Skin Microflora.

Leyden, James J.; McGinley, Kenneth J.; Nordström, Katrina; Webster, Guy F.

doi:10.1111/1523-1747.ep12468965

Cited by 112 publications

(57 citation statements)

References 91 publications

(16 reference statements)

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“…The development of tools for high throughput DNA sequencing and computational support for the vast data sets thus generated has overcome the limitation of previous culture-based microbiological studies of the human skin (Leyden et al, 1981(Leyden et al, , 1987Kearney et al, 1984;Roth and James, 1988;Chiller et al, 2001), and has renewed interest in the characterization of the human cutaneous microbiome using culture-independent techniques (Dethlefsen et al, 2007;Oakley et al, 2008;Avila et al, 2009;Hamady and Knight, 2009;Nasidze et al, 2009;Peterson et al, 2009;Turnbaugh et al, 2009;Zhang et al, 2009;Qin et al, 2010). Investigators now are using molecular, DNA-based methodologies to assess the microbial populations present in and on human skin (Fredricks, 2001;Paulino et al, 2006Paulino et al, , 2008Gao et al, 2007Gao et al, , 2008Gao et al, , 2010Fierer et al, 2008Fierer et al, , 2010Grice et al, 2008Grice et al, , 2009Costello et al, 2009;Grice and Segre, 2011).…”

Section: Introductionmentioning

confidence: 99%

Distinct cutaneous bacterial assemblages in a sampling of South American Amerindians and US residents

et al. 2012

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The human skin harbors complex bacterial communities. Prior studies showing high inter-individual variation focused on subjects from developed countries. We therefore compared cutaneous bacterial communities of Amerindians in the Venezuelan Amazon with subjects in the United States. Forearm skin specimens were studied from healthy Amerindians in Platanillal village in Amazonas State, and from healthy persons in New York and Colorado. All skin sampling used similar swab/ buffer techniques. Multiplexed V2-targeted 16S rRNA gene pyrosequencing yielded high quality sequences from 112 samples. The results show 20 phyla, with three (Proteobacteria, Firmicutes, Actinobacteria) predominating. US residents and Venezuelan Amerindians had significantly different forearm skin bacterial community compositions, with United States dominated by Propionibacterium. Among the Amerindians, there was a deep split based on bacterial community membership, with 30 and 42 samples, respectively, falling into each of the two groups, not associated with age, gender, or body mass index. One Amerindian group had diversity similar to the United States, but was dominated by Staphylococcus rather than Propionibacterium. The other Amerindian group was significantly more diverse and even than the US or the other Amerindian group, and featured a broad range of Proteobacteria. The results provide evidence that ethnicity, lifestyle and/or geography are associated with the structure of human cutaneous bacterial communities.

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Section: Introductionmentioning

confidence: 99%

Distinct cutaneous bacterial assemblages in a sampling of South American Amerindians and US residents

et al. 2012

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“…Staphylococcus, Micrococcus, Corynebacterium, Brevibacteria, Propionibacteria, and Acinetobacter species, among others, are regularly cultivated from normal skin (3,4). Staphylococcus aureus, Streptococcus pyogenes, and Pseudomonas aeruginosa may be transient colonizers, especially in pathological conditions (5)(6)(7).…”

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confidence: 99%

Molecular analysis of human forearm superficial skin bacterial biota

Gao

Tseng

Pei

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

606

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The microbial ecology of human skin is complex, but little is known about its species composition. We examined the diversity of the skin biota from the superficial volar left and right forearms in six healthy subjects using broad-range small subunit rRNA genes (16S rDNA) PCR-based sequencing of randomly selected clones. For the initial 1,221 clones analyzed, 182 species-level operational taxonomic units (SLOTUs) belonging to eight phyla were identified, estimated as 74.0% [95% confidence interval (C.I.), Ϸ64.8 -77.9%] of the SLOTUs in this ecosystem; 48.0 ؎ 12.2 SLOTUs were found in each subject. Three phyla (Actinobacteria, Firmicutes, and Proteobacteria) accounted for 94.6% of the clones. Most (85.3%) of the bacterial sequences corresponded to known and cultivated species, but 98 (8.0%) clones, comprising 30 phylotypes, had <97% similarity to prior database sequences. Only 6 (6.6%) of the 91 genera and 4 (2.2%) of the 182 SLOTUs, respectively, were found in all six subjects. Analysis of 817 clones obtained 8 -10 months later from four subjects showed additional phyla (numbering 2), genera (numbering 28), and SLOTUs (numbering 65). Only four (3.4%) of the 119 genera (Propionibacteria, Corynebacteria, Staphylococcus, and Streptococcus) were observed in each subject tested twice, but these genera represented 54.4% of all clones. These results show that the bacterial biota in normal superficial skin is highly diverse, with few well conserved and well represented genera, but otherwise low-level interpersonal consensus.human microbial ecology ͉ small subunit rRNA genes ͉ clone library ͉ microbial biota diversity ͉ normal human skin

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“…y corineformes [8][9][10] . En este estudio no se aislaron Micrococcus ni corineformes desde las muestras de piel sana, ni desde las muestras de piel quemada.…”

Section: Discussionunclassified

Colonización bacteriana de piel sana versus quemada de niños bajo 15 años tratados en COANIQUEM, Santiago de Chile

F³

et al. 2012

Rev. chil. infectol.

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Bacterial colonization in areas of burnt skin and healthy children younger than 15 years, seen COANIQUEM, Santiago, ChileBacterial colonization in patients with burns skin without infection and outpatient management has been poorly studied. Objective: To quantify and identify the type of bacteria that colonize healthy body areas homologous location of the skin in children from 1 to 15 years, seen COANIQUEM, Santiago, Chile. Per patient, we studied 204 samples from each zone. The isolated microorganisms were S. epidermidis 35, 3%; others Staphylococcus coagulase negative, 25.8%; S. aureus, 4.4%; and S. viridans group 1.4%. In the subgroup of children who had a count between 1 and 1000 cfu, there was a higher proportion of cfu in burned skin healthy skin (p = 0.0359). The association between depth of the lesion and the bacterial count obtained p = 0.034. Conclusion: In outpatient treatment, the microorganisms are expected in healthy skin, burned skin is evident in lower counts probably associated with epithelial damage.

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Skin Microflora.

Cited by 112 publications

References 91 publications

Distinct cutaneous bacterial assemblages in a sampling of South American Amerindians and US residents

Distinct cutaneous bacterial assemblages in a sampling of South American Amerindians and US residents

Molecular analysis of human forearm superficial skin bacterial biota

Colonización bacteriana de piel sana versus quemada de niños bajo 15 años tratados en COANIQUEM, Santiago de Chile

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