Sleep deprivation negatively impacts reproductive output in <i>Drosophila melanogaster</i>

Potdar, Sheetal; Daniel, Danita K.; Thomas, Femi Anna; Lall, Shraddha; Sheeba, Vasu

doi:10.1242/jeb.174771

Cited by 18 publications

(9 citation statements)

References 43 publications

(47 reference statements)

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“…To assess behavioral consequences of consolidating sleep in aged flies, we examined reproductive fitness following sleep opportunity restriction. Aged female flies normally exhibit a dramatic reduction in reproductive output 70 , and reproductive output is also impaired with sleep deprivation 71 . We tested whether such decrements are modifiable with improved sleep.…”

Section: Resultsmentioning

confidence: 99%

A Drosophila model of sleep restriction therapy for insomnia

et al. 2019

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Insomnia is the most common sleep disorder among adults, especially affecting individuals of advanced age or with neurodegenerative disease. Insomnia is also a common comorbidity across psychiatric disorders. Cognitive Behavioral Therapy for Insomnia (CBT-I) is the first-line treatment for insomnia; a key component of this intervention is restriction of sleep opportunity, which optimizes matching of sleep ability and opportunity, leading to enhanced sleep drive. Despite the well-documented efficacy of CBT-I, little is known regarding how CBT-I works at a cellular and molecular level to improve sleep, due in large part to an absence of experimentally-tractable animals models of this intervention. Here, guided by human behavioral sleep therapies, we developed a Drosophila model for Sleep Restriction Therapy (SRT) of insomnia. We demonstrate that restriction of sleep opportunity through manipulation of environmental cues improves sleep efficiency in multiple short-sleeping Drosophila mutants. The response to sleep opportunity restriction requires ongoing environmental inputs, but is independent of the molecular circadian clock. We apply this sleep opportunity restriction paradigm to aging and Alzheimer’s Disease fly models, and find that sleep impairments in these models are reversible with sleep restriction, with associated improvement in reproductive fitness and extended lifespan. This work establishes a model to investigate the neurobiological basis of CBT-I, and provides a platform that can be exploited towards novel treatment targets for insomnia.

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Section: Resultsmentioning

confidence: 99%

A Drosophila model of sleep restriction therapy for insomnia

et al. 2019

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“…Both increased total sleep and increased sleep consolidation suggest that improved sleep quality could aid in mitigating alcohol-induced pathologies. Although there have been few studies examining the relationship between sleep health and alcohol toxicity, sleep loss or decreased sleep consolidation has been shown to reduce reproductive output, accelerate aging and increase the accumulation of reactive oxygen species and death in flies 154,155 . In humans, increasing sleep in adolescents is correlated with decreased risk of emotional and cognitive disruption as well as lowered risk of obesity 156 .…”

Section: Sleep Disorders and Sleep Disturbances Have Become Increasingly Prevalent In Modern Societymentioning

confidence: 99%

Sleep modulates alcohol toxicity inDrosophila

Noakes

et al. 2021

Preprint

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Study Objectives: Alcohol abuse is a significant public health problem, particularly in populations in which sleep deprivation is common as such as shift workers and aged individuals. Although research demonstrates the effect of alcohol on sleep, little is known about the role of sleep in alcohol sensitivity and toxicity. We investigated sleep as a factor modulating alcohol toxicity using Drosophila melanogaster, a model system ideal for studies of sleep, alcohol and aging. Methods: Following 24 hours of sleep deprivation using mechanical stimulation, Drosophila were exposed to binge-like alcohol exposures. Behavioral sensitivity, tolerance, and mortality were assessed. The effects of chronic sleep deprivation on alcohol toxicity were investigated using a short sleep mutant insomniac. Pharmacological induction of sleep for prior to alcohol exposure was accomplished using a GABAA-receptor agonist, 4,5,6,7-tetrahydroisoxazolo(5,4-c)pyridin-3-ol (THIP) to determine if increased sleep mitigated the effects of alcohol toxicity on middle-aged flies and flies with environmentally disrupted circadian clocks mimicking groups more vulnerable to the effects of alcohol. Results: Acute sleep deprivation increased alcohol-induced mortality following alcohol exposure. However, sleep deprivation had no effect on alcohol absorbance or clearance. Sleep deprivation also abolished functional tolerance measured 24 hours after the initial alcohol exposure, although tolerance at 4 h was observed. Pharmacologically increasing sleep prior to alcohol exposure decreased alcohol-induced mortality. Conclusions: Sleep quantity prior to alcohol exposure affects alcohol toxicity with decreased sleep increasing alcohol toxicity and dampened 24-hour alcohol tolerance. In contrast, increased sleep mitigated alcohol-induced mortality even in vulnerable groups such as aging flies and those with circadian dysfunction.

