Sleeve gastrectomy and pancreas omentoplasty improved β cell insulin expression and interleukin-1β serum level in non-obese diabetes mellitus rat

Abstract

Background: Diabetes mellitus is one of the health problems that is growing rapidly today. In Asia, majority of the population are non-obese with Type 2 Diabetes Mellitus (T2DM). Two main defects of T2DM, insulin resistance and impaired insulin secretion due to reduced pancreatic β cells. Persistent metabolic stress due to hyperglycemic conditions, causes inflammation of the islets of Langerhans. Interleukin-1β (IL-1β) signaling is the most frequent pathway inducing β cell dysfunction. The bariatric Sleeve Gastrectomy (SG) procedure increases particular hormone which stimulates β cell growth and pancreatic insulin production. Aside from the role as a blood glucose regulator, insulin also plays a role in anti-inflammatory modulation. Omentoplasty can be used to wrap organ or tissue structures. The Omentum has unique biological functions, including promote tissue regeneration and anti-inflammatory effect. The study evaluates the effect of Sleeve Gastrectomy and pancreas Omentoplasty procedures on β cell insulin expression and Interleukin-1β (IL-1β) serum levels in non-obese diabetes mellitus subjects.Methods: Experimental study with "post-test control group design" on 27 rats with Diabetes Mellitus was divided into 3 groups: K (control), P1 (Sleeve Gastrectomy), P2 (Sleeve Gastrectomy + Omentoplasty). 10 days after procedure, we evaluated the insulin expression of β cells using monoclonal antibody anti-insulin stain and IL-1β serum levels using rat IL-1β Enzyme-Linked Immunosorbent Assay (ELISA) kit. Statistical analysis with Kruskal Wallis and Mann Whitney test. Correlation test with Spearman.Results: β cell insulin expression increased in P2 (p=0.020), compared to control. While the IL-1β serum levels reduced significantly in all groups (p<0.001) compared to control. Moderate negative correlation (r = -0.476) between β cell insulin expression and IL-1β serum levels.Conclusion: Sleeve gastrectomy and omentoplasty increased β cell insulin expression and decreased IL-1 β serum levels in non-obese rats with diabetes mellitus.

Sleeve Gastrectomy Decrease Body Weight, Fasting Blood Glucose, and Gene Expression of TNF-α and IL-1 in The Abdominal Aorta of Rats with Obesity and Diabetes Mellitus

“…(14) Previous studies also suggests that SG procedure had the same effect on TNF-α and IL-1 as other common bariatric procedures and when combined with other techniques. (15,16) Hence, it could be assumed that SG could produce better medical outcomes for the patient, however the association with TNF-α and IL-1 has not been elucidated well enough. Therefore, this study was aimed to determine the weight loss, reduced fasting blood glucose (FBG), as well as the decrease of TNF-α and IL-1 gene expression in the abdominal aorta of obese and DM rats undergoing SG procedures.…”

“…The Indonesian Biomedical Journal, Vol 15,. No.4, August 2023, p.296-368 Print ISSN: 2085-3297, Online ISSN: 2355-9179…”

Sleeve Gastrectomy and Liver Omentoplasty Can Increased Il-10 and TGF-Β Expression on Liver Fibrosis in Obesity

“…Until now, the management of liver disease is still focused on treating the primary disease; the only rational treatment for liver fibrosis is liver transplantation. Liver transplantation was clearly stated as the sole option of treatment when body having a liver failure [3]. While the success rate data were stated that liver transplantation results are more inferior than kidney transplantation, only 70-80% of recipients survive for at least 1 year.…”