SKIP and BIR-1/Survivin have potential to integrate proteome status with gene expression

Kostrouch, David; Yilma, Petr; Chughtai, Ahmed Ali; Novotný, Jan; Novák, Petr; Kostrouchová, Veronika; Kostrouch, Zdeněk

doi:10.1016/j.jprot.2014.07.023

Cited by 3 publications

(5 citation statements)

References 49 publications

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“…Numerous transcription factors are regulated by their spatial restriction, binding or incorporation into cytoplasmic structures and organelles. Many proteins that have primarily cytoplasmic structural functions were shown to possess transcription regulating activity (e.g., proteins interacting with steroid receptors (George, Schiltz & Hager, 2009), FOX transcription factors (Gan et al, 2005; Wang et al, 2015), and BIR-1/Survivin (Kostrouch et al, 2014)). In-between interactions of MDT-28 that we identified, the interaction with MDT-30 may suggest an additional link towards connection of structural signals with the regulation of gene expression.…”

Section: Discussionmentioning

confidence: 99%

“…Binding studies were done as described (Kostrouch et al, 2014) with modifications. The coding region of mdt-6 was amplified using primers 8292 and 8293 from cDNA prepared from mixed stages C. elegans cultures and cloned into pTNT vector (Promega, Madison, WI, USA, amplified with primers 8277 and 8278) using the Quick Ligation Kit (New England Biolabs, Ipswich, MA, USA) and expressed in the rabbit reticulocyte TNT-system (Promega, Madison, WI, USA).…”

Section: Methodsmentioning

confidence: 99%

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The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator ofC. elegansdevelopment

Kostrouch

Chughtai

Kaššák

et al. 2017

PeerJ

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The evolutionarily conserved Mediator complex is a critical player in regulating transcription. Comprised of approximately two dozen proteins, the Mediator integrates diverse regulatory signals through direct protein-protein interactions that, in turn, modulate the influence of Mediator on RNA Polymerase II activity. One Mediator subunit, MED28, is known to interact with cytoplasmic structural proteins, providing a potential direct link between cytoplasmic dynamics and the control of gene transcription. Although identified in many animals and plants, MED28 is not present in yeast; no bona fide MED28 has been described previously in Caenorhabditis elegans. Here, we identify bioinformatically F28F8.5, an uncharacterized predicted protein, as the nematode homologue of MED28. As in other Metazoa, F28F8.5 has dual nuclear and cytoplasmic localization and plays critical roles in the regulation of development. F28F8.5 is a vital gene and its null mutants have severely malformed gonads and do not reproduce. F28F8.5 interacts on the protein level with the Mediator subunits MDT-6 and MDT-30. Our results indicate that F28F8.5 is an orthologue of MED28 and suggest that the potential to link cytoplasmic and nuclear events is conserved between MED28 vertebrate and nematode orthologues.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator ofC. elegansdevelopment

Kostrouch

Chughtai

Kaššák

et al. 2017

PeerJ

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many proteins that have primarily cytoplasmic structural functions were shown to possess transcription regulating activity (e.g. proteins interacting with steroid receptors (George et al 2009), FOX transcription factors (Gan et al 2005;Wang et al 2015), and BIR-1/Survivin (Kostrouch et al 2014)). In-between interactions of MDT-28 that we identified, the interaction with MDT-30 may suggest an additional link towards connection of structural signals with the regulation of gene expression.…”

Section: Discussionmentioning

confidence: 99%

“…Binding studies were done as described (Kostrouch et al 2014) Manuscript to be reviewed agarose (Sigma-Aldrich, St. Louis, MO, USA) adsorbed with equal amounts of GST or GST-F28F8.5. Radioactively labeled proteins were detected using TRI-CARB 1600TR, Liquid Scintillation Analyzer (Packard, Meriden, CT, USA).…”

Section: Binding Studiesmentioning

confidence: 99%

Peer Review #2 of "The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development (v0.2)"

2017

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The evolutionarily conserved Mediator complex is a critical player in regulating transcription. Comprised of approximately two dozen proteins, the Mediator integrates diverse regulatory signals through direct protein-protein interactions that, in turn, modulate the influence of Mediator on RNA Polymerase II activity. One Mediator subunit, MED28, is known to interact with cytoplasmic structural proteins, providing a potential direct link between cytoplasmic dynamics and the control of gene transcription. Although identified in many animals and plants, MED28 is not present in yeast; no bona fide MED28 has been described previously in C. elegans. Here, we identify bioinformatically F28F8.5, an uncharacterized predicted protein, as the nematode homologue of MED28. As in other Metazoa, F28F8.5 has dual nuclear and cytoplasmic localization and plays critical roles in the regulation of development. F28F8.5 is a vital gene and its null mutants have severely malformed gonads and do not reproduce. F28F8.5 interacts on the protein level with the Mediator subunits MDT-6 and MDT-30. Our results indicate that F28F8.5 is an orthologue of MED28 and suggest that the potential to link cytoplasmic and nuclear events is conserved between MED28 vertebrate and nematode orthologues.

show abstract

“…Many proteins that have primarily cytoplasmic structural functions were shown to possess transcription regulating activity (e.g. proteins interacting with steroid receptors (Biddie & Hager 2009), FOX transcription factors (Gan et al 2005;Wang et al 2015), and BIR-1/Survivin (Kostrouch et al 2014)). The direct link between effector proteins and the regulation of transcription can be traced to Eubacteria and Archaea.…”

Section: Discussionmentioning

confidence: 99%

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The evolutionarily conserved Mediator complex is a critical player in regulating transcription. Comprised of approximately two dozen proteins, Mediator integrates diverse regulatory signals through direct protein-protein interactions that, in turn, modulate the influence of Mediator on RNA Polymerase II activity. One Mediator subunit, MED28, is known to interact with cytoplasmic structural proteins, providing a potential direct link between cytoplasmic dynamics and the control of gene transcription. Although identified in many animals and plants, MED28 is not present in yeast; no bona fide MED28 has been described previously in C. elegans. Here, we identify bioinformatically F28F8.5, an uncharacterized predicted protein, as the nematode homologue of MED28. As in other metazoa, F28F8.5 has dual nuclear and cytoplasmic localization and plays critical roles in the regulation of development. F28F8.5 is a vital gene and its null mutants have severely malformed gonads and do not reproduce. Our results indicate that F28F8.5 is a homologue of MED28 and suggest that the potential to link cytoplasmic and nuclear events is conserved between MED28 vertebrate and nematode homologues.

show abstract

SKIP and BIR-1/Survivin have potential to integrate proteome status with gene expression

Cited by 3 publications

References 49 publications

The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator ofC. elegansdevelopment

The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator ofC. elegansdevelopment

Peer Review #2 of "The nematode homologue of Mediator complex subunit 28, F28F8.5, is a critical regulator of C. elegans development (v0.2)"

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