Skin construct or biological bandage?

Scott, Paul; Tredget, E. E.

doi:10.1016/s0140-6736(05)67192-9

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…Phillips et al (2002) reported that the patients treated with a bilayered skin substitute (Apligraf) showed no persistence of allogeneic DNA at 2 months after initial grafting. Our results may explain why the ordinary skin substitutes do not appear to survive permanently after grafting (Scott and Tredget, 2005). These results demonstrate that it might be necessary to increase the ratio of epidermal stem cells in epidermis and the expression of markers in order to create closer resemblance normal skin.…”

Section: Discussionmentioning

confidence: 68%

Enrichment of epidermal stem cells by rapid adherence and analysis of the reciprocal interaction of epidermal stem cells with neighboring cells using an organotypic system

Dong

Liu

et al. 2007

Cell Biology International

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Keratinocytes have the ability to adhere to extracellular matrix rapidly. With this in mind, in this study we isolated keratinocytes known as rapidly adhering (RA) cells. To compare epidermal regenerative abilities, skin substitutes were reconstructed by adding keratinocytes or RA cells to two groups of bioengineered dermis made by fibroblasts and hair follicle dermal cells respectively. After transplantation, the results illustrated that the skin substitutes including RA cells were integrated into the host tissue. Furthermore, with hair follicle dermal cells' influences, the RA cells could form structures very similar to normal hair follicles. These results indicate that RA cells are predominately comprised of epidermal stem cells. The results also demonstrated that besides the reciprocal interaction of epidermal stem cells with dermal cells, the interaction of epidermal stem cells with keratinocytes were critical in epidermis morphogenesis and self-renewal, and application of RA cells could optimize engineering of skin substitutes.

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Section: Discussionmentioning

confidence: 68%

Enrichment of epidermal stem cells by rapid adherence and analysis of the reciprocal interaