Sleep Deprivation and Activation of Morning Levels of Cellular and Genomic Markers of Inflammation

Abstract

Sleep loss induces a functional alteration of the monocyte proinflammatory cytokine response. A modest amount of sleep loss also alters molecular processes that drive cellular immune activation and induce inflammatory cytokines; mapping the dynamics of sleep loss on molecular signaling pathways has implications for understanding the role of sleep in altering immune cell physiologic characteristics. Interventions that target sleep might constitute new strategies to constrain inflammation with effects on inflammatory disease risk.

Depression, social support, and beta-adrenergic transcription control in human ovarian cancer

“…The present findings are consistent with other recent data linking biobehavioral factors to gene expression differences via signaling pathways such as the inflammation-related glucocorticoid receptor, NF-κB/Rel, and JAK/STAT transcription factors, as well as the CREB/ATF and Ets factors (Irwin, Wang et al 2006;Cole, Hawkley et al 2007;Miller, Chen et al 2008). This study is novel in identifying genome-wide transcriptional correlates of biobehavioral risk factors specifically within diseased tissue.…”

Psychoneuroimmunology of Early-Life Stress: The Hidden Wounds of Childhood Trauma?

“…Fifth, early-life stress has been associated with decreased total sleep and disruption in sleep architecture in rodents (Feng et al, 2007;Mrdalj et al, 2013;Tiba et al, 2004) and humans (Gregory and Sadeh, 2016;Kajeepeta et al, 2015). In turn, clinical experiments have shown that sleep deprivation is associated with an increase in the expression of pro-inflammatory cytokines in humans (Irwin et al, 2006), and epidemiological studies have found that reduced sleep is associated with elevated levels of inflammation biomarkers (Miller et al, 2009). Finally, early-life stress is associated with later abnormalities in brain functioning and behavior (Danese and McCrory, 2015).…”

A Functional Genomic Fingerprint of Chronic Stress in Humans: Blunted Glucocorticoid and Increased NF-κB Signaling

“…RNA was subsequently extracted using RNAlater/RNeasy (Qiagen, Valencia California). 5 mg of the resulting RNA was the assayed using Affymetrix U133A high-density oligonucleotide arrays (28) in the UCLA DNA Microarray Core as previously described (29,30). Robust Multiarray Averaging (31) was applied to quantify expression of the 22,283 assayed transcripts, and differentially expressed genes were identified as those showing ≥50% difference in mean expression levels between caregivers and controls (corresponding to a false discovery rate of 10%; 32).…”