Skin Mediated Human Papillomavirus Infection in Breast: A Report of Four Cases

Islam, S. M. Ashiqul; Dasgupta, Hemantika; Basu, Mukta; Roy, Anup; Alam, Neyaz; Mandal, Gautam Kumar; Roychoudhury, Susanta; Panda, Chinmay Kumar

doi:10.32677/ijcr.2018.v04.i02.005

Cited by 2 publications

(4 citation statements)

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“…As per the second mechanism, transmission of HPV can occur to breast from any oral site due to oral sexual practices (46). Third one suggests that the transmission of HPV may occur to breast by nipple or micro-lesion of breast skin due to genitalbreast sexual activity (75,76).…”

Section: Possible Route Of Hpv Infection In Breastmentioning

confidence: 99%

Human papilloma virus (HPV) profiles in breast cancer: future management

Islam¹,

Chakraborty²,

Panda³

2020

Ann Transl Med

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Breast cancer (BC) is frequent among women in worldwide as well as in India. Several studies have reported a wide variation (1.6-86.2%) in the frequency of incidence of human papillomavirus (HPV) infection in BC with high prevalence of high risk HPV16 subtype. HPV infection in breast can occur through different routes like body fluid or by micro-lesion of breast skin from genital/agential sites, though the actual mode of HPV transmission is not yet known in details. Frequent integration and sequence variation with low copy number of HPV16 were seen in this tumour. In addition, high frequencies of methylation in p97 promoter region of HPV16 were evident in this tumour. Novel splice variants of E6/E7 along with other common variants and their protein expression were seen in the tumour. This indicates the importance of HPV in this tumor, its early diagnosis and prognosis. Thus, HPV may be targeted through vaccination to control the disease. However, detailed analysis of HPV associated molecular pathogenesis of BC is warranted for proper therapeutic intervention.

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Section: Possible Route Of Hpv Infection In Breastmentioning

confidence: 99%

Human papilloma virus (HPV) profiles in breast cancer: future management

Islam¹,

Chakraborty²,

Panda³

2020

Ann Transl Med

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“…The protein expression of the genes after cisplatin treatment in the CACX cell lines was analysed by western-blot analysis [Islam et al, 2020]. Whole-cell protein was extracted by sonication with RIPA buffer and quanti ed by Bradford reagent.…”

Section: Treatment Of Chemotherapeutic Drug (Cisplatin) On Cacx Cell ...mentioning

confidence: 99%

“…The list of primary and secondary antibodies used for the experiment are listed in Table S2C. Imaging of the coverslip was performed in a uorescence microscope (Leica DM4000 B, Germany) [Islam et al, 2020] ii. Promoter methylation analysis:…”

Section: Treatment Of Chemotherapeutic Drug (Cisplatin) On Cacx Cell ...mentioning

confidence: 99%

“…Promoter methylation analysis of the key regulatory genes was performed separately in the composite (total) normal cervical epithelium (N = 35) later followed in micro-dissected basal-parabasal/spinous layer of the respective epithelium [Fig S1] and primary cervical lesions (n =109), plasma DNA of CACX samples (n=36) and the CACX cell lines by PCR-based methylation-sensitive restriction analysis (MSRA) using the restriction enzyme Hpa-II (Promega, Fitchburg, WI)) [Islam et al, 2020]. Mock digestion was done for each sample devoid of the enzyme.…”

Section: Promoter Methylation Analysismentioning

confidence: 99%

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Down-regulation of FA-BRCA pathway in the molecular pathogenesis of uterine cervical carcinoma of Indian population: clinical and prognostic implications

Dutta

Pal

Sultana

et al. 2022

Preprint

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Objective: Our study was aimed to understand the importance of FA-BRCA pathway genes in cervical carcinogenesis and their association with the prognosis of the disease. Material and methods: we analysed the molecular profiles of the key regulatory genes of FA-BRCA pathway (BRCA1, BRCA2, FANCC, FANCD2) in 109 cervical lesions at different clinical stages and validated in different bioinformatical analysis as well. The results were next correlated with different clinicopathological parameters. Furthermore, the drug tolerance mechanism of the genes was characterized by treating two CACX cell lines (SiHa and HeLa) in presence of the chemotherapeutic drug cisplatin. Result: Our data showed that the expression pattern (mRNA/Protein) of the genes of FA-BRCA pathway was gradually decreased from normal cervical epithelium to the development of carcinogenesis, also validated in different GEO datasets. Further, in-depth look into the results revealed that genetic (deletion) and epigenetic alterations (promoter methylation) [30 to 55 %] of the genes was strongly correlated with their reduced expression and development of cervical cancer among the patients, resulting in worst 5-year overall survival trend. Incidentally, the prevalence of promoter methylation in both plasma and respective tumour DNA of invasive cervical carcinoma patients implicated its prognostic importance and association with disease recurrence in this study. In continuation of that, our in-vitro study revealed that cisplatin could upregulate the FA-BRCA pathway genes gradually with increasing drug concentrations in the CACX cell lines through promoter hypomethylation due to reduced expression of DNMT1, indicating the intrinsic mechanism of drug tolerance of residual tumour cells of the disease.Conclusion: Our data showed that the inactivation of FA-BRCA pathway was associated with the development and prognosis of CACX. Up-regulation of the pathway genes in presence of cisplatin in the CACX cell lines suggested a plausible mechanism of non-responsiveness to the therapy.

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Skin Mediated Human Papillomavirus Infection in Breast: A Report of Four Cases

Cited by 2 publications

References 0 publications

Human papilloma virus (HPV) profiles in breast cancer: future management

Human papilloma virus (HPV) profiles in breast cancer: future management

Down-regulation of FA-BRCA pathway in the molecular pathogenesis of uterine cervical carcinoma of Indian population: clinical and prognostic implications

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