SLE Single-Step Purification and HPLC Isolation Method for Sterols and Triterpenic Dialcohols Analysis from Olive Oil

Abstract

The unsaponifiable fraction of oils and fats constitutes a very small fraction but it is an essential part of the healthy properties of some specific oils. It is a complex fraction formed by a large number of minor compounds and it is a source of information to characterize and authenticate the oil sample. Specially, the composition of sterols of any oil or fat is a distinctive feature of itself and, therefore, it has become a useful tool for detecting contaminants and adulterants in oils. A new supported liquid extraction (SLE) technique for the analysis and characterization of the unsaponifiable fraction of fats and oils is proposed. The SLE system includes, as a stationary phase, a combination of adsorbent materials which allow a highly purified unsaponifiable matter ready to be isolated by high performance liquid chromatography (HPLC) and quantified by gas chromatography (GC). This method ensures the removal of fatty acids, avoiding possible interferences and making the analysis of sterols and triterpenic dialcohols easier. The procedure uses a small sample size (0.2 g), reduces the volume of solvents and reagents, and reduces the handling of samples subjected to analytical control. All this is achieved without losing either precision—a relative standard deviation of each compound lower than the reference value (≤16.4%)—or recovery, being for all compounds higher than 88.00%. Therefore, this new technique represents a significant economic and time saving in business control laboratories, a larger productivity and enhancement of working safety.

A Comparison of the Effects of Raw and Processed Amaranth Grain on Laying Hens’ Performance, Egg Physicochemical Properties, Blood Biochemistry and Egg Fatty Acids

“…In the “Specialized Food Laboratory Tekno Azma” in Tehran, Iran, which is approved by the Food and Drug Administration of Iran, the values of linoleic acid (LA) and squalene [ 25 ] were determined usinga GC device (Model: Younglin 6100, Anyang, Republic of Korea). Next, tocopherol content was determined by HPLC (Model: Younglin Acme 9000, Anyang, Republic of Korea), while a Jasco FP-4025 detector made in Vienna, Austria based on AOCS [ 26 ] was used to measure phytosterols [ 27 , 28 ] ( Table 2 ).…”

Food Authentication: Techniques, Trends and Emerging Approaches (Second Issue)

“…As an alternative approach, other authors reported the use of chromatography-based techniques for authenticity and traceability purposes. León-Camacho and Pérez-Camino developed a new supported liquid extraction (SLE) method that, in combination with high-performance liquid chromatography (HPLC) and GC-FID, simplifies the isolation and quantification of the unsaponifiable fraction from fats and oils [8]. This procedure is easier, less time-consuming, and reduces the volume of solvents and reagents compared to traditional liquid-liquid extraction.…”