Skin tissue engineering using 3D bioprinting: An evolving research field

Tarassoli, Sam P.; Jessop, Zita M.; Al‐Sabah, Ayesha; Gao, Neng; Whitaker, Sairan; Doak, Shareen H.; Whitaker, Iain S.

doi:10.1016/j.bjps.2017.12.006

Cited by 156 publications

(134 citation statements)

References 62 publications

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“…Appropriate cell selection is an important premise before 3D bioprinting for skin regeneration (Table ). Currently, the most common cell types used in skin substitutes include keratinocytes (KCs) and fibroblasts (FBs), which form the outer epidermis and the dermal skin substitutes, respectively . Although the KCs, which have a strong ability of anti‐aging, can be cultured easily in the laboratory, using single cells cannot print full‐featured skin substitutes.…”

Section: Cell Selectionmentioning

confidence: 99%

“…In addition, FBs in different sites have different functional characteristics, which should be considered before tissue engineering selection as this may affect the applicability of their dermal substitutes. Lee et al first combined KCs with FBs to bioprint skin substitutes in vitro . Nieves Cubo et al used primary human FBs and human KCs as well as human plasma to print a human bi‐layered skin.…”

Section: Cell Selectionmentioning

confidence: 99%

“…The laser‐assisted bioprinter is based on laser‐induced forward transfer . Michael et al formulated a mixture comprised of FBs and KCs with hydrocortisone.…”

Section: D Bioprintermentioning

confidence: 99%

“…Bioink is critical to ensure the viability and responsiveness of cells in 3D‐printed architectures. It must be biocompatible, biodegradable, printable, bioinert, strong, and durable as well as ductile . According to their source of production, the bioink can be roughly divided into two types, which are called natural biopolymer and synthetic biopolymer .…”

Section: Bioinkmentioning

confidence: 99%

“…According to their source of production, the bioink can be roughly divided into two types, which are called natural biopolymer and synthetic biopolymer . Natural biopolymers containing proteins and polysaccharides are naturally produced; the proteins include collagen, gelatin, albumin, thrombin, fibrinogen and so on, and the polysaccharides include chitosan, chitin, cellulose, and so forth . Synthetic biopolymers are artificial polymers that include polyglycolic acid (PGA), polylactic acid (PLA), poly(lactic‐co‐glycolic acid) (PLGA), poly(ε‐caprolactone) (PCL), and so on .…”

Section: Bioinkmentioning

confidence: 99%

See 4 more Smart Citations

Beyond 2D: 3D bioprinting for skin regeneration

Wang

Yao

et al. 2018

International Wound Journal

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Essential cellular functions that are present in tissues are missed by two‐dimensional (2D) cell monolayer culture. It certainly limits their potential to predict the cellular responses of real organisms. Engineering approaches offer solutions to overcome current limitations. For example, establishing a three‐dimensional (3D)‐based matrix is motivated by the need to mimic the functions of living tissues, which will have a strong impact on regenerative medicine. However, as a novel approach, it requires the development of new standard protocols to increase the efficiency of clinical translation. In this review, we summarised the various aspects of requirements related to well‐suited 3D bioprinting techniques for skin regeneration and discussed how to overcome current bottlenecks and propel these therapies into the clinic.

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Section: Cell Selectionmentioning

confidence: 99%

Section: Cell Selectionmentioning

confidence: 99%

“…The laser‐assisted bioprinter is based on laser‐induced forward transfer . Michael et al formulated a mixture comprised of FBs and KCs with hydrocortisone.…”

Section: D Bioprintermentioning

confidence: 99%

Section: Bioinkmentioning

confidence: 99%

Section: Bioinkmentioning

confidence: 99%

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Beyond 2D: 3D bioprinting for skin regeneration

Wang

Yao

et al. 2018

International Wound Journal

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Bioprinting of Human Musculoskeletal Interface

Luo

Wang

et al. 2019

Adv Eng Mater

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Human musculoskeletal interface (MI) refer to biofunctional and engineering similarities enabling smooth connections through muscular and skeletal attachments. MI is commonly involved in musculoskeletal injuries and degenerative diseases, while the key problem to achieve biological integration with the surrounding host tissues of MI is fabricating substitution with precisely structural and material distribution. Bioprinting has made it possible to achieve artificial tissues with spatial controlled heterogeneity of physical properties and bioactive composition similar to native MI tissues. In this review, emphasis is put on detailed introduction of structural and biofunctional properties of MI tissues, as well as recent MI bioprinting application of microextrusion and inkjet bioprinting approaches. Specially, by combining 3D printing and bioprinting approaches, hybrid bioprinting approaches of reinforced MI constructs are retrospected. Other issues of biomaterials, bioprinting methods and relevant regulations are discussed. Future challenges in engineering, material science and clinical transformation are also summarized. Taken together, bioprinting of MI tissues can be of great interest in future development of tissue engineering and regenerative medicine.

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3D Printed Scaffolds Incorporated with Platelet‐Rich Plasma Show Enhanced Angiogenic Potential while not Inducing Fibrosis

Ibanez

Amaral

Simpson

et al. 2021

Adv Funct Materials

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Successful therapeutic strategies for wound healing rely on proper vascularization while inhibiting fibrosis. However, scaffolds designed for skin tissue engineering generally lack the biochemical cues that can enhance their vascularization without inducing fibrosis. Therefore, the objective of this work is to incorporate platelet‐rich plasma (PRP), a natural source of angiogenic growth factors, into a gelatin methacrylate (GelMA) hydrogel, yielding a bioink that can subsequently be used to 3D print a novel regenerative scaffold with defined architecture for skin wound healing. A PRP‐activated bioink is successfully 3D printed, and the resulting scaffolds present similar structural, rheological, and mechanical properties compared to GelMA‐only scaffolds. Furthermore, 3D printed PRP‐activated scaffolds facilitate controlled release of PRP‐derived growth factors for up to 14 days, presenting superior angiogenic potential in vitro (e.g., tubulogenesis assay) and in vivo (chick chorioallantoic membrane) compared to GelMA‐only scaffolds, while not inducing a myofibroblastic phenotype in fibroblasts (e.g., α‐smooth muscle actin expression). This disruptive technology offers the opportunity for a patient's autologous growth factors to be incorporated into a tailored 3D‐printed scaffold in theatre prior to implantation, as part of a single‐stage procedure, and has potential in other tissue engineering applications in which enhanced vascularization with limited fibrosis is desired.

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Skin tissue engineering using 3D bioprinting: An evolving research field

Cited by 156 publications

References 62 publications

Beyond 2D: 3D bioprinting for skin regeneration

Beyond 2D: 3D bioprinting for skin regeneration

Bioprinting of Human Musculoskeletal Interface

3D Printed Scaffolds Incorporated with Platelet‐Rich Plasma Show Enhanced Angiogenic Potential while not Inducing Fibrosis

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