Slab waveguide-based particle plasmon resonance optofluidic biosensor for rapid and label-free detection

Abstract

An effective bio-sensing platform that would meet the criteria of rapid, simple, and sensitive detection is crucial to translate bench research to clinical applications. However, simultaneously rapid and sensitive biosensing...

Ultrasensitive and Rapid Detection of Procalcitonin via Waveguide-Enhanced Nanogold-Linked Immunosorbent Assay for Early Sepsis Diagnosis

“…Here, we propose and develop waveguide-enhanced nanogold-linked immunosorbent assay (WENLISA) biosensors for sensitive and rapid PCT detection as a key enabler for early sepsis diagnosis. The WENLISA biosensor comprises a suspended glass planar WG on a glass substrate integrated with a microfluidic channel fabricated using simple, cost-effective, and vacuumless processes . In addition, to enhance the LOD to meet the clinical requirements for PCT detection, we introduced a new sensing strategy using gold nanoparticles (AuNPs) with a unique sandwich architecture.…”

“…After a stable baseline was observed in the blank sample, a PCT standard solution was injected into the sensing region, and the transmitted light intensity suddenly dropped. This intensity drop is attributed to the RI change and the extinction of light by the AuNPs mainly through absorption as they have a large extinction coefficient of k = 2.2 at λ = 521.5 nm . Subsequently, the transmitted light intensity decreased exponentially.…”