Slab Optical Waveguide with Octadecylsilane Coated Surface for Absorption Detector of Flow Analysis

Abstract

A K+ doped glass slab optical waveguide (SOWG) was coated with octadecylsilane (ODS) to separate and concentrate analytes onto the SOWG with hydrophobic interaction between analytes and ODS. As an absorption detector of flow analysis, the ODS–SOWG detector was almost insensitive to a blue dye (bromothymol blue) which is not adsorbed to the surface of the SOWG. On the other hand, it was highly sensitive to another blue dye of coomassie brilliant blue G-25O (CBB) which is firmly adsorbed onto the SOWG surface.

Adsorption Behavior of Cytochrome c, Myoglobin and Hemoglobin in a Quartz Surface Probed Using Slab Optical Waveguide (SOWG) Spectroscopy

“…Since the interacting photons come from the waveguiding surface, one advantage of ITR-based spectroscopy is its ability to interact mostly with the adsorbed molecules and much less with those in the bulk solution. Some of the research groups that are actively involved in studying protein adsorption on surfaces based on ITR are Jeremy Ramsden's group in Basel, Switzerland, 28-37 Scott Saavedra's group in Arizona, [18][19][20][21][22][23][24][25][26][27] Paul R. Van Tassel's group in Wayne, USA 38,40 and some research groups in Japan [4][5][6][7][8][9][10][11][12][13][14][15][16][17]39 and elsewhere. 41,42 In this work we investigated, under various solution conditions, the adsorption behavior of hemoglobin, myoglobin and cytochrome c in a bare quartz surface.…”

Characteristics of Sensor Response of a Difference Interferometric Slab Optical Waveguide Refractive Index Sensor with a Prism Coupling Method

“…2a) was fabricated by an ion-exchange process on commercial sodalime slide glasses (S-1214 from Matsunami Glass Inc., Japan) in molten potassium nitrate at 673 K for 30 min. 8,9 The second one is a glass SOWG having a tapered velocity coupler (SP-SOWG, Fig. 2b), which was originally proposed by Itoh and Madou to enhance the sensitivity of the SOWG measurements.…”

Adsorption Behavior of Curcumin onto Cetyltrimethylammonium Chloride Reverse Micelles Immobilized on a Glass Surface As Studied by Polarized Visible Attenuated Total Reflection Spectrometry with a Glass Slab Optical Waveguide

“…11,12 Moreover, it is applicable to molecules that are not fluorophores, and information on the orientation of adsorbed molecules can be obtained with polarization (linear dichroism) measurements. [13][14][15] We have been using glass SOWGs for chemical sensing of traces of various compounds, [16][17][18] electrospectrochemistry, 19,20 and the characterization of modified and unmodified glass surfaces using dye molecules with polarization measurements. 14,21,22 In this study, polarized visible ATR spectrometry with a glass SOWG was applied to study the adsorption behavior of a hydrophobic dye, curcumin, onto the CTAC reverse micelle immobilized on a glass surface to characterize the immobilized CTAC reverse micelle.…”