Sleep, activity, temperature and arousal responses of mice deficient for muscarinic receptor M2 or M4

Turner, Jeremy G.; Hughes, Larry F.; Toth, Linda A

doi:10.1016/j.lfs.2009.11.019

Cited by 15 publications

(8 citation statements)

References 58 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, females, while not tested by Turner et al. (2010), in our study demonstrated increase in locomotor activity in their active phase only. Except for the work of Turner et al.…”

Section: Discussionsupporting

confidence: 45%

“…By contrast, M 4 KO male mice on pure 129SvEv background present increased locomotor activity only in the first 10 min (Koshimizu et al., 2012), and M 4 KO males fully backcrossed to the C57BL/6NTac genetic background do not differ in open‐field locomotor activity from wild‐type controls, in spite of altered dopaminergic responses (Fink‐Jensen et al., 2011). Moreover, M 4 KO males backcrossed to C57BL/6NTac genetic background do not differ with controls in the amount and diurnal pattern of sleep, locomotor activity, and temperature (Turner, Hughes, & Toth, 2010). To assess locomotor activity in undisturbed mice over a prolonged period (Turner et al., 2010), as we did, we employed a telemetric system.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, M 4 KO males backcrossed to C57BL/6NTac genetic background do not differ with controls in the amount and diurnal pattern of sleep, locomotor activity, and temperature (Turner, Hughes, & Toth, 2010). To assess locomotor activity in undisturbed mice over a prolonged period (Turner et al., 2010), as we did, we employed a telemetric system. Consistent with this report, we did not find marked differences in M 4 KO males.…”

Section: Discussionmentioning

confidence: 99%

“…Except for the work of Turner et al. (2010), all other reports tested locomotor activity only during the light period, which is the inactive phase of mice. It can be speculated about whether differences might be seen under the same experimental conditions (locomotor activity testing apparatus, gender, genetic background), but in the active phase of mice.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

The deletion of M₄ muscarinic receptors increases motor activity in females in the dark phase

et al. 2018

View full text Add to dashboard Cite

ObjectivesM4 muscarinic receptors (MR) presumably play a role in motor coordination. Previous studies have shown different results depending on genetic background and number of backcrosses. However, no attention has been given to biorhythms.Material and MethodsWe therefore analyzed biorhythms under a light/dark cycle obtained telemetrically in intact animals (activity, body temperature) in M4 KO mice growth on the C57Bl6 background using ChronosFit software. Studying pure effects of gene knockout in daily rhythms is especially important knowledge for pharmacological/behavioral studies in which drugs are usually tested in the morning.ResultsWe show that M4 KO mice motor activity does not differ substantially from wild‐type mice during light period while in the dark phase (mice active part of the day), the M4 KO mice reveal biorhythm changes in many parameters. Moreover, these differences are sex‐dependent and are evident in females only. Mesor, night–day difference, and night value were doubled or tripled when comparing female KO versus male KO. Our in vitro autoradiography demonstrates that M4 MR proportion represents 24% in the motor cortex (MOCx), 30% in the somatosensory cortex, 50% in the striatum, 69% in the thalamus, and 48% in the intergeniculate leaflet (IGL). The M4 MR densities were negligible in the subparaventricular zone, the posterior hypothalamic area, and in the suprachiasmatic nuclei.ConclusionsWe conclude that cholinergic signaling at M4 MR in brain structures such as striatum, MOCx, and probably with the important participation of IGL significantly control motor activity biorhythm. Animal activity differs in the light and dark phases, which should be taken into consideration when interpreting the results.

show abstract

“…However, females, while not tested by Turner et al. (2010), in our study demonstrated increase in locomotor activity in their active phase only. Except for the work of Turner et al.…”

Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The deletion of M₄ muscarinic receptors increases motor activity in females in the dark phase

et al. 2018

View full text Add to dashboard Cite

show abstract

“…However, our behavioral test was designed to measure PPI, and no different startle stimulus intensities were used. Anyway, in the present experiment, startle reflex can be also related to arousal or excitability in brainstem circuits, as previously described [47,48].…”

Section: Discussionsupporting

confidence: 70%

Finasteride administration potentiates the disruption of prepulse inhibition induced by forced swim stress

Pallarès

Llidó

Mòdol

et al. 2015

Behavioural Brain Research

View full text Add to dashboard Cite

Genetic Models of Sensorimotor Gating: Relevance to Neuropsychiatric Disorders

Powell

Weber²,

Geyer

2011

Current Topics in Behavioral Neurosciences

114

View full text Add to dashboard Cite

Sensorimotor gating, or the ability of a sensory event to suppress a motor response, can be measured operationally via prepulse inhibition (PPI) of the startle response. PPI is deficient in schizophrenia patients as well as other neuropsychiatric disorders, can be measured across species, and has been used widely as a translational tool in preclinical neuropharmacological and genetic research. First developed to assess drug effects in pharmacological and developmental models, PPI has become one of the standard behavioral measures in genetic models of schizophrenia and other neuropsychiatric disorders that exhibit PPI deficits. In this chapter we review the literature on genetic models of sensorimotor gating and discuss the utility of PPI as a tool in phenotyping mutant mouse models. We highlight the approaches to genetic mouse models of neuropsychiatric disease, discuss some of the important caveats to these approaches, and provide a comprehensive table covering the more recent genetic models that have evaluated PPI.

show abstract

Sleep, activity, temperature and arousal responses of mice deficient for muscarinic receptor M2 or M4

Cited by 15 publications

References 58 publications

The deletion of M₄ muscarinic receptors increases motor activity in females in the dark phase

The deletion of M₄ muscarinic receptors increases motor activity in females in the dark phase

Finasteride administration potentiates the disruption of prepulse inhibition induced by forced swim stress

Genetic Models of Sensorimotor Gating: Relevance to Neuropsychiatric Disorders

Contact Info

Product

Resources

About

Sleep, activity, temperature and arousal responses of mice deficient for muscarinic receptor M2 or M4

Cited by 15 publications

References 58 publications

The deletion of M4 muscarinic receptors increases motor activity in females in the dark phase

The deletion of M4 muscarinic receptors increases motor activity in females in the dark phase

Finasteride administration potentiates the disruption of prepulse inhibition induced by forced swim stress

Genetic Models of Sensorimotor Gating: Relevance to Neuropsychiatric Disorders

Contact Info

Product

Resources

About

The deletion of M₄ muscarinic receptors increases motor activity in females in the dark phase

The deletion of M₄ muscarinic receptors increases motor activity in females in the dark phase