Skin Penetration of Epigallocatechin-3-Gallate and Quercetin from Green Tea and &lt;i&gt;Ginkgo biloba&lt;/i&gt; Extracts Vehiculated in Cosmetic Formulations

Belo, Susi Elaine Dal; Gaspar, Lorena Rigo; Campos, Patrícia Maria Berardo Gonçalves Maia; Marty, Jean‐Paul

doi:10.1159/000241299

Cited by 83 publications

(67 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results are in line with previous research, which was EGCG was not penetrated to the receptor medium and simply deposited in the skin. 23 Another most important factor that can affect drug penetration is partition coefficient. 24 When EGCG is entrapped in the vesicle, the coefficient partition of phospholipid is similar with lipid in the stratum corneum.…”

mentioning

confidence: 99%

Novel Transdermal Ethosomal Gel Containing Green Tea (Camellia sinensis L. Kuntze) Leaves Extract: Formulation and In vitro Penetration Study

Ramadon¹,

Wirarti²,

Anwar³

2017

JYP

View full text Add to dashboard Cite

Background: One of the natural products that have many benefits is green tea (Camellia sinensis L.). It can be used to maintain health because of its high antioxidant activity from epigallocatechin gallate (EGCG). However, EGCG has a low ability to penetrate through the skin due to its hydrophilicity. The aim of this study is to improve the penetration of EGCG through the skin by lipid nanovesicle, namely ethosome. Methods: Ethosomes were made using thin layer hydration method. Ethosomes were formulated with different concentration, equal to 1% (F1), 1.5% (F2), and 2% (F3) of EGCG from green tea leaves extract. They were characterized, then a chosen formula would be formulated into a gel dosage form (ethosomal gel). A gel without ethosome (non-ethosomal gel) was prepared as a control. Both of them were evaluated their penetration enhancement ability using Franz diffusion cells with the abdomen skin of Sprague-Dawley rats. Results and Discussion: Based on the results, F1 had a spherical shape, Dv90 129.00 ± 0.00 nm, polydispersity index 0.05 ± 0.00, zeta potential at -62.6 ± 5.05 mV, and the highest entrapment efficiency of 54.39 ± 0.03 %.

show abstract

mentioning

confidence: 99%

Novel Transdermal Ethosomal Gel Containing Green Tea (Camellia sinensis L. Kuntze) Leaves Extract: Formulation and In vitro Penetration Study

Ramadon¹,

Wirarti²,

Anwar³

2017

JYP

View full text Add to dashboard Cite

show abstract

“…Previous studies on percutaneous absorption of EGCG have been performed in vitro (6,(8)(9)(10)(11), while in vivo investigations have been restricted to animals (6,12,13). However, in vitro testing may produce responses different from the in vivo situation due to modifications of the barrier organization of excised skin (17).…”

Section: In Vivo Skin Penetrationmentioning

confidence: 99%

“…2). This effect will increase the EGCG fraction that can partition/diffuse into the underlying viable epidermis, where the catechin should act (8). The enhanced in vivo penetration of EGCG into the lower region of human stratum corneum, provided by the o/w emulsion, could be traced to the presence of formulation excipients that can alter the intercorneocyte lipid matrix organization (i.e., surfactants used as emulsifiers) or fuse with the stratum corneum lipids (i.e., components of the oil phase of the emulsion), thereby facilitating catechin diffusion.…”

Section: In Vivo Skin Penetrationmentioning

confidence: 99%

“…Previous investigations of cutaneous delivery of EGCG have been performed either in vitro on excised animal and human skin (6,(8)(9)(10)(11) or in vivo in mice and rats (6,12,13), and hence they do not reproduce the actual applicative conditions of dermatological products in man. To overcome this drawback, in this study the influence of topical formulations on the percutaneous penetration of EGCG was evaluated in vivo on human volunteers, using the non-invasive tape-stripping technique (14).…”

