Slit Is the Midline Repellent for the Robo Receptor in Drosophila

Kidd, Thomas; Bland, Kimberly S.; Goodman, Corey S.

doi:10.1016/s0092-8674(00)80589-9

Cited by 879 publications

(761 citation statements)

References 26 publications

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“…For example, it occurs when Drosophila commissural neurons cross the mid-line: these growth cones begin expressing ROBO on their surface only after contacting COMM-expressing cells (Kidd et al, 1999;Keleman et al, 2005). C. elegans growth cones are also reprogrammed, for example, when the hermaphrodite-specific neurons HSNL encounter guidepost cells in the vulval epithelium, a process that requires the guidepost protein SYG-2 and its receptor SYG-1 (Shen et al, 2004).…”

Section: Time-delayed Guidepost Cellsmentioning

confidence: 99%

Fishing lines, time‐delayed guideposts, and other tricks used by developing pharyngeal neurons in Caenorhabditis elegans

Pilon

2008

Developmental Dynamics

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The 20 neurons that innervate the Caenorhabditis elegans pharynx form a simple nervous system that develops and operates in near complete isolation from the rest of the worm body and, therefore, offers a manageable degree of complexity for developmental genetics studies. This review discusses the progress that has been made in determining the mechanisms by which 4 of the 20 pharyngeal neurons develop, and emphasizes surprising processes that add to the classic growth cone guidance model which is usually thought to explain how most axons establish their trajectories.

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Section: Time-delayed Guidepost Cellsmentioning

confidence: 99%

Fishing lines, time‐delayed guideposts, and other tricks used by developing pharyngeal neurons in Caenorhabditis elegans

Pilon

2008

Developmental Dynamics

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“…In robo mutants, axons freely cross and recross the midline, whereas in slit mutants, axons enter, but never leave, the midline. Thus, in the absence of Robo, another receptor must respond to Slit to ensure that growth cones/axons do not linger at the midline, without interfering with their ability to cross the midline (see Kidd et al, 1999). A good candidate for this additional Slit receptor is Robo2, a close relative of Robo that is expressed on developing CNS neurons.…”

Section: Drosophila Ventral Nerve Cordmentioning

confidence: 99%

“…A good candidate for this additional Slit receptor is Robo2, a close relative of Robo that is expressed on developing CNS neurons. A straightforward prediction of this model is that the phenotype of robo/robo2 double mutants should resemble the phenotype of slit single mutants (see Kidd et al, 1999).…”

Section: Drosophila Ventral Nerve Cordmentioning

confidence: 99%

Axon guidance at the midline choice point

Kaprielian

Runko

Imondi

2001

Developmental Dynamics

145

108

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The central nervous system (CNS) of higher organisms is bilaterally-symmetric. The transfer of information between the two sides of the nervous system occurs through commissures formed by neurons that project axons across the midline to the contralateral side of the CNS. Interestingly, these axons cross the midline only once. Other neurons extend axons that never cross the midline; they project exclusively on their own (ipsilateral) side of the CNS. Thus, the midline is an important choice point for several classes of pathfinding axons. Recent studies demonstrate that specialized midline cells play critical roles in regulating the guidance of both crossing and non-crossing axons at the ventral midline of the developing vertebrate spinal cord and the Drosophila ventral nerve cord. For example, these cells secrete attractive cues that guide commissural axons over long distances to the midline of the CNS. Furthermore, shortrange interactions between guidance cues present on the surfaces of midline cells, and their receptors expressed on the surfaces of pathfinding axons, allow commissural axons to cross the midline only once and prevent ipsilaterally-projecting axons from entering the midline. Remarkably, the molecular composition of commissural axon surfaces is dynamically-altered as they cross the midline. Consequently, commissural axons become responsive to repulsive midline guidance cues that they had previously ignored on the ipsilateral side of the midline. Concomitantly, commissural axons lose responsiveness to attractive guidance cues that had initially attracted them to the midline. Thus, these exquisitely regulated guidance systems prevent commissural axons from lingering within the confines of the midline and allow them to pioneer an appropriate pathway on the contralateral side of the CNS. Many aspects of midline guidance are controlled by mechanistically and evolutionarily-conserved ligand-receptor systems. Strikingly, recent studies demonstrate that these receptors are modular; the ectodomains determine ligand recognition and the cytoplasmic domains specify the response of an axon to a given guidance cue. Despite rapid and dramatic progress in elucidating the molecular mechanisms that control midline guidance, many questions remain.

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“…During early stages of nerve cord development, two classes of neurons can be distinguished based on whether or not their axons cross the midline. The pathfinding of these axons are directed by cues from the midline cells, which simultaneously express both attractive guidance cues such as Netrin Mitchel et al, 1996) and repulsive cues such as Slit and Semaphorin proteins (Rothberg et al, 1990;Kidd et al, 1999;Brose et al, 1999;Y. Zou et al, 2000).…”

Section: Introductionmentioning

confidence: 99%