Skipping of exon 10 in<i> Axl</i> pre-mRNA regulated by PTBP1 mediates invasion and metastasis process of liver cancer cells

Shen, Lianghua; Lei, Sijia; Zhang, Bin; Li, Shuaiguang; Huang, Luyuan; Czachor, Alexander; Breitzig, Mason; Gao, Yuman; Huang, Meiyan; Mo, Xuemei; Zheng, Qi; Sun, Han; Wang, Feng

doi:10.7150/thno.42010

Cited by 43 publications

(28 citation statements)

References 54 publications

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“…Wound healing assay was performed as previously described ( Shen et al, 2020 ). CT26 cells were seeded into 6-well plates and cultured to confluence.…”

Section: Methodsmentioning

confidence: 99%

Albuca Bracteate Polysaccharides Synergistically Enhance the Anti-Tumor Efficacy of 5-Fluorouracil Against Colorectal Cancer by Modulating β-Catenin Signaling and Intestinal Flora

Xue

Tan

et al. 2021

Front. Pharmacol.

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The first-line treatment for colorectal cancer (CRC) is 5-fluorouracil (5-FU). However, the efficacy of this treatment is sometimes limited owing to chemoresistance as well as treatment-associated intestinal mucositis and other adverse events. Growing evidence suggests that certain phytochemicals have therapeutic and cancer-preventing properties. Further, the synergistic interactions between many such plant-derived products and chemotherapeutic drugs have been linked to improved therapeutic efficacy. Polysaccharides extracted from Albuca bracteata (Thunb.) J.C.Manning and Goldblatt (ABP) have been reported to exhibit anti-oxidant, anti-inflammatory, and anti-tumor properties. In this study, murine CRC cells (CT26) and a murine model of CRC were used to examine the anti-tumor properties of ABP and explore the mechanism underlying the synergistic interactions between ABP and 5-FU. Our results revealed that ABP could inhibit tumor cell proliferation, invasion, and migratory activity in vitro and inhibited tumor progression in vivo by suppressing β-catenin signaling. Additionally, treatment with a combination of ABP and 5-FU resulted in better outcomes than treatment with either agent alone. Moreover, this combination therapy resulted in the specific enrichment of Ruminococcus, Anaerostipes, and Oscillospira in the intestinal microbiota and increased fecal short-chain fatty acid (SCFA) levels (acetic acid, propionic acid, and butyric acid). The improvement in the intestinal microbiota and the increase in beneficial SCFAs contributed to enhanced therapeutic outcomes and reduced the adverse effects of 5-FU. Together, these data suggest that ABP exhibits anti-neoplastic activity and can effectively enhance the efficacy of 5-FU in CRC treatment. Therefore, further research on the application of ABP in the development of novel anti-tumor drugs and adjuvant compounds is warranted and could improve the outcomes of CRC patients.

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“…Wound healing assay was performed as previously described ( Shen et al, 2020 ). CT26 cells were seeded into 6-well plates and cultured to confluence.…”

Section: Methodsmentioning

confidence: 99%

Albuca Bracteate Polysaccharides Synergistically Enhance the Anti-Tumor Efficacy of 5-Fluorouracil Against Colorectal Cancer by Modulating β-Catenin Signaling and Intestinal Flora

Xue

Tan

et al. 2021

Front. Pharmacol.

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“…AS often occurs in a cell or tissue type-specific manner and is instrumental for cell specification, tissue development and organ function maintenance 12 . Dysregulation of AS can lead to severe malfunction and disease 13 , 14 . A considerable number of AS events have been identified during myogenesis in the past few years 15 .…”

Section: Introductionmentioning

confidence: 99%

Rbm24 modulates adult skeletal muscle regeneration via regulation of alternative splicing

