigeminal ganglion and inferior alveolar nerve following inferior alveolar nerve axotomy in adult rats: a pilot study

“…4 However, increased expression of Slit1 and Robo2 in TGs following IAN axotomy suggests that the expression of Slit1 and Robo2 is primarily influenced by target tissues that induce nerve regeneration 5,8,21 ; additionally, increased expression of Slit1 and Robo2 may also reflect the induction of a nerve regenerative programme by TGs of axotomized rats. 8,15 Our results also showed that Slit1 and Robo2 were highly expressed in axotomized IANs and that low expression levels were observed in non-axotomized IANs (which contained many non-degenerating axons). The changes in expression of Slit1 and Robo2 over time post-axotomy suggest that over-expressed Slit1 in the TGs and IANs may act as a chemoattractant axonal guidance cue for regenerating IAN axons.…”

“…1,[5][6][7][8][9][10] Previous studies have shown that Slit proteins are important guidance cues and that their interaction with Robo receptors plays an important role in neuronal migration, axonal elongation, and axonal branching. 6,8,[11][12][13][14][15][16][17] Although Slit1 and Robo2 are known to be involved in neuronal development at various early embryonic stages, whether Slit1 and its receptor Robo2 play a role in regeneration and functional recovery of the adult injured IAN following axotomy is unknown.…”

Slit–Robo GTPase‐Activating Proteins are Differentially Expressed in Murine Dorsal Root Ganglia: Modulation by Peripheral Nerve Injury

“…Our previous study showed that Robo1 and Robo2 are both expressed in adult rat DRGs, and nerve injury can induce upregulation of these proteins at this location (Yi et al,2006). Moreover, our in vitro study suggests that Robo2 may promote the outgrowth of neurites in cultured primary DRG neurons (Zhang et al,2010). However, it remains unclear whether and how members of the srGAPs might be involved in DRG neural regeneration.…”

Axon regeneration in C. elegans: Worming our way to mechanisms of axon regeneration

“…With its strong knowledge base and robust research community, as well as its particular experimental advantages discussed above, C. elegans is positioned to make important contributions. Conservation of function between molecules that regulate axon regeneration in C. elegans and those that regulate axon regeneration in mammals, including DLK and PTEN, along with the conserved roles and regulators of calcium signaling, microtubule dynamics, axon guidance, and neuronal aging in axon regeneration, suggest findings in C. elegans will inform our understanding of mammalian axon regeneration (Neumann et al, 2002; Filbin, 2003; Raivich et al, 2004; Spencer and Filbin, 2004; Benson et al, 2005; Erturk et al, 2007; Fabes et al, 2007; Wu et al, 2007; Gabel et al, 2008; Liu et al, 2008; Low et al, 2008; Hammarlund et al, 2009; Itoh et al, 2009; Montolio et al, 2009; Yan et al, 2009; Ghosh-Roy et al, 2010; Giger et al, 2010; Zhang et al, 2010; Chen et al, 2011; Nix et al, 2011; Ghosh-Roy et al, 2012; Shin et al, 2012; Watkins et al, 2013; Byrne et al, 2014; Tang and Chisholm, 2016). …”

“…For example, as in the mammalian nervous system, axon guidance pathways including netrin, ephrin, WNT, and SLT/ROBO play an important role in the ability of severed axons to regenerate towards their target (Benson et al, 2005; Fabes et al, 2007; Wu et al, 2007; Gabel et al, 2008; Liu et al, 2008; Low et al, 2008; Giger et al, 2010; Zhang et al, 2010; Chen et al, 2011). C. elegans lacks some environmental features of mammalian axon regeneration, such as myelin, chondroitin sulfate proteoglycans, and macrophages.…”