Skin infection by Mycobacterium marinum – diagnostic and therapeutic challenge

Seidel, Angélica; Nunes, Daniel Holthausen; Fernandes, Camilo; Funchal, Gabriella Di Giunta

doi:10.1016/j.abd.2021.03.013

Cited by 7 publications

(2 citation statements)

References 4 publications

(17 reference statements)

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“…11,12 Mycobacteria are widely distributed in diverse environmental niches; they invade the body through damaged skin and cause a variety of skin diseases such as cutaneous tuberculosis, leprosy, Buruli ulcer, and other cutaneous clinical manifestations. [13][14][15][16][17] However, little is known regarding the immune defence mechanisms of keratinocytes against mycobacteria.…”

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confidence: 99%

Induction of IL‐32 in the immune response of keratinocytes to Mycobacterium marinum infection

Sang

Xue

et al. 2023

Experimental Dermatology

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Keratinocytes are the predominant cell type in the skin epidermis, and they not only protect the skin from the influence of external physical factors but also function as an immune barrier against microbial invasion. However, little is known regarding the immune defence mechanisms of keratinocytes against mycobacteria. Here, we performed single‐cell RNA sequencing (scRNA‐seq) on skin biopsy samples from patients with Mycobacterium marinum infection and bulk RNA sequencing (bRNA‐seq) on M. marinum‐infected keratinocytes in vitro. The combined analysis of scRNA‐seq and bRNA‐seq data revealed that several genes were upregulated in M. marinum‐infected keratinocytes. Further in vitro validation of these genes by quantitative polymerase chain reaction and western blotting assay confirmed the induction of IL‐32 in the immune response of keratinocytes to M. marinum infection. Immunohistochemistry also showed the high expression of IL‐32 in patients' lesions. These findings suggest that IL‐32 induction is a possible mechanism through which keratinocytes defend against M. marinum infection; this could provide new targets for the immunotherapy of chronic cutaneous mycobacterial infections.

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confidence: 99%

Induction of IL‐32 in the immune response of keratinocytes to Mycobacterium marinum infection

Sang

Xue

et al. 2023

Experimental Dermatology

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“…M. marinum gehört zur Familie der nicht‐tuberkulösen Mykobakterien (auch atypische Mykobakterien), tritt als solche ubiquitär und vorwiegend in Süß‐ und Salzwasserhabitaten auf und kann als fakultativ pathogenes Bakterium opportunistische Infektionen beim Menschen hervorrufen [2]. Zur Ansteckung kommt es üblicherweise nach Kontakt mit kontaminiertem Wasser, Fisch oder entsprechenden Fischprodukten [3], insbesondere wenn die Barrierefunktion der Haut gestört ist [4]. Aufgrund dieses Übertragungsweges wird der lokalisierte Befund auch häufig als Schwimmbadgranulom bezeichnet, obwohl dies heutzutage mit einer korrekten Chlorierung von Schwimmbädern kaum noch zu beobachten ist.…”

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