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“…The fact that different reports fail to show sleep recovery after the behavioural switch would favour the second scenario. However, a component of the measurable sleep displayed by mated females confers a fitness benefit since sleep deprived mated females have reduced fecundity (Potdar et al, 2018). We believe that studying the behavioural switch after mating and the characteristics of sleep in mated females, which is likely the default state in wild fruit flies (Giardina et al, 2017), may be critical to understanding the regulation and functions of sleep.…”

Section: Social Interactions Have a Profound Effect On Sleep Regulationmentioning

confidence: 99%

“…It is also critical for development (Kayser and Biron, 2016) and immune function (Besedovsky et al, 2019). In insects, sleep also has an impact on fitness affecting reproductive output (Potdar et al, 2018), susceptibility to acute oxidative stress (Hill et al, 2018), and development (Kayser and Biron, 2016), and is important for learning and memory recall (Beyaert et al, 2012) and synaptic homeostasis (Gilestro et al, 2009; Bushey et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Being essentially offline means that animals are unable to engage in other essential activities, such as foraging or mating. This is evident in the strategies employed by different species in their attempts to balance their need for sleep and remain safe (Rattenborg et al, 1999; Voirin et al, 2014; Tisdale et al, 2018), fed (Willie et al, 2001; Keene et al, 2010), or reproductively successful (Lesku et al, 2012; Potdar et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Sleep in Drosophila and Its Context

Beckwith

French

2019

Front. Physiol.

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A prominent idea emerging from the study of sleep is that this key behavioural state is regulated in a complex fashion by ecologically and physiologically relevant environmental factors. This concept implies that sleep, as a behaviour, is plastic and can be regulated by external agents and changes in internal state. Drosophila melanogaster constitutes a resourceful model system to study behaviour. In the year 2000, the utility of the fly to study sleep was realised, and has since extensively contributed to this exciting field. At the centre of this review, we will discuss studies showing that temperature, food availability/quality, and interactions with conspecifics can regulate sleep. Indeed the relationship can be reciprocal and sleep perturbation can also affect feeding and social interaction. In particular, different environmental temperatures as well as gradual changes in temperature regulate when, and how much flies sleep. Moreover, the satiation/starvation status of an individual dictates the balance between sleep and foraging. Nutritional composition of diet also has a direct impact on sleep amount and its fragmentation. Likewise, aggression between males, courtship, sexual arousal, mating, and interactions within large groups of animals has an acute and long-lasting effect on sleep amount and quality. Importantly, the genes and neuronal circuits that relay information about the external environment and internal state to sleep centres are starting to be elucidated in the fly and are the focus of this review. In conclusion, sleep, as with most behaviours, needs the full commitment of the individual, preventing participation in other vital activities. A vast array of behaviours that are modulated by external and internal factors compete with the need to sleep and thus have a significant role in regulating it.

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Sleep deprivation negatively impacts reproductive output in Drosophila melanogaster

Cited by 18 publications

References 43 publications

A Drosophila model of sleep restriction therapy for insomnia

A Drosophila model of sleep restriction therapy for insomnia

Sleep modulates alcohol toxicity inDrosophila

Sleep in Drosophila and Its Context

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