mentioning

confidence: 99%

See 1 more Smart Citation

In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations

2014

View full text Add to dashboard Cite

(-)-Epigallocatechin-3-gallate (EGCG), the major and the most physiologically active catechin of green tea, has been shown to prevent, in vitro and in vivo, oxidative damage and depletion of antioxidant enzymes caused by exposure to solar UV radiation (1). Moreover, topical treatment with EGCG has been found to decrease the skin inflammatory response due to sun exposure by inhibiting inflammatory leukocyte infiltration and prostaglandin metabolite production (2, 3). In addition, EGCG has been reported to protect against sunlight-induced suppression of the cutaneous immune system and to prevent photoaging of the skin by reducing the expression of matrix metallo-proteinases triggered by solar UV radiation (4, 5). The aim of the study was to examine the effect of topical vehicles on the in vivo human stratum corneum penetration of the antioxidant and skin photoprotective agent (-)-epigallocatechin-3-gallate (EGCG). Model oil-in-water (o/w) emulsion and gel formulations containing 1 % (m/m) EGCG were prepared and subjected to photodegradation studies in order to select excipients that minimize the light instability of EGCG. The optimized emulsion and gel were applied to human volunteers and the EGCG percutaneous permeation was evaluated in vivo by the tape--stripping technique. No significant differences in the percentage of the applied EGCG dose diffused into the stratum corneum were observed between the o/w emulsion (36.1 ± 7.5 %) and gel (35.5 ± 8.1 %) preparations. However, the amount of EGCG permeated into the deeper region of human stratum corneum was significantly larger for the o/w emulsion compared to the gel. Therefore, the emulsion represents a suitable vehicle for topical delivery of EGCG.

show abstract

“…Green tea is apparently capable of providing skin protection against sun damage by quenching the skin with free radicals (12)(13)(14). Human skin penetration and retention of EGCG from green tea extracts has been found sufficient to exert positive effects on the skin (15).…”

mentioning

confidence: 99%

Design and in vivo evaluation of emulgel formulations including green tea extract and rose oil

2013

View full text Add to dashboard Cite

Plant materials with antioxidant and skin anti-aging properties have been widely used in cosmetic products for many years. Among them Camellia sinensis (L.) (green tea) extract is known for its biological and pharmacological activities attributed mainly to green tea catechins, which are polyphenolic compounds present in unfermented dried leaves of C. sinensis (1, 2). A short heating procedure of green tea leaves led to inactivation of polyphenol oxidase, thereby preserving the antioxidant activities of polyphenols. The total content of polyphenols in tea leaves varies from approximately 20 to 40 %, depending on the subspecies of the plant and geographic location (3). The four major polyphenolic catechins present in green tea leaves are (2) Prevention of skin aging and its treatment is an emerging field for development of new formulations in cosmetics. Accordingly, plant extracts with antioxidant properties are beneficial cosmetic ingredients for this purpose. This study was aimed at developing a stable and easily manufactured emulgel including green tea extract and rose oil that is effective on the barrier function and hydration of the skin. An emulgel formulation containing 20 % green tea extract and 5 % rose oil was designed as a result of pre-formulation studies. Physicochemical characterization, in vitro stability studies, in vivo water content of the stratum corneum and transepidermal water loss studies were carried out afterwards. In vivo studies on ten female subjects were evaluated by using non-invasive skin bioengineering techniques. Finally, a cosmetically acceptable, stable and effective emulgel formulation for skin barrier function with good hydrating properties was obtained for skin hydration, protection and anti-aging purposes.

show abstract

Skin Penetration of Epigallocatechin-3-Gallate and Quercetin from Green Tea and <i>Ginkgo biloba</i> Extracts Vehiculated in Cosmetic Formulations

Cited by 83 publications

References 30 publications

Novel Transdermal Ethosomal Gel Containing Green Tea (Camellia sinensis L. Kuntze) Leaves Extract: Formulation and In vitro Penetration Study

Novel Transdermal Ethosomal Gel Containing Green Tea (Camellia sinensis L. Kuntze) Leaves Extract: Formulation and In vitro Penetration Study

In vivo human skin penetration of (–)-epigallocatechin-3-gallate from topical formulations

Design and in vivo evaluation of emulgel formulations including green tea extract and rose oil

Contact Info

Product

Resources

About