SKP2, positively regulated by circ_ODC1/miR‐422a axis, promotes the proliferation of retinoblastoma

Du, Shanshan; Wang, Shuai; Zhang, Fengyan; Lv, Yong

doi:10.1002/jcb.29177

Cited by 19 publications

(13 citation statements)

References 33 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Functionally, circRNA participates in the formation of tumors, regulating malignant biological behaviors of cancer cells such as proliferation, apoptosis and metastasis. It is reported that circ_0001649 suppresses RB cell proliferation and apoptosis by inhibiting AKT/mTOR signaling pathway, and its low expression level indicates poor prognosis in patients [23]; circ_ODC1 positively regulates SKP2 and promotes RB cell proliferation [24]. In this study, we found for the first time that the expression level of circ_0000527 in RB tissues and cells was significantly increased, and its high expression was significantly correlated with the lower the degree of differentiation and increased cTNM staging.…”

Section: Discussionmentioning

confidence: 52%

Circ_0000527 promotes the progression of retinoblastoma by regulating miR-646/LRP6 axis

Zhang¹,

Wu²,

Li³

et al. 2020

Cancer Cell Int

View full text Add to dashboard Cite

Background: Researches validate that circular RNAs (circRNAs) are dysregulated in a variety of malignancies and play an important role in regulating the malignant phenotype of tumor cells. Nevertheless, the role of circ_0000527 in retinoblastoma (RB) and its regulatory mechanisms remain largely unknown. Methods: Real-time PCR (RT-PCR) was used to detect circ_0000527 and miR-646 expression in RB tissues and cells. The LRP6 expression in RB cells was detected by Western blot. The relationship between circ_0000527 expression and the clinicopathological parameters of RB patients was analyzed. Cell proliferation, apoptosis and metastasis were monitored by cell counting kit-8 (CCK-8), flow cytometry, and Transwell assay. The dual-luciferase reporter gene assay and RIP assay were employed to verify the targeting relationship between circ_0000527 and miR-646, miR-646 and LRP6. Results: Circ_0000527 was highly expressed in both RB and RB cell lines, whose high expression level and degree of differentiation were significantly correlated with the increase in cTNM staging level. Overexpression of circ_0000527 contributed to RB cell proliferation, migration, invasion and suppressed cell apoptosis, while knocking down circ_0000527 inhibited the above malignant biological behavior. The underlying mechanism suggested that functioning as a endogenous competitive RNA, circ_0000527 directly targeted miR-646 and positively regulated LRP6 expression. Conclusion: Circ_0000527 enhances the proliferation and metastasis of RB cells by modulating the miR-646/LRP6 axis.

show abstract

Section: Discussionmentioning

confidence: 52%

Circ_0000527 promotes the progression of retinoblastoma by regulating miR-646/LRP6 axis

Zhang¹,

Wu²,

Li³

et al. 2020

Cancer Cell Int

View full text Add to dashboard Cite

show abstract

“…By means of qRT-PCR, we verified the negative correlation between the three miRNAs and three ubiquitination-related genes (CBL, SMURF2, and USP4), which are key components of the ubiquitin–proteasome system and have been shown to engage in the ubiquitination and degradation of some pivotal genes implicated in HTS [ 23 , 24 ]. Consistent with these findings, miR-422a was reported to directly target SKP2 [ 25 ], which is an E3 ubiquitin ligase and was confirmed to promote fibroblast proliferation in pulmonary fibrosis through via ubiquitin–proteasome pathway [ 26 ]. In addition, miR-2116-3p was shown to interact with and inhibit PIWIL1 [ 27 ], and silencing of PIWIL1 was reported to upregulate FBXW7 [ 28 ], which is a component of E3 ubiquitin-protein ligase complex and was observed to induce expression of fibrotic genes including fibronectin during pulmonary fibrosis [ 29 ].…”

