Skin Wrinkles Improvement Effect for Jeju Aloe arborescens Oil Isolated from Supercritical Fluid Extraction

Abstract

Purpose: In this study, the cytotoxicity and antioxidant effects of Aloe arborescens oil isolated from supercritical fluid extraction were confirmed, and an aloe oil cream was manufactured to evaluate the skin wrinkle improvement effect. Thus, we provided scientific evidence for functional cosmetic ingredients.Methods: Aloe oil was extracted from Jeju <i>Aloe arborescens</i> using a supercritical fluid extraction method. Aloe oil chromaticity and fatty acid analysis were determined. MTT assay and DPPH radical scavenging activity were used to assess the cell viability and antioxidant effect of NIH-3T3, HaCaT, and B16F10 cells. Additionally, we evaluated the effect of an aloe-containing cream on skin wrinkle improvement on 22 adult women 40–60 years of age.Results: Aloe oil extracted from Aloe arborescens using supercritical fluid extraction contained 0.02 g of fatty acid per 100 g, which included behenic, palmitic, α-linolenic, oleic, linoleic, and elaidic acids. The L value was 12.46±2.15, the a value was 3.73±0.26, the b value was 10.35±0.51, and the ΔE was 81.4 as a result of chromaticity. The cell viability of NIH-3T3, HaCaT, and B16F10 cells showed low cytotoxicity up to a concentration of 100 μg/mL. Aloe oil outperformed aloe supercritical extract in terms of DPPH radical scavenging activity. According to the results of a skin wrinkle improvement efficacy test, a cream containing aloe oil has a significantly high skin wrinkle improvement effect.Conclusion: We discovered that aloe oil isolated from supercritical fluid extraction has a strong antioxidant effect and significantly improves skin wrinkles.

Efficacy in vitro Antioxidation and in vivo Skin Barrier Recovery of Composition Containing Mineral-cation-phyto DNA Extracted from Aloe vera Adventitious Root

“…30 min 반응시켜 ELISA (Sigma-Aldrich)를 이용하여 517 nm에서 흡광도를 측정하였다. 계산식은 다음과 같이 계산하였다(Kim et al, 2022).DPPH radical scavenging activity (%)=100-[(OD of sample/OD of blank)×100] 3) 청색광 조사에 의한 세포 독성 완화 효과 측정 본 Method 5-B에서 수득된 알로에 베라 부정근의 조직배양 피 토 DNA 복합물 및 미네랄 복합물(Sample 2-1~4) 중, Sample 2-4 (1:10)의 복합물을 처리한 후 세포의 생장을 관찰하였다. 서울대학교 의과대학 피부과 실험실에서 공급받은 HaCaT 세포를 6 well plate 에 1×10 6 cells/well의 농도로 분주하여 배양한 후, 10% FBS가 첨가 된 배지로 교체하여 18 h 동안 결핍(starvation)시키고, 24 h 동안 배 양하였다.…”