Skin Wound Healing: Some Biochemical Parameters in Guinea-pig

Abstract

Hairs were removed from the dorsal skin of guinea-pigs and 5-6 wounds (7 x 7 mm) were surgically induced by totally removing the epidermal and part of the dermal surface. They were then allowed to heal. The newly formed wound tissues were dissected at different times during the process and analysed by biochemical and histological methods. Hydroxyproline, proteins, DNA and semicarbazide-sensitive amine oxidase (SSAO) were measured, as were [14C]leucine and [3H]thymidine incorporation in some samples. The peroxidase-like activity of plasma albumin and the histology of wounds stained with haematoxylin-eosin were also studied. It was shown that SSAO enzymes, which are present in normal guinea-pig skin and have a high affinity for benzylamine are localized in fibroblasts. During skin healing in the newly formed tissue there was an increase in protein content which reached a maximum after 4-6 days; DNA content also increased. The rate of incorporation of [3H]thymidine and [14C]leucine paralleled DNA and protein content, respectively. The content of hydroxyproline had greatly decreased with respect to that in normal skin after 2-10 days. SSAO activity increased much less than DNA after 4 days whereas after 10-11 days it increased more than DNA, thus indicating that at this time it was probably produced by fibroblasts. No significant increase in the peroxidase-like activity of albumin was observed 4, 8 or 11 days after surgery. Treatment of the animals with methylprednisolone acetate (20 mg kg-1, i.m.) two days before surgery decreased the rate of skin healing but did not alter the level of albumin peroxidase activity of the plasma.(ABSTRACT TRUNCATED AT 250 WORDS)

An ethnobotanical study of traditional anti-inflammatory plants used by the Lohit community of Arunachal Pradesh, India

“…The precise mechanism of wound healing is not clearly understood. The process of wound repair appears to be very complex involving various parameters such as inflammations, cell proliferations, collagen lattice formation, microbial infections, oxygen free-radicals, epithelization, anti-oxidant defense and biochemical change in hydroxyproline (Bodeker and Hughes, 1998;Martin, 1996;Clark, 1996;Buffoni et al, 1993). Hence, the existing pharmacological validation of plant active compounds should realize several factor(s) or parameter(s) involved in wound repair.…”

Ethnopharmacological approaches to wound healing—Exploring medicinal plants of India

“…It involves platelet aggregation and blood clotting, formation of fibrin, an inflammatory response to injury, alteration in the ground substances, angiogenesis and re-epithelialization. Healing is not complete until the disrupted surfaces are firmly knit by collagen (Buffoni et al, 1993). The basic principle of optimal wound healing is to minimize tissue damage and provide adequate tissue perfusion and oxygenation, proper nutrition and moist wound healing environment to restore the anatomical continuity and function of the affected part (Pierce and Mustoe, 1995).…”

Effects of Pulsed 810 nm Al‐Ga‐As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight

“…Non‐irradiated control rats were left to heal naturally. The previous studies have reported that markers related with wound repair process were found to be prominently expressed in the first week of post‐wounding . Hence, in the present study wound tissues were collected for the evaluation of biochemical and molecular markers after 7 days of post‐wounding.…”