Sleep deprivation effects on object discrimination task in zebrafish (Danio rerio)

Pinheiro-da-Silva, Jaquelinne; Silva, Priscila Fernandes; Nogueira, Marco Aurélio; Luchiari, Ana Carolina

doi:10.1007/s10071-016-1034-x

Cited by 54 publications

(32 citation statements)

References 72 publications

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“…In this view, our data corroborate other studies showing the zebrafish ability to learn about objects and use the previous acquired information to discriminate a novel environmental feature (Oliveira et al, 2015; Santos et al, 2016; Pinheiro-da-Silva et al, 2017). The ability to learn and change behavior according to one’s experiences is highly valuable to all animal species, as it provides several benefits to fitness.…”

Section: Discussionsupporting

confidence: 91%

“…The behavior recorded in video was tracked through the software ZebTrack (v2.3; Pinheiro-da-Silva et al, 2017) on the MATLAB platform (R2013A 8.1.0.604). The parameters analyzed were: average swimming speed, maximum swimming speed, total distance traveled, freezing and time exploring each object.…”

Section: Methodsmentioning

confidence: 99%

“…The parameters analyzed were: average swimming speed, maximum swimming speed, total distance traveled, freezing and time exploring each object. We considered the animal’s residence time in a 3-cm area around the objects to define exploration behavior (Lucon-Xiccato and Dadda, 2014; Pinheiro-da-Silva et al, 2017). …”

Section: Methodsmentioning

confidence: 99%

“…The HDI reduces uncertainties, indicating values with greater credibility and covering 95% of the data distribution. For our data, the comparison value was set at about 0, and the Region of Practical Equivalence—ROPE was defined at ±5 (Pinheiro-da-Silva et al, 2017). …”

Section: Methodsmentioning

confidence: 99%

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It’s Tea Time: Interference of Ayahuasca Brew on Discriminative Learning in Zebrafish

Lobão-Soares

Eduardo-da-Silva

Amarilha

et al. 2018

Front. Behav. Neurosci.

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Ayahuasca is a psychoactive brew traditionally used in shamanistic and vegetalistic rituals and has recently received lot of attention due to potential cognitive benefits. Ayahuasca effects are caused by the synergistic interaction of β-carbolines (harmine, harmaline and tetrahydroarmine) contained in Banisteriopsis caapi stalks combined with the N,N-dimethyltryptamine (DMT) from Psychotria viridis leaves, a potent agonist to serotonin (5-HT) receptors. The present study approaches the effects of chronic and acute exposure to two Ayahuasca concentrations (0.1 and 0.5 ml/L) on the cognitive ability to discriminate objects in a one-trial learning task in zebrafish. Based on the combination of concentrations and exposure regimens, we divided adult zebrafish in five treatment groups: acute 0.1 and 0.5 ml/L, chronic 0.1 and 0.5 ml/L, and control 0.0 (n = 20 for each group). Then we tested them in a memory task of object discrimination. Acute Ayahuasca exposed groups performed similarly to the control group, however chronically treated fish (13 days) presented both impaired discriminative performance and locomotor alterations. Overall, these results indicate that Ayahuasca is a potent psychoactive drug that, in chronic exposure, negatively affects mnemonic parameters in zebrafish. In single exposure it does not affects cognitive performance, but the higher concentration (0.5) affected locomotion. Moreover, we reinforce the importance of the zebrafish for behavioral pharmacological studies of drug screening, in special to psychedelic drug research.

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Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

It’s Tea Time: Interference of Ayahuasca Brew on Discriminative Learning in Zebrafish

Lobão-Soares

Eduardo-da-Silva

Amarilha

et al. 2018

Front. Behav. Neurosci.

