Skin Substitutes in Wound Healing and the Stimulatory Effects of Adipose-Derived Stem Cells for the Proliferation of Keratinocytes on Chitosan

Gomathysankar, Sankaralakshmi; Halim, Ahmad Sukari; Makhtar, Wan Ratmaazila Wan; Saad, Arman Zaharil Mat; Yaacob, Nik Soriani

doi:10.1007/15695_2017_104

Cited by 4 publications

(2 citation statements)

References 116 publications

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“…Due to the complex process of wound healing, the full extent of the underlying mechanisms responsible for the effects of chitosan on keratinocytes or fibroblasts are not fully elucidated [ 60 , 67 ]. However, chitosan appears to support granulation and remodeling through its effects on the inflammatory cells and growth factors [ 68 ]. Certain growth factors are important in the migration and proliferation of keratinocytes [ 69 ].…”

Section: Resultsmentioning

confidence: 99%

Chitosomes-In-Chitosan Hydrogel for Acute Skin Injuries: Prevention and Infection Control

et al. 2021

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Burns and other skin injuries are growing concerns as well as challenges in an era of antimicrobial resistance. Novel treatment options to improve the prevention and eradication of infectious skin biofilm-producing pathogens, while enhancing wound healing, are urgently needed for the timely treatment of infection-prone injuries. Treatment of acute skin injuries requires tailoring of formulation to assure both proper skin retention and the appropriate release of incorporated antimicrobials. The challenge remains to formulate antimicrobials with low water solubility, which often requires carriers as the primary vehicle, followed by a secondary skin-friendly vehicle. We focused on widely used chlorhexidine formulated in the chitosan-infused nanocarriers, chitosomes, incorporated into chitosan hydrogel for improved treatment of skin injuries. To prove our hypothesis, lipid nanocarriers and chitosan-comprising nanocarriers (≈250 nm) with membrane-active antimicrobial chlorhexidine were optimized and incorporated into chitosan hydrogel. The biological and antibacterial effects of both vesicles and a vesicles-in-hydrogel system were evaluated. The chitosomes-in-chitosan hydrogel formulation demonstrated promising physical properties and were proven safe. Additionally, the chitosan-based systems, both chitosomes and chitosan hydrogel, showed an improved antimicrobial effect against S. aureus and S. epidermidis compared to the formulations without chitosan. The novel formulation could serve as a foundation for infection prevention and bacterial eradication in acute wounds.

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Section: Resultsmentioning

confidence: 99%

Chitosomes-In-Chitosan Hydrogel for Acute Skin Injuries: Prevention and Infection Control

et al. 2021

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“…The absorbance increases from days 1 to 5 even at higher concentrations (200 μg/mL), indicating cell proliferation. Chitosan binds with serum heparin or growth factors generated by platelets to form polyelectrolyte complexes and brings these components to the cell surface, that stimulate the cell proliferation. − Figure S11 represents the response of melanoma cells (B16F10) to the samples at the same doses and time period. All of the patches display excellent biocompatibility, except at the highest concentration (200 μg/mL).…”

Section: Resultsmentioning

confidence: 99%

Chitosan-TEMPO-Oxidized Nanocellulose Magnetic Responsive Patches with Hyperthermia Potential for Smart Melanoma Therapy

Sumitha,

Krishna,

Sailaja

2023

ACS Appl. Polym. Mater.

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Melanoma treatment is hampered by high metastasis, limited skin penetration of drugs, and insufficient durability of the existing polymer-based drug delivery systems. This study proposes doxorubicin (DOX)-loaded magnetic patches comprising chitosan and TEMPO-oxidized nanocellulose as a potential magnetic drug delivery system. Magnetic patches were prepared by integrating superparamagnetic iron oxide nanoparticles (IONPs) into chitosan-TEMPO-oxidized nanocellulose films and then loaded with DOX. The magnetic, morphological, mechanical, and degradation properties were modulated by varying chitosan-to-IONP ratio and subsequently evaluated for magnetically triggered drug release, hyperthermia, and in vitro biofunctionality. The microtexture of the film surface indicates homogeneous IONP distribution (SEM-EDX) which contributes directly to the high mechanical strength (35–54 MPa) and magnetic sensitivity. These patches are biodegradable and cytocompatible (MTT assay, cell adhesion, and confocal imaging) with a 30–37% greater swelling index at tumor pH (relative to physiological pH) and exhibit superparamagnetism with a saturation magnetization value as high as 23.3 emu/g. The magnetic field-triggered delivery under a static magnetic field of 50 mT produced a release of >12% out of the total loaded amount within 1 h, while >24 h was required to attain the same release level in the absence of magnetic field. As per hyperthermia studies, the drug-loaded magnetic patch elevates the temperature to the therapeutic range (>42 °C) in less than 7 min. The efficacy of DOX-loaded magnetic patches in treating melanoma was evaluated using B16F10 cells, which exhibited a 79.55% reduction in cell viability after 5 days. A facile preparation method, magnetic hyperthermia, controlled chemotherapeutic effect, and limited complexities make them suitable for skin cancer therapeutics.

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Chitosan for Wound Healing in the Light of Skin Tissue Engineering and Stem Cell Research

Chawla

Dutta

2021

Engineering Materials for Stem Cell Regeneration

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Skin Substitutes in Wound Healing and the Stimulatory Effects of Adipose-Derived Stem Cells for the Proliferation of Keratinocytes on Chitosan

Cited by 4 publications

References 116 publications

Chitosomes-In-Chitosan Hydrogel for Acute Skin Injuries: Prevention and Infection Control

Chitosomes-In-Chitosan Hydrogel for Acute Skin Injuries: Prevention and Infection Control

Chitosan-TEMPO-Oxidized Nanocellulose Magnetic Responsive Patches with Hyperthermia Potential for Smart Melanoma Therapy

Chitosan for Wound Healing in the Light of Skin Tissue Engineering and Stem Cell Research

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