Skin Graft Vascularization Involves Precisely Regulated Regression and Replacement of Endothelial Cells through Both Angiogenesis and Vasculogenesis

Abstract

Replacement of the donor graft vasculature by endothelial and endothelial progenitor cells from the recipient along preexisting channels is the predominant mechanism for skin graft revascularization. This mechanism is likely similar for all nonvascularized free grafts and suggests novel strategies for optimizing the vascularization of tissue constructs engineered in vitro.

Differential transplantability of human endothelial cells in colorectal cancer and renal cell carcinoma primary xenografts

“…22,23 The appearance of these 'BM ghosts' has also been documented in other contexts, such as regression of tumor vessels after inhibition of VEGF signaling, and they have been proposed as potential scaffolds for vessel regrowth. 32 We wanted to know if this event could also take place in the CRC xenograft model.…”

“…22,23 What is more, migration of mouse ECs into the graft was shown to take place over the basement membrane (BM) of previous human vessels. 22,23 In line with these findings, we wanted to know if this event could also be demonstrated in our model of CRC xenograft. To this aim, sections were double-labeled with mAbs antimouse CD34 and antihuman laminin (clone LAM-89).…”

The Effect of Human Recombinant Erythropoietin (rHuEPO) and Tacrolimus (FK506) in Autologous and Homologous Full Thickness Skin Graft (FTSG) Take and Viability in a Rat Model

“…Since it is nearly impossible to assume that simple graft positioning leads to an effective number of successful graft-bed vessel couplings, the aforementioned angiogenetic process has been recently described. Conversely, it has also been suggested that pre-existing channels in the graft allow vessels from the recipient bed to invade the graft from the periphery replacing a considerable number of graft vessels (Capla et al 2006) In order to provide the physiologic mechanism of graft take process it could be suggested that during the initial stages (hours) following transplantation, blood, nutrients and angiogenic factors cover the graft area and bed. Hypoxia along with the substrate of available factors creates the signal for the angiogenic response which commences at 2-3 postgrafting days.…”

Cell Therapy of Critical Limb Ischemia (Problems and Prospects)

“…Показано также, что МСК негемопоэтического происхождения способны трансдифференцироваться в клетки с харак-теристиками эндотелиальных клеток-предшественни-ков [40][41][42][43][44][45] и участвовать в процессах постнатальной неоваскуляризации с помощью паракринных эффектов и секреции ангиогенных факторов [46,47]. Все эти дан-ные положены в основу разработки стратегий терапевти-ческого ангиогенеза и неоваскуляризации при лечении ишемических повреждений [44,[48][49][50][51][52][53].…”