Skin Reactions with Bacterial Filtrates of Anhemolytic Streptococcus, Hemolytic Streptococcus and B. Typhosus

Howell, Katharine M.; Corrigan, Marion

doi:10.1093/infdis/42.2.149

Cited by 13 publications

(3 citation statements)

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“…These conclusions are also supported by the detection of HA antibodies to M proteins in most of the adults and only a few of the infants, although the lack of reactivity in infants could also be attributed to diminished immunological responsiveness. Previous studies in humans with streptococcal culture filtrates, cellular extracts, or partially purified M proteins also showed a much higher incidence of cutaneous reactions in adults than in infants and children (17)(18)(19). However, owing to the relative severity of many of the local and systemic reactions encountered by these and other workers (20,21), the present investigation was conducted cautiously and conservatively.…”

Section: Discussionmentioning

confidence: 70%

Antigenicity of the M Proteins of Group a Hemolytic Streptococci

Fox

Wittner

Dorfman

1966

The Journal of Experimental Medicine

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Epidemiological evidence and laboratory experimentation over the past 20 yr supports the conclusion that immunity to group A streptococcal infection is type-specific. Antibodies directed against the M proteins, the antigens responsible for the specificity of the 50 or more serotypes of group A streptococci, are protective by virtue of their opsonic capacity. This subject has been thoroughly reviewed by Lancefield (1). Recent work has demonstrated that several small doses, e. g. 10/~g, of highly purified M proteins induce type-specific bactericidal antibodies in rabbits (2). The success of these latter experiments has been the impetus for this investigation on the development of a streptococcal vaccine for human use. With highly purified M proteins of types 12, 14, and 24, we have examined the degree of cutaneous hypersensitivity and the level of circulating antibodies in adults and infants. These studies have furnished data on the relative tolerance to M proteins and the possible extent of previous exposure to these serotypes. Secondary bactericidal antibody responses have been induced in adults injected with M protein vaccines without provoking untoward local or systemic reactions. I t is the purpose of this report to ex~mlne the feasibility of type-specific immunization of humans against group A streptococcal infections. Materials and MethodsM Protcin.--Hi~hly purified M proteins were prepared from Group A streptococcal cell walls as previously described (3). A slight modification of the chromatographic procedure eliminated traces of nonspecifie antigens. To a column of carboxymethyl cellulose equilibrated with 0.03 u sodium acetate and containing the M protein sample was added 0.1 ~ sodium acetate buffer at pH 5.5. When the efeuent pH reached 5.5, a linear gradient of 0.1 M potassium phosphate buffer of inc~eAslng pH was applied to the column by adding equal volumes of buffers at pH 6.0, 6.5, and 7.0 to a Technicon "Autograd" mixing chamber for the final elution. Minor fractions of nonspedfic protein emerged prior and subsequent to the elution of the main peak of M protein near pH 6.

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Section: Discussionmentioning

confidence: 70%

Antigenicity of the M Proteins of Group a Hemolytic Streptococci

Fox

Wittner

Dorfman

1966

The Journal of Experimental Medicine

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“…of undiluted broth filtrates. Howell and Corrigan (17) (22) found that school children from rural and urban districts might show a variation in positive reactions from 100 per cent to 25 per cent.…”

Section: Methodsmentioning

confidence: 99%

Skin Reactions to Filtrates of Haemolytic Streptococci in Acute and Subacute Nephritis 1

Hansen-Pruss¹,

Longcope²,

O'Brien³

1929

J. Clin. Invest.

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“…Somewhat similar work was done in 1923 by Bristol (3), who performed tests with whole dried streptococci, and found that in 17 individuals with a history of scarlet fever and 31 without there was a positive reaction in 41 and 61 per cent respectively. Both groups are referred to as "fairly normal persons."…”

mentioning

confidence: 54%