Section: Discussionmentioning

confidence: 70%

Identification and functional analysis of a three-miRNA ceRNA network in hypertrophic scars

et al. 2021

View full text Add to dashboard Cite

Background Hypertrophic scar (HTS) is a fibrotic disorder of skins and may have repercussions on the appearance as well as functions of patients. Recent studies related have shown that competitive endogenous RNA (ceRNA) networks centering around miRNAs may play an influential role in HTS formation. This study aimed to construct and validate a three-miRNA (miR-422a, miR-2116-3p, and miR-3187-3p) ceRNA network, and explore its potential functions. Methods Quantitative real‑time PCR (qRT‑PCR) was used to compare expression levels of miRNAs, lncRNAs, and genes between HTS and normal skin. Target lncRNAs and genes of each miRNA were predicted using starBase as well as TargetScan database to construct a distinct ceRNA network; overlapping target lncRNAs and genes of the three miRNAs were utilized to develop a three-miRNA ceRNA network. For every network, protein–protein interaction (PPI) network analysis was performed to identify its hub genes. For each network and its hub genes, Gene Oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted to explore their possible functions. Results MiR-422a, miR-2116-3p, and miR-3187-3p were all downregulated in HTS tissues and fibroblasts. MiR-422a-based ceRNA network consisted of 101 lncRNAs with 133 genes; miR-2116-3p-centered ceRNA network comprised 85 lncRNAs and 978 genes; miR-3187-3p-derived ceRNA network encompassed 84 lncRNAs as well as 1128 genes. The three-miRNA ceRNA network included 2 lncRNAs with 9 genes, where MAPK1, FOSL2, ABI2, KPNA6, CBL, lncRNA-KCNQ1OT1, and lncRNA-EBLN3P were upregulated. According to GO and KEGG analysis, these networks were consistently related to ubiquitination. Three ubiquitination-related genes (CBL, SMURF2, and USP4) were upregulated and negatively correlated with the expression levels of the three miRNAs in HTS tissues. Conclusions This study identified a three-miRNA ceRNA network, which might take part in HTS formation and correlate with ubiquitination.

show abstract

“…For example, dysregulation of Skp2 expression plays a carcinogenic role in hematological malignancies [ 23 ]. The overexpression of Skp2 in retinoblastoma can restore suppressed retinoblastoma growth [ 24 ]. Therefore, many experts and scholars are using Skp2 as a target for cancer treatment to explore more cancer treatment methods.…”

Section: Discussionmentioning

confidence: 99%

miR-590-5p Targets Skp2 to Inhibit the Growth and Invasion of Malignant Melanoma Cells

Tong

Jin

2022

Disease Markers

View full text Add to dashboard Cite

Skp2 participates in the regulation of cell growth cycle and promotes the growth of tumor cells. It was speculated that miR-590-5p could regulate the expression of Skp2 and have therapeutic effects on malignant melanin. In this study, the expression of Skp2 was detected by qRT-PCR and Western blot (WB), and the targeted binding between miR-590-5p and Skp2 was verified by dual luciferase reporting assay. Subsequently, cell proliferation activity was detected by CCK8, cell invasion was detected by Transwell, and cell apoptosis was detected by mitochondrial membrane potential assay. The results indicate that Skp2 is highly expressed in melanoma cells and inhibits the proliferation and invasion of melanoma cells. However, miR-590-5p can bind to Skp2 in a targeted manner. miR-590-5p is underexpressed in melanoma cells, and its overexpression can inhibit Skp2 expression and proliferation and invasion of melanoma cells. Our results showed that miR-590-5p could inhibit melanoma cell development by targeting Skp2. This study provides more therapeutic targets for the treatment of melanoma.

show abstract

SKP2, positively regulated by circ_ODC1/miR‐422a axis, promotes the proliferation of retinoblastoma

Cited by 19 publications

References 33 publications

Circ_0000527 promotes the progression of retinoblastoma by regulating miR-646/LRP6 axis

Circ_0000527 promotes the progression of retinoblastoma by regulating miR-646/LRP6 axis

Identification and functional analysis of a three-miRNA ceRNA network in hypertrophic scars

miR-590-5p Targets Skp2 to Inhibit the Growth and Invasion of Malignant Melanoma Cells

Contact Info

Product

Resources

About