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Liu, Yehe; Levenson, Richard M.; Jenkins, Michael W.

doi:10.1016/j.ajpath.2021.10.010

Cited by 14 publications

(2 citation statements)

References 94 publications

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“…Non-invasive, slide-free imaging procedures may be helpful in avoiding artifacts and tissue damage. The application of such techniques is an important step forward in digital pathology [31]. In human examinations, 3D ultrasound imaging can already be regarded as a state-ofthe-art technique [32].…”

Section: Plos Onementioning

confidence: 99%

A workflow to visualize vertebrate eyes in 3D

Wang,

Baumgarten,

Balcewicz

et al. 2023

PLoS ONE

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Purpose To establish a workflow to visualize the surgical anatomy in 3D based on histological data of eyes of experimental animals for improving the planning of complex surgical procedures. Methods Four C57BL/6J wild-type(wt) mouse eyes, three Brown Norway rat eyes and four Chinchilla Bastard rabbit eyes were enucleated and processed for standard histology with serial sections and hematoxylin and eosin staining. Image stacks were processed to obtain a representation of the eye anatomy in 3D. In addition, virtual image stacks and 3D point clouds were generated by processing sagittal sections of eyes with stepwise 180° rotation and projection around the eye axis to construct a rotationally symmetric 3D model from one single sagittal section. Results Serial sections of whole eyes of mice, rats and rabbits showed significant artifacts interfering with a practical image stack generation and straightforward 3D reconstruction despite the application of image registration techniques. A workflow was established to obtain a 3D image of the eye based on virtual image stacks and point cloud generation by rotation of a single sagittal section of the eye around the symmetry axis. By analyzing the tissue shrinkage during histological processing true biometric reconstructions of the eyes were feasible making the resulting model usable for 3D modeling and simulation, e.g. for planning of complex surgical procedures in different species. Conclusion Because serial sections of the eye with standard histological protocols yielded too many artifacts for a straightforward 3D visualization we reconstructed a pseudorealistic 3D model based on virtual image stacks and point cloud generation calculated from a single sagittal section of the eye. Such a model detailing microscopic structures of the whole eye will allow for a specific planning of surgical procedures in small animal eyes in order to prevent surgical complications in a very early stage of an experiment and it will support the design and development of complex intraocular implants. It will therefore be helpful in surgical teaching and improve laboratory animal welfare by an expected reduction of experimental animal numbers. Further processing including integration of mechanical tissue properties is needed to convert these 3D models into a practical virtual reality teaching and simulation platform for eyes of several species.

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Section: Plos Onementioning

confidence: 99%

A workflow to visualize vertebrate eyes in 3D

Wang,

Baumgarten,

Balcewicz

et al. 2023

PLoS ONE

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“…Параллельно развитию сферы автоматизации лабораторной подготовки патологоанатомических препаратов наблюдается появление новых методов патологоанатомической диагностики, которые ведут к отказу в использовании гистологических стекол, так называемые slide-free методы микроскопии [68]. Данный подход позволяет значительно снизить время работы с материалом, поскольку из многоступенчатой подготовки исключается фиксация или заморозка препарата, а окраска микропрепарата становится опциональной, так как самой важной частью становится качественная визуализация структур с помощью высокоразрешающих камер.…”

Section: пробоподготовкаunclassified

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2022

RJTEH

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НАУЧНАЯ СПЕЦИАЛИЗАЦИЯ -электронное здравоохранение, телемедицина, медицинская информатика и кибернетика, мобильное здоровье, организация здравоохранения, дистанционное обучение, страховая медицинская телематика, медицинская аппаратура, биомедицинская инженерия, биоинформатика.АУДИТОРИЯ -врачи всех специальностей, главные врачи ЛПУ, руководители IT-отделов ЛПУ, инженеры и разработчики медицинской техники и медицинского оборудования, руководители и сотрудники информационно-аналитических центров.

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Disrupting prostate cancer research: Challenge accepted; report from the 2023 Coffey‐Holden Prostate Cancer Academy Meeting

Miyahira,

Kamran,

Jamaspishvili

et al. 2024

The Prostate

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IntroductionThe 2023 Coffey‐Holden Prostate Cancer Academy (CHPCA) Meeting, themed “Disrupting Prostate Cancer Research: Challenge Accepted,” was convened at the University of California, Los Angeles, Luskin Conference Center, in Los Angeles, CA, from June 22 to 25, 2023.MethodsThe 2023 marked the 10th Annual CHPCA Meeting, a discussion‐oriented scientific think‐tank conference convened annually by the Prostate Cancer Foundation, which centers on innovative and emerging research topics deemed pivotal for advancing critical unmet needs in prostate cancer research and clinical care. The 2023 CHPCA Meeting was attended by 81 academic investigators and included 40 talks across 8 sessions.ResultsThe central topic areas covered at the meeting included: targeting transcription factor neo‐enhancesomes in cancer, AR as a pro‐differentiation and oncogenic transcription factor, why few are cured with androgen deprivation therapy and how to change dogma to cure metastatic prostate cancer without castration, reducing prostate cancer morbidity and mortality with genetics, opportunities for radiation to enhance therapeutic benefit in oligometastatic prostate cancer, novel immunotherapeutic approaches, and the new era of artificial intelligence‐driven precision medicine.DiscussionThis article provides an overview of the scientific presentations delivered at the 2023 CHPCA Meeting, such that this knowledge can help in facilitating the advancement of prostate cancer research worldwide.

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Cited by 14 publications

References 94 publications

A workflow to visualize vertebrate eyes in 3D

A workflow to visualize vertebrate eyes in 3D

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Disrupting prostate cancer research: Challenge accepted; report from the 2023 Coffey‐Holden Prostate Cancer Academy Meeting

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