Sleep deprivation negatively impacts reproductive output in <i>Drosophila melanogaster</i>

Potdar, Sheetal; Daniel, Danita K.; Thomas, Femi Anna; Lall, Shraddha; Sheeba, Vasu

doi:10.1101/158071

Cited by 11 publications

(11 citation statements)

References 35 publications

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“…Sleep deprivation can have a negative influence on attention, motivation, memory (Rolls et al, 2011; Vyazovskiy et al, 2011), and reproductive output (Potdar et al, 2018). Note that females with little or no help from their partner can hardly compensate for the lack of sleep during their off-nest time, because they are off-nest for only 10% to 15% of the time, during which they need to forage.…”

Section: Discussionmentioning

confidence: 99%

Daily Rhythms of Female Self-maintenance Correlate with Predation Risk and Male Nest Attendance in a Biparental Wader

et al. 2020

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Parents make tradeoffs between care for offspring and themselves. Such a tradeoff should be reduced in biparental species, when both parents provide parental care. However, in some biparental species, the contribution of one sex varies greatly over time or between pairs. How this variation in parental care influences self-maintenance rhythms is often unclear. In this study, we used continuous video recording to investigate the daily rhythms of sleep and feather preening in incubating females of the Northern Lapwing ( Vanellus vanellus), a wader with a highly variable male contribution to incubation. We found that the female’s sleep frequency peaked after sunrise and before sunset but was low in the middle of the day and especially during the night. In contrast, preening frequency followed a 24-h rhythm and peaked in the middle of the day. Taken together, incubating females rarely slept or preened during the night, when the predation pressure was highest. Moreover, the sleeping and preening rhythms were modulated by the male contribution to incubation. Females that were paired with more contributing males showed a stronger sleep rhythm but also a weaker preening rhythm. If more incubating males also invest more in nest guarding and deterring daylight predators, their females may afford more sleep on the nest during the day and preen more when they are off the nest. Whether the lack of sleep in females paired with less caregiving males has fitness consequences awaits future investigation.

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Section: Discussionmentioning

confidence: 99%

Daily Rhythms of Female Self-maintenance Correlate with Predation Risk and Male Nest Attendance in a Biparental Wader

et al. 2020

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“…Given the potential for Wolbachia to influence fitness estimates, and potentially to create signals of intralocus conflict, what should we do about it? Reproductive success is the primary sex‐specific measure of fitness (e.g., Baker et al, ; Nguyen & Moehring, ; Punzalan et al, ; Potdar, Daniel, Thomas, Lall, & Sheeba, ; Sharp & Agrawal, ; Tobler, Hermisson, & Schlötterer, ; Travers, Garcia‐Gonzalez, & Simmons, ), but in a range of invertebrates, sexual fitness might be affected by Wolbachia . This is particularly true for Drosophila species given that in 2005 it was reported that ca.…”

Section: Discussionmentioning

confidence: 99%

Wolbachia infection can bias estimates of intralocus sexual conflict

Duffy

Archer

Sharma

et al. 2018

Ecology and Evolution

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Males and females share most of their genome and develop many of the same traits. However, each sex frequently has different optimal values for these shared traits, creating intralocus sexual conflict. This conflict has been observed in wild and laboratory populations of insects and affects important evolutionary processes such as sexual selection, the maintenance of genetic variation, and possibly even speciation. Given the broad impacts of intralocus conflict, accurately detecting and measuring it is important. A common way to detect intralocus sexual conflict is to calculate the intersexual genetic correlation for fitness, with negative values suggesting conflict. Here, we highlight a potential confounder of this measure—cytoplasmic incompatibility caused by the intracellular parasite Wolbachia. Infection with Wolbachia can generate negative intersexual genetic correlations for fitness in insects, suggestive of intralocus sexual conflict. This is because cytoplasmic incompatibility reduces the fitness of uninfected females mated to infected males, while uninfected males will not suffer reductions in fitness if they mate with infected females and may even be fitter than infected males. This can lead to strong negative intersexual genetic correlations for fitness, mimicking intralocus conflict. We illustrate this issue using simulations and then present Drosophila simulans data that show how reproductive incompatibilities caused by Wolbachia infection can generate signals of intralocus sexual conflict. Given that Wolbachia infection in insect populations is pervasive, but populations usually contain both infected and uninfected individuals providing scope for cytoplasmic incompatibility, this is an important consideration for sexual conflict research but one which, to date, has been largely underappreciated.

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“…Caffeine was previously reported to decrease sleep in female fmn flies 66 , raising the possibility that fmn fly locomotion may reciprocally increase in response to caffeine. To test for this possibility, we measure locomotion in response to caffeine feeding (0.5mg/ml) in male flies.…”

Section: Ric Activity Impacts Amphetamine Sensitivity In a Dat-dependent Mannermentioning

confidence: 98%

Dopaminergic Ric GTPase activity impacts amphetamine sensitivity and sleep quality in a dopamine transporter-dependent manner in Drosophila melanogaster

Fagan¹,

Kearney²,

Luethi

et al. 2021

Preprint

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Dopamine (DA) is required for movement, sleep, and reward, and DA signaling is tightly controlled by the presynaptic DA transporter (DAT). Therapeutic and addictive psychostimulants, including methylphenidate (Ritalin; MPH), cocaine, and amphetamine (AMPH), markedly elevate extracellular DA via their actions as competitive DAT inhibitors (MPH, cocaine) and substrates (AMPH). DAT silencing in mice and invertebrates results in hyperactivity, reduced sleep, and blunted psychostimulant responses, highlighting DAT′s essential role in DA-dependent behaviors. DAT surface expression is not static; rather it is dynamically regulated by endocytic trafficking. PKC-stimulated DAT endocytosis requires the neuronal GTPase, Rit2, and Rit2 silencing in mouse DA neurons impacts psychostimulant sensitivity. However, it is unknown whether or not Rit2-mediated changes in psychostimulant sensitivity are DAT-dependent. Here, we leveraged Drosophila melanogaster to test whether the Drosophila Rit2 ortholog, Ric, impacts dDAT function, trafficking, and DA-dependent behaviors. Orthologous to hDAT and Rit2, dDAT and Ric directly interact, and the constitutively active Ric mutant Q117L increased dDAT surface levels and function in cell lines and ex vivo Drosophila brains. Moreover, DAergic RicQ117L expression caused sleep fragmentation in a DAT-dependent manner, but had no effect on total sleep and daily locomotor activity. Importantly, we found that Rit2 is required for AMPH-stimulated DAT internalization in mouse striatum, and that DAergic RicQ117L expression significantly increased Drosophila AMPH sensitivity in a DAT-dependent manner, suggesting a conserved impact of Ric-dependent DAT trafficking on AMPH sensitivity. These studies support that the DAT/Rit2 interaction impacts both baseline behaviors and AMPH sensitivity, potentially by regulating DAT trafficking.

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Sleep deprivation negatively impacts reproductive output in Drosophila melanogaster

Cited by 11 publications

References 35 publications

Daily Rhythms of Female Self-maintenance Correlate with Predation Risk and Male Nest Attendance in a Biparental Wader

Daily Rhythms of Female Self-maintenance Correlate with Predation Risk and Male Nest Attendance in a Biparental Wader

Wolbachia infection can bias estimates of intralocus sexual conflict

Dopaminergic Ric GTPase activity impacts amphetamine sensitivity and sleep quality in a dopamine transporter-dependent manner in Drosophila melanogaster

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