Skin mucus proteome map of European sea bass (<i>Dicentrarchus labrax</i>)

Cordero, Héctor; Brinchmann, Monica F.; Cuesta, Alberto; Meseguer, José; Esteban, María Ángeles

doi:10.1002/pmic.201500120

Cited by 94 publications

(105 citation statements)

References 82 publications

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“…Although no differences in this respect were observed between ventral and dorsal skin in the present study, knowledge of constitutive transcript levels in both regions may be useful for saying the susceptibility of specific regions to stressors, as we showed for ventral skin in a previous study regarding the cytokine expression profile [19]. In addition, a previous study in European sea bass identified lyz , casp1 , lei , hsp70 , nkef1 , nkef2 and gst in skin mucus and evaluated their expression in the skin [10], so these molecules seem to be conserved across species.…”

Section: Resultssupporting

confidence: 68%

“…For this reason, we chose molecules previously reported in skin mucus of teleost, such as the immunoglobulins, ighm and ight [31], which are the major components of the adaptive immune system; as well as other important immune-related markers such as lyz , casp1 , lei , hsp70 , nkef1 , nkef2 and gst , which were found at proteomic level in our previous skin mucus studies [10,14]. The greatest differences between dorsal and ventral regions at transcript level were observed in the immunoglobulin specialized in mucosal immunology [32], ight , with lower levels in the ventral skin than in the dorsal region (Fig 6).…”

Section: Resultsmentioning

confidence: 99%

“…The expression of selected genes was analysed with the 2 −ΔCt method [21], which was performed as described elsewhere [10]. Primers are shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…The presence and abundance of enzymes and/or immunologically active molecules, including proteases, antiproteases, esterase, phosphatase alkaline, peroxidase, lysozyme, immunoglobulin M (IgM) or bacteriostatic peptides, have also been demonstrated in gilthead seabream skin mucus [8,9]. Furthermore, the proteome map of skin mucus in several teleosts [10,11], including gilthead seabream [12,13], has been characterized, as well as the proteomic profiling after dietary supplementation and/or stress [14]. However, few classical microscopic studies have been made of fish skin cells [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Dorso-ventral skin characterization of the farmed fish gilthead seabream (Sparus aurata)

et al. 2017

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The skin is the first barrier of defence in fish, protecting against any external stressor and preserving the integrity and homeostasis of the fish body. The aim of this study was to characterise gilthead seabream skin by isolating cells and studying the cell cycle by flow cytometry, to study the skin histology by scanning electron microscopy and the transcription level of some immune-relevant genes by RT-PCR. Furthermore, the results obtained from samples taken from the dorsal and the ventral part of the specimens are compared. No differences were observed in the cell cycle of cells isolated from the dorsal and ventral zones of the skin or in the gene expression of the genes studied in both epidermal zones. However, the epidermis thickness of the ventral skin was higher than that of the dorsal skin, as demonstrated by image analysis using light microscopy. Besides, scanning electron microscopy pointed to a greater cell size and area of microridges in the apical part of the dorsal epidermal cells compared with ventral skin epidermal cells. This study represents a step forward in our knowledge of the skin structure of an important farmed teleost, gilthead seabream, one of the most commonly farmed fish worldwide. Furthermore, for functional characterization, experimental wounds were carried out comparing the wound healing rate between the dorsal and ventral regions of skin over the time. The results showed higher ratio of wound healing in the ventral region, whose wounds were closed after 15 days, compared to dorsal region of skin. Taking into account all together, this study represents a step forward in our knowledge of the skin structure and skin regeneration of an important farmed teleost, gilthead seabream, one of the most commonly farmed fish worldwide.

