Sleeping Sickness at the Crossroads

Burri, Christian

doi:10.3390/tropicalmed5020057

Cited by 8 publications

(7 citation statements)

References 38 publications

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“…Bei klinisch schweren Formen wird immer noch eine Lumbalpunktion durchgeführt und bei pathologischem Liquor die Kombinationstherapie Nifurtimox per os / Eflorni thin intravenös angewendet [2]. Die perorale Behandlung kann nun auch in nicht optimal eingerichteten Gesundheits zentren in der Peripherie durchgeführt werden [3]. Durch diese Anstrengungen gingen die Schlafkrankheitsfälle wieder deut lich zurück.…”

Section: Die Krankheitunclassified

Schlafkrankheit: der gefährliche Schlaf nach dem Erfolg

et al. 2023

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Section: Die Krankheitunclassified

Schlafkrankheit: der gefährliche Schlaf nach dem Erfolg

et al. 2023

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“…However, a similar situation was already achieved in the 1960s, which was followed by a reduction in surveillance and control activities and as a consequence the disease resurfaced again in the 1990s (Brun et al, 2010). This advocates that efforts to develop trypanosomespecific diagnostics and therapeutics should not be suspended (Burri, 2020). Five drugs are currently used in the treatment of HAT (Büscher et al, 2017).…”

Section: Introductionmentioning

confidence: 96%

Analyzing editosome function in high-throughput

Campo

Leeder

Reissig³

et al. 2020

Preprint

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Mitochondrial gene expression in African trypanosomes and other trypanosomatid pathogens requires a U-nucleotide specific insertion/deletion-type RNA-editing reaction. The process is catalyzed by a macromolecular protein complex known as the editosome. Editosomes are restricted to the trypanosomatid clade and since editing is essential for the parasites, the protein complex represents a near perfect target for drug intervention strategies. Here we report the development of an improved in vitro assay to monitor editosome function. The test system utilizes fluorophore-labeled substrate RNAs to analyze the processing reaction by automated, high-throughput capillary electrophoresis (CE) in combination with a laser-induced fluorescence (LIF) readout. We optimized the assay for high-throughput screening (HTS)-experiments and devised a multiplex fluorophore-labeling regime to scrutinize the U-insertion/U-deletion reaction simultaneously. The assay is robust, it requires only nanogram amounts of materials and it meets all performance criteria for HTS-methods. As such the test system should be helpful in the search for trypanosome-specific pharmaceuticals.

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“…However, a similar situation was already achieved in the 1960s, which was followed by a reduction in surveillance and control activities and as a consequence the disease resurfaced again in the 1990s ( 3 ). This advocates that efforts to develop trypanosome-specific diagnostics and therapeutics should not be suspended ( 4 ). Five drugs are currently used in the treatment of HAT ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

Analyzing editosome function in high-throughput

Campo

Leeder

Reißig

et al. 2020

Nucleic Acids Research

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Mitochondrial gene expression in African trypanosomes and other trypanosomatid pathogens requires a U-nucleotide specific insertion/deletion-type RNA-editing reaction. The process is catalyzed by a macromolecular protein complex known as the editosome. Editosomes are restricted to the trypanosomatid clade and since editing is essential for the parasites, the protein complex represents a near perfect target for drug intervention strategies. Here, we report the development of an improved in vitro assay to monitor editosome function. The test system utilizes fluorophore-labeled substrate RNAs to analyze the processing reaction by automated, high-throughput capillary electrophoresis (CE) in combination with a laser-induced fluorescence (LIF) readout. We optimized the assay for high-throughput screening (HTS)-experiments and devised a multiplex fluorophore-labeling regime to scrutinize the U-insertion/U-deletion reaction simultaneously. The assay is robust, it requires only nanogram amounts of materials and it meets all performance criteria for HTS-methods. As such the test system should be helpful in the search for trypanosome-specific pharmaceuticals.

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Sleeping Sickness at the Crossroads

Abstract: Human African trypanosomiasis (HAT; sleeping sickness) is a disease with truly historic dimensions [...]

Cited by 8 publications

References 38 publications

Schlafkrankheit: der gefährliche Schlaf nach dem Erfolg

Schlafkrankheit: der gefährliche Schlaf nach dem Erfolg

Analyzing editosome function in high-throughput

Analyzing editosome function in high-throughput

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