Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule

Metz-Favre, C.; Linhart, Birgit; Focke‐Tejkl, Margarete; Purohit, Ashok; Blay, F. De; Valenta, Rudolf; Pauli, G.

doi:10.1016/j.jaci.2007.03.046

Cited by 27 publications

(25 citation statements)

References 25 publications

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“…Relevant allergens can also be fused in the form of recombinant hybrid molecules which contain the allergens in 1 molecule [44]. In fact, SPTs have shown that grass-pollen-allergic patients were diagnosed with a hybrid molecule consisting of 4 Timothy grass pollen allergens [45]. …”

Section: Discussionmentioning

confidence: 99%

Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial Dermatophagoides pteronyssinus Extracts

Casset¹,

Mari

Purohit

et al. 2012

Int Arch Allergy Immunol

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159

112

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Background: Diagnosis and immunotherapy of house-dust mite (HDM) allergy is still based on natural allergen extracts. The aim of this study was to analyze commercially available Dermatophagoides pteronyssinus extracts from different manufacturers regarding allergen composition and content and whether variations may affect their allergenic activity. Methods: Antibodies specific for several D. pteronyssinus allergens (Der p 1, 2, 5, 7, 10 and 21) were used to analyze extracts from 10 different manufacturers by immunoblotting. Sandwich ELISAs were used to quantify Der p 1 and Der p 2 in the extracts. Mite-allergic patients (n = 45) were skin-tested with the extracts and tested for immunoglobulin E (IgE) reactivity to a panel of 10 mite allergens (Der p 1, 2, 4, 5, 7, 8, 10, 14, 20 and 21) by dot blot. Results: Only Der p 1 and Der p 2 were detected in all extracts but their concentrations and ratios showed high variability (Der p 1: 6.0–40.8 µg ml^–1; Der p 2: 1.7–45.0 µg ml^–1). At least 1 out of 4 allergens (i.e. Der p 5, 7, 10 and 21) was not detected in 8 of the studied extracts. Mite-allergic subjects showed different IgE reactivity profiles to the individual mite allergens, the extracts showed different allergenic activity in skin-prick tests and false-negative results. Conclusions: Commercially available D. pteronyssinus extracts lack important allergens, show great variability regarding allergen composition and content and some gave false-negative diagnostic test results in certain patients.

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Section: Discussionmentioning

confidence: 99%

Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial Dermatophagoides pteronyssinus Extracts

Casset¹,

Mari

Purohit

et al. 2012

Int Arch Allergy Immunol

Self Cite

159

112

View full text Add to dashboard Cite

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“…The pollen families with high allergenicity found in Turkey are Poaceae, Chenopodiaceae, Astraceae, Oleaceae, Cupressaceae and Betulaceae. The Poaceae and Chenopodiaceae/Amaranthaceae families, which comprise the highest percentage of grass and weed taxa, have the highest capacity to induce an allergic immune response in individuals with allergic diseases (D'Amato et al 2007;Metz-Favre et al 2007;Gálan et al 1989;Barderas et al 2007). The months with maximum counts of pollen of the Poaceae and Chenopodiaceae/Amaranthaceae family, i.e., May, June, July and August, are the periods when pollen allergic patients must take precautions.…”

Section: Discussionmentioning

confidence: 99%

Pollen counts and their relationship to meteorological factors in Ankara, Turkey during 2005–2008

et al. 2010

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Pollen plays an important role in the development and exacerbation of allergic diseases. We aimed to investigate the days with highest counts of the most allergenic pollens and to identify the meteorological factors affecting pollen counts in the atmosphere of Ankara, Turkey. Airborne pollen measurements were carried out from 2005 to 2008 with a Burkard volumetric 7-day spore trap. Microscope counts were converted into atmospheric concentrations and expressed as pollen grains/m(3). Meteorological parameters were obtained from the State Meteorological Service. All statistical analyses were done with pollen counts obtained from March to October for each year. The percentages of tree, grass and weed pollens were 72.1% (n = 24,923), 12.8% (n = 4,433) and 15.1% (n = 5,219), respectively. The Pinaceae family from tree taxa (39% to 57%) and the Chenopodiaceae/Amaranthaceae family from weed taxa, contributed the highest percentage of pollen (25% to 43%), while from the grass taxa, only the Poaceae family was detected from 2005 to 2008. Poaceae and Chenopodiaceae/Amaranthaceae families, which are the most allergenic pollens, were found in high numbers from May to August in Ankara. In multiple logistic regression analysis, wind speed (OR = 1.18, CI95% = 1.02-1.36, P = 0.023) for tree pollen, daily mean temperature (OR = 1.10, CI95% = 1.04-1.17, P = 0.001) and sunshine hours (OR = 1.15, CI95% = 1.01-1.30, P = 0.033) for grass pollen, and sunshine hours (OR = 3.79, CI95% = 1.03-13.92, P = 0.044) for weed pollen were found as significant risk factors for high pollen count. The pollen calendar and its association with meteorological factors depend mainly on daily temperature, sunshine hours and wind speed, which may help draw the attention of physicians and allergic patients to days with high pollen counts.

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“…Using this technology, the IgG responses against weakly immunogenic grass pollen allergens such as Phl p 2 and Phl p 6 could be strongly increased when the animals were immunized. A hybrid molecule containing the major grass pollen allergens was found to be equivalent to allergen extracts when used for skin prick testing of allergic patients [113].…”

Section: Past and Present Attempts For The Improvement Of Allergen-spmentioning

confidence: 99%

Developments in allergen‐specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen‐specific immunoglobulin E and T cell reactivity

Focke

Swoboda

Marth

et al. 2010

Clin Experimental Allergy

101

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SummaryAllergen-specific immunotherapy (SIT) is the only specific and disease-modifying approach for the treatment of allergy but several disadvantages have limited its broad applicability. We argue that the majority of the possible disadvantages of SIT such as unwanted effects, poor efficacy and specificity as well as inconvenient application are related to the poor quality of natural allergen extracts, which are the active ingredients of all currently available allergy vaccines. Because of the progress made in the field of molecular allergen characterization, new allergy vaccines based on recombinant allergens, recombinant hypoallergenic allergen derivatives and allergen-derived T cell peptides have entered clinical testing and hold promise to reduce the side-effects and to increase the specificity as well as the efficacy of SIT. Here, we present a refined immunotherapy concept, which is based on the use of peptides derived from allergen surfaces that exhibit reduced, allergen-specific IgE as well as T cell reactivity. These peptides when fused to non-allergenic carriers give rise to allergen-specific protective IgG responses with T cell help from a non-allergenic carrier molecule. We summarize the experimental data demonstrating that such peptide vaccines can bypass allergen-specific IgE as well as T cell activation and may be administered at high doses without IgE-and T cell-mediated side-effects. Should these peptide vaccines prove efficacious and safe in clinical trials, it may become possible to develop convenient, safe and broadly applicable forms of SIT as true alternatives to symptomatic, drug-based allergy treatment.

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Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule

Cited by 27 publications

References 25 publications

Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial <b><i>Dermatophagoides pteronyssinus</i></b> Extracts

Varying Allergen Composition and Content Affects the in vivo Allergenic Activity of Commercial <b><i>Dermatophagoides pteronyssinus</i></b> Extracts

Pollen counts and their relationship to meteorological factors in Ankara, Turkey during 2005–2008

Developments in allergen‐specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen‐specific immunoglobulin E and T cell reactivity

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