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Section: Resultssupporting

confidence: 68%

Section: Resultsmentioning

confidence: 99%

“…The expression of selected genes was analysed with the 2 −ΔCt method [21], which was performed as described elsewhere [10]. Primers are shown in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dorso-ventral skin characterization of the farmed fish gilthead seabream (Sparus aurata)

et al. 2017

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“…Despite the fact that this protein was previously identified in skin mucus of gilthead seabream [19], to our knowledge this is the first time that over-expression has been found after any probiotic diet, Pdp11 in this study. In addition, this protein has also reported in skin mucus of teleosts such as European sea bass [18] or Atlantic cod [34] but further studies are needed to clarify its implication in skin mucus.…”

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confidence: 99%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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Differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress, Journal of Proteomics (2015Proteomics ( ), doi: 10.1016Proteomics ( /j.jprot.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E DM A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractGilthead seabream (Sparus aurata L.) is the major cultured fish species in the Mediterranean area. High density stocking causes stress and increases the impact of diseases leading to economic losses. Probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. The probiotic Shewanella putrefaciens, also known as Pdp11, was firstly isolated from the skin of healthy gilthead seabream. Our study focuses on the skin mucus proteome after dietary probiotic Pdp11 intake in fish maintained under normal or overcrowding conditions. 2-DE of skin mucus followed by LC-MS/MS analysis was done for each experimental group and differentially expressed proteins were identified. The results showed differentially expressed proteins especially involved in immune processes, such as lysozyme, complement C3, natural killer cell enhancing factor and nonspecific cytotoxic cell receptor protein 1, whose transcript profiles were studied by qPCR. A consistency between lysozyme protein levels in the mucus and lysozyme mRNA levels in skin were found. Further research is necessary to unravel the implications of skin mucosal immunity on fish welfare and disease. Biological significanceThe present work reveals the proteomic changes, which are taking place in the skin mucus of stressed and non-stressed gilthead seabream after Pdp11 probiotic intake. The study contributes to improve the knowledge on skin mucosal immunology of this relevant farmed fish species.Furthermore, the paper shows for the first time how a suitable proteomic methodology, in this case 2-DE followed by LC-MS/MS is useful to perform a comparative study with a noninvasive technique of skin mucus of gilthead seabream.

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Validation of Salamander Dermal Mucus Swabs as a Novel, Nonlethal Approach for Amphibian Metabolomics and Glutathione Analysis Following Pesticide Exposure

Van Meter,

Glinski,

Wanat

et al. 2024

Enviro Toxic and Chemistry

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Evaluating biomarkers of stress in amphibians is critical to conservation, yet current techniques are often destructive and/or time‐consuming, which limits ease of use. In the present study, we validate the use of dermal swabs in spotted salamanders (Ambystoma maculatum) for biochemical profiling, as well as glutathione (GSH) stress response following pesticide exposure. Thirty‐three purchased spotted salamanders were acclimated to laboratory conditions at Washington College (Chestertown, MD, USA) for 4 weeks. Following acclimation, salamanders were randomly sorted into three groups for an 8‐h pesticide exposure on soil: control with no pesticide, 2,4‐dichlorophenoxyacetic acid (2,4‐D), or chlorpyrifos. Before and after exposure, mucus samples were obtained by gently rubbing a polyester‐tipped swab 50 times across the ventral and dorsal surfaces. Salamanders were humanely euthanized and dissected to remove the brain for acetylcholinesterase and liver for GSH and hepatic metabolome analyses, and a whole‐body tissue homogenate was used for pesticide quantification. Levels of GSH were present in lower quantities on dermal swabs relative to liver tissues for chlorpyrifos, 2,4‐D, and control treatments. However, 2,4‐D exposures demonstrated a large effect size increase for GSH levels in livers (Cohen's d = 0.925, p = 0.036). Other GSH increases were statistically insignificant, and effect sizes were characterized as small for 2,4‐D mucosal swabs (d = 0.36), medium for chlorpyrifos mucosal swabs (d = 0.713), and negligible for chlorpyrifos liver levels (d = 0.012). The metabolomics analyses indicated that the urea cycle, alanine, and glutamate metabolism biological pathways were perturbed by both sets of pesticide exposures. Obtaining mucus samples through dermal swabbing in amphibians is a viable technique for evaluating health in these imperiled taxa. Environ Toxicol Chem 2024;00:1–12. © 2024 SETAC

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Skin mucus proteome map of European sea bass (Dicentrarchus labrax)

Cited by 94 publications

References 82 publications

Dorso-ventral skin characterization of the farmed fish gilthead seabream (Sparus aurata)

Dorso-ventral skin characterization of the farmed fish gilthead seabream (Sparus aurata)

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Validation of Salamander Dermal Mucus Swabs as a Novel, Nonlethal Approach for Amphibian Metabolomics and Glutathione Analysis Following Pesticide Exposure

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