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“…We used ZebTrack (Pinheiro-da-Silva et al, 2016) to extract from the video recordings six behavioural metrics that had previously been shown to describe well the stress response of zebrafish (Gerlai et al, 2008; Luca & Gerlai, 2012; Tran & Gerlai, 2013), namely : (1) mean swimming speed, (2) maximum swimming speed, (3) total distance travelled, (4) time spent freezing, (5) swimming depth (i.e. distance from the tank bottom) and (6) mean distance to the conspecific’s tank.…”

Section: Methodsmentioning

confidence: 99%

Fear contagion in zebrafish: a behaviour affected by familiarity

Silva

Leániz

Luchiari

2019

Preprint

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Emotional contagion has recently been described in fish but whether it is affected by familiarity is not known. We tested whether the sight of a distressed conspecific elicited fear in zebrafish, and whether this was modulated by familiarity. Groups of six zebrafish were housed together in the same tanks for 7 days to create familiar conditions. The behaviour of individual fish was then recorded in paired tanks within sight of either a familiar or an unfamiliar individual, before and after distilled water or an alarm substance was added to the demonstrator, but not to the observer. As expected, addition of distilled water did not elicit any behavioural change in either the demonstrator or the observer. However, addition of an alarm cue triggered anti-predatory behaviours in the demonstrator which caused the expression of anti-predatory behaviours in the observer, suggesting the existence of fear contagion. Furthermore, the extent of fear contagion was affected by familiarity, and observers were more active, swam closer to the bottom and further away from the demonstrator when they watched a distressed familiar neighbour than when they watched an unfamiliar fish. Our results have implications for fish welfare because they show that fish can become stressed by simply watching others become stressed. They also have implications for experimental design because fish housed in separate tanks cannot be assumed to be statistically independent if they can eavesdrop on their neighbours.

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Does early ethanol exposure increase seeking‐like behavior in zebrafish?

Pinheiro-da-Silva

Araujo-Silva

Luchiari

2021

Intl J of Devlp Neuroscience

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Fetal alcohol spectrum disorder (FASD) is the most common cause of birth defects. The severe variations are in fetal alcohol syndrome (FAS) but the most frequent cases are alcohol‐related neurodevelopmental disorder (ARND), which is of a difficult diagnosis. ARND characteristics include impaired social behavior, anxiety and depression prevalence, cognitive deficits, and an increased chance for drug addiction. Here, we aimed to test whether early alcohol exposure leads to later alcohol preference. We hypothesize that early alcohol exposure increases the reinforcing effects on later experiences, raising the chance of addiction in adult life. Lately, the zebrafish has been a valuable model on alcohol research, allowing embryonic exposure and the study of the ontogenetic effects. For this, embryos were exposed to three different alcohol treatments: 0.0%, 0.25% and 0.5%, for 2 hr, at 24‐hr post‐fertilization. Then we evaluated the effects of embryonic alcohol exposure on conditioned place preference in two developmental stage: fry (10 days post‐fertilization (dpf)) and young (90 dpf) zebrafish. Results show that control fish presented alcohol associative learning, which means, changes in place preference due to alcohol exposure, at both ontogenetic phases. However, zebrafish exposed to 0.25 and 0.5% alcohol during embryogenesis did not show conditioning response at any evaluated stage. These results suggest perception and cognitive deficits due to embryonic alcohol exposure that can alter alcohol responsiveness throughout a lifetime. Although low alcohol doses do not provoke malformation, it has been shown to induce several neurological and behavioral changes that are termed as Alcohol‐Related Neurodevelopmental Disorders. These results may contribute to future investigations on how embryonic exposure affects the neurocircuitry related to perception and associative learning processing.

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Sleep deprivation effects on object discrimination task in zebrafish (Danio rerio)

Cited by 54 publications

References 72 publications

It’s Tea Time: Interference of Ayahuasca Brew on Discriminative Learning in Zebrafish

It’s Tea Time: Interference of Ayahuasca Brew on Discriminative Learning in Zebrafish

Fear contagion in zebrafish: a behaviour affected by familiarity

Does early ethanol exposure increase seeking‐like behavior in zebrafish?

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