Skin Microbiota in Obese Women at Risk for Surgical Site Infection After Cesarean Delivery

Rood, Kara M.; Buhimschi, Irina A.; Jurcisek, Joseph A.; Summerfield, Taryn; Zhao, Guomao; Ackerman, William E.; Wang, Weiwei; Rumpf, R. Wolfgang; Thung, Stephen; Buhimschi, Catalin S.

doi:10.1038/s41598-018-27134-5

Cited by 27 publications

(32 citation statements)

References 43 publications

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“…DNABII-directed antibodies will also disrupt polymicrobial biofilm fragments (e.g. eDNA+DNABII protein lattices) within clinical specimens recovered from patients with post-tympanostomy tube otorrhea [24], cystic fibrosis [19] and Cesarean section wound infections [23]. Moreover, this approach is proven to be significantly effective pre-clinically in three experimental models of chronic human diseases: otitis media (OM) induced by nontypeable Haemophilus influenzae (NTHI) in the chinchilla [13,21], lung infection due to Pseudomonas aeruginosa in the murine host [21] and in a rat model of Aggregatibacter actinomycetemcomitans-induced periodontal peri-implantitis [18].…”

Section: Introductionmentioning

confidence: 99%

Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections

Novotny

Goodman

2020

eBioMedicine

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Background:: Chronic and recurrent bacterial diseases are recalcitrant to treatment due to the ability of the causative agents to establish biofilms, thus development of means to prevent or resolve these structures are greatly needed. Our approach targets the DNABII family of bacterial DNA-binding proteins, which serve as critical structural components within the extracellular DNA scaffold of biofilms formed by all bacterial species tested to date. DNABII-directed antibodies rapidly disrupt biofilms and release the resident bacteria which promote their subsequent clearance by either host immune effectors or antibiotics that are now effective at a notably reduced concentration. Methods:: First, as a therapeutic approach, we used intact IgG or Fab fragments against a chimeric peptide immunogen designed to target protective epitopes within the DNA-binding tip domains of integration host factor to disrupt established biofilms in vitro and to mediate resolution of existing disease in vivo. Second, we performed preventative active immunisation with the chimeric peptide to induce the formation of antibody that blocks biofilm formation and disease development in a model of viral-bacterial superinfection. Further, toward the path for clinical use, we humanised a monoclonal antibody against the chimeric peptide immunogen, then characterised and validated that it maintained therapeutic efficacy. Findings:: We demonstrated efficacy of each approach in two well-established pre-clinical models of otitis media induced by the prevalent respiratory tract pathogen nontypeable Haemophilus influenzae, a common biofilm disease. Interpretation:: Collectively, our data revealed two approaches with substantive efficacy and potential for broad application to combat diseases with a biofilm component.

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Section: Introductionmentioning

confidence: 99%

Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections

Novotny

Goodman

2020

eBioMedicine

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“…Additionally, there is evidence that exposure to these vaginal microbiota increases the risk of cesarean wound infection. 16 The association between IUPC use and maternal infection provides a possible area for intervention to reduce postoperative morbidity. A recent prospective trial supported the use of prophylactic antibiotics postpartum in obese patients to reduce their risk of postcesarean infection.…”

Section: Discussionmentioning

confidence: 99%

Intrauterine Pressure Catheter Use Is Associated with an Increased Risk of Postcesarean Surgical Site Infections

et al. 2019

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Objective This study aimed to determine if intrapartum placement of an intrauterine pressure catheter (IUPC) is associated with an increased rate of surgical site infections in women undergoing a cesarean delivery. Study Design This was a secondary analysis of the prospective observational Maternal–Fetal Medicine Units Network Vaginal Birth after Cesarean Registry. We compared patients with and without IUPC use. A multivariable logistic regression was performed to evaluate for an association between IUPC use and postcesarean surgical site infections. Results The study included 16,887 women: 7,441 with IUPC use and 9,446 without IUPC use. After adjustment for potential cofounders, IUPC use was associated with an increased risk of postcesarean infections compared with those without IUPC use (adjusted odds ratio: 1.28; 95% confidence interval: 1.10–1.50; p = 0.002). Conclusion IUPC use is associated with an increased risk of postcesarean surgical site infections. This supports the judicious use of IUPC for limited clinical indications and provides a potential area of focus for reduction in postcesarean infections.

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“…Obese pregnant women were found to have a different skin microbiome profile as compared to non-obese pregnant women, and the higher pre-operative bacterial biomass on the skin of obese mothers may confer an increased risk of surgical site infection following caesarean delivery. 65 The effect of environmental exposure on skin microbiome and the dysbiosis of skin microbiome in people with NCDs support a possible role for environmental dust in modulating skin microbiome profiles that are associated with the development of NCDs.…”

Section: Dust and Skin Microbiomementioning

confidence: 99%

Developmental Origins of Health and Disease: Impact of environmental dust exposure in modulating microbiome and its association with non-communicable diseases

Ooi

Tan

Tay

et al. 2020

J Dev Orig Health Dis

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Non-communicable diseases (NCDs) including obesity, diabetes, and allergy are chronic, multi-factorial conditions that are affected by both genetic and environmental factors. Over the last decade, the microbiome has emerged as a possible contributor to the pathogenesis of NCDs. Microbiome profiles were altered in patients with NCDs, and shift in microbial communities was associated with improvement in these health conditions. Since the genetic component of these diseases cannot be altered, the ability to manipulate the microbiome holds great promise for design of novel therapies in the prevention and treatment of NCDs. Together, the Developmental Origins of Health and Disease concept and the microbial hypothesis propose that early life exposure to environmental stimuli will alter the development and composition of the human microbiome, resulting in health consequences. Recent studies indicated that the environment we are exposed to in early life is instrumental in shaping robust immune development, possibly through modulation of the human microbiome (skin, airway, and gut). Despite much research into human microbiome, the origin of their constituent microbiota remains unclear. Dust (also known as particulate matter) is a key determinant of poor air quality in the modern urban environment. It is ubiquitous and serves as a major source and reservoir of microbial communities that modulates the human microbiome, contributing to health and disease. There are evidence that reported significant associations between environmental dust and NCDs. In this review, we will focus on the impact of dust exposure in shaping the human microbiome and its possible contribution to the development of NCDs.

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Skin Microbiota in Obese Women at Risk for Surgical Site Infection After Cesarean Delivery

Cited by 27 publications

References 43 publications

Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections

Targeting a bacterial DNABII protein with a chimeric peptide immunogen or humanised monoclonal antibody to prevent or treat recalcitrant biofilm-mediated infections

Intrauterine Pressure Catheter Use Is Associated with an Increased Risk of Postcesarean Surgical Site Infections

Developmental Origins of Health and Disease: Impact of environmental dust exposure in modulating microbiome and its association with non-communicable diseases

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