Zhang¹,

Han²,

Liu³

et al. 2020

Theranostics

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Rationale: The adult skeletal muscle can self-repair efficiently following mechanical or pathological damage due to its remarkable regenerative capacity. However, regulatory mechanisms underlying muscle regeneration are complicated and have not been fully elucidated. Alternative splicing (AS) is a major mechanism responsible for post-transcriptional regulation. Many aberrant AS events have been identified in patients with muscular dystrophy which is accompanied by abnormal muscle regeneration. However, little is known about the correlation between AS and muscle regeneration. It has been reported that RNA binding motif protein 24 (Rbm24), a tissue-specific splicing factor, is involved in embryo myogenesis while the role of Rbm24 in adult myogenesis (also called muscle regeneration) is poorly understood. Methods: To investigate the role of Rbm24 in adult skeletal muscle, we generated Rbm24 conditional knockout mice and satellite cell-specific knockout mice. Furthermore, a cardiotoxin (CTX)-induced injury model was utilized to assess the effects of Rbm24 on skeletal muscle regeneration. Genome-wide RNA-Seq was performed to identify the changes in AS following loss of Rbm24. Results: Rbm24 knockout mice displayed abnormal regeneration 4 months after tamoxifen treatment. Using RNA-Seq, we found that Rbm24 regulated a complex network of AS events involved in multiple biological processes, including myogenesis, muscle regeneration and muscle hypertrophy. Moreover, using a CTX-induced injury model, we showed that loss of Rbm24 in skeletal muscle resulted in myogenic fusion and differentiation defects and significantly delayed muscle regeneration. Furthermore, satellite cell-specific Rbm24 knockout mice recapitulated the defects in regeneration seen in the global Rbm24 knockout mice. Importantly, we demonstrated that Rbm24 regulated AS of Mef2d, Naca, Rock2 and Lrrfip1 which are essential for myogenic differentiation and muscle regeneration. Conclusions: The present study demonstrated that Rbm24 regulates dynamic changes in AS and is essential for adult skeletal muscle regeneration.

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“…The exons 2–5 of AXL form two Ig-like domains, which are used to bind the sex hormone-binding globulin (SHBG) region of Gas6. The affinity between Gas6 and AXL is 3–10 times higher than Mer and Tyro3 ( Shen et al., 2020 ). In the AXL-Gas6 complex, both of the Ig-like domains of AXL are simultaneously connected to the first laminin G-like domain in Gas6 ( Sasaki et al., 2006 ).…”

Section: Structure Of Axlmentioning

confidence: 95%

“…AXL, also known as UFO, was discovered from two patients with chronic myeloid leukemia in 1988 ( O’Bryan et al., 1991 ). The human AXL gene which encodes 20 exons is ubiquitously expressed in various human organs, including brain, heart, liver, kidney, testis, uterus, and skeletal muscle ( Shen et al., 2020 ) ( Figure 2 ).…”

Section: Biology Of Axlmentioning

confidence: 99%

AXL, an Important Host Factor for DENV and ZIKV Replication

Xie

Zhang

Liang

et al. 2021

Front. Cell. Infect. Microbiol.

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Flaviviruses, as critically important pathogens, are still major public health problems all over the world. For instance, the evolution of ZIKV led to large-scale outbreaks in the Yap island in 2007. DENV was considered by the World Health Organization (WHO) as one of the 10 threats to global health in 2019. Enveloped viruses hijack a variety of host factors to complete its replication cycle. Phosphatidylserine (PS) receptor, AXL, is considered to be a candidate receptor for flavivirus invasion. In this review, we discuss the molecular structure of ZIKV and DENV, and how they interact with AXL to successfully invade host cells. A more comprehensive understanding of the molecular mechanisms of flavivirus-AXL interaction will provide crucial insights into the virus infection process and the development of anti-flavivirus therapeutics.

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Skipping of exon 10 in Axl pre-mRNA regulated by PTBP1 mediates invasion and metastasis process of liver cancer cells

Cited by 43 publications

References 54 publications

Albuca Bracteate Polysaccharides Synergistically Enhance the Anti-Tumor Efficacy of 5-Fluorouracil Against Colorectal Cancer by Modulating β-Catenin Signaling and Intestinal Flora

Albuca Bracteate Polysaccharides Synergistically Enhance the Anti-Tumor Efficacy of 5-Fluorouracil Against Colorectal Cancer by Modulating β-Catenin Signaling and Intestinal Flora

Rbm24 modulates adult skeletal muscle regeneration via regulation of alternative splicing

AXL, an Important Host Factor for DENV and ZIKV Replication

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