Skin-Derived Precursors Generate Myelinating Schwann Cells for the Injured and Dysmyelinated Nervous System

McKenzie, Ian A.; Biernaskie, Jeff; Toma, Jean G.; Midha, Rajiv; Miller, Freda D.

doi:10.1523/jneurosci.1007-06.2006

Cited by 290 publications

(282 citation statements)

References 34 publications

(72 reference statements)

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“…As a control, both human infant and newborn rat SKPs were maintained for several passages in liquid media dermosphere culture (Fig. 2B), as expected [21,22,24,25,27,35].…”

Section: In Vitro Passage Of Human Skin-derived Precursor (Skp) Cellsmentioning

confidence: 81%

“…Initially derived from rodents, now several groups reported isolation of SKPs from human scalp, foreskin, arm, beard and chin, in subjects ranging from fetal to old age [22,23,30,31,34,[36][37][38][39]. Transplantation of SKPs to animal models of disease has raised expectations for their potential use in human cell therapy [23][24][25][26][27][28][29]. However, translation of results from animal models to a human setting has often resulted in the past in inconsistencies or plain lack of success.…”

Section: Discussionmentioning

confidence: 99%

“…These progenitor cells have been reported to reside in the dermal papillae (DP) of human hair follicles [21,22], their physiological role being unclear at the moment. This easily obtainable multipotent cell population has raised expectations because of their potential use in cell therapy of neurodegeneration [23][24][25][26][27][28][29]. However, we still lack a clear understanding of the spatiotemporal abundance and phenotype of human SKPs.…”

Section: Introductionmentioning

confidence: 99%

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Age-Dependent Depletion of Human Skin-Derived Progenitor Cells

Gago¹,

Pérez-López²,

Sanz-Jaka

et al. 2009

Stem Cells

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A major unanswered question in autologous cell therapy is the appropriate timing for cell isolation. Many of the putative target diseases arise with old age and previous evidence, mainly from animal models, suggests that the stem/progenitor cell pool decreases steadily with age. Studies with human cells have been generally hampered to date by poor sample availability. In recent years, several laboratories have reported on the existence, both in rodents and humans, of skin-derived precursor (SKP) cells with the capacity to generate neural and mesodermal progenies. This easily obtainable multipotent cell population has raised expectations for their potential use in cell therapy of neurodegeneration. However, we still lack a clear understanding of the spatiotemporal abundance and phenotype of human SKPs. Here we show an analysis of human SKP abundance and in vitro differentiation potential, by using SKPs isolated from four distinct anatomic sites (abdomen, breast, foreskin, and scalp) from 102 healthy subjects aged 8 months to 85 years. Human SKP abundance and differentiation potential decrease sharply with age, being extremely difficult to isolate, expand, and differentiate when obtained from the elderly. Our data suggest preserving human SKP cell banks early in life would be desirable for use in clinical protocols in the aging population.

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“…As a control, both human infant and newborn rat SKPs were maintained for several passages in liquid media dermosphere culture (Fig. 2B), as expected [21,22,24,25,27,35].…”

Section: In Vitro Passage Of Human Skin-derived Precursor (Skp) Cellsmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Age-Dependent Depletion of Human Skin-Derived Progenitor Cells

Gago¹,

Pérez-López²,

Sanz-Jaka

et al. 2009

Stem Cells

View full text Add to dashboard Cite

show abstract

“…Thus, the feasibility of acquiring primary human neural crest cells from embryonic tissue is severely challenged by both ethical and technical issues-in fact, neural crest induction and migration is underway long before most women even realize that they are pregnant. Although NCSCs have been isolated from some human adult tissues, they are exceedingly rare and do not possess the same selfrenewal and pluripotency of early embryonic NCSCs [1,11,12]. Therefore, to fully advance in vitro studies of human neural crest development alternate renewable sources of precursor cells will be required.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Neural Crest Stem Cells Derived From In Vitro–Differentiated Human Embryonic Stem Cells

Jiang

Gwye

McKeown

et al. 2009

Stem Cells and Development

132

117

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“…Consequently, attempts to test the therapeutic utility of these highly potent cells are becoming an important research focus. Schwann cells derived from Sox10 Ϫ p75 Ϫ SKPs can both integrate into and generate myelin sheathing in both the PNS and central nervous system (CNS) of shiverer mice (McKenzie et al, 2006), although less success has yet been obtained with SKP-derived neuronal cells (Fernandes et al, 2006). Likewise, initial attempts to get Sox10 ϩ p75 ϩ SKPs to generate cells of the CNS failed even after lesioning, suggesting that these oligopotent cells may not be readily transdifferentiated into CNS cell types (Wong et al, 2006).…”

Section: Skpsmentioning

confidence: 99%

The proliferating field of neural crest stem cells

Delfino‐Machín

Chipperfield

Rodrigues

et al. 2007

Developmental Dynamics

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Neural crest stem cells were first isolated from early embryonic neural crest in the early 1990s, but in the past 5 years, there has been a burst of discoveries of neural crest-derived stem cells from diverse locations. Here, we summarize these data, highlighting the characteristics of each stem cell type. These cells vary widely in the markers they express and the variety of cell types they appear to generate. They occupy diverse locations, but in some cases multiple stem cell types apparently occupy physically proximate niches. To date, few molecular similarities can be identified between these stem cells, although a systematic comparison is required. We note other issues worthy of attention, including aspects of the in vivo behavior of these stem cells, their niches, and their lineage relationships. Together, analysis of these issues will clarify this expanding, but still young, field and contribute to exploration of the important therapeutic potential of these cells. Developmental Dynamics 236:3242-3254, 2007.

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Skin-Derived Precursors Generate Myelinating Schwann Cells for the Injured and Dysmyelinated Nervous System

Cited by 290 publications

References 34 publications

Age-Dependent Depletion of Human Skin-Derived Progenitor Cells

Age-Dependent Depletion of Human Skin-Derived Progenitor Cells

Isolation and Characterization of Neural Crest Stem Cells Derived From In Vitro–Differentiated Human Embryonic Stem Cells

The proliferating field of neural crest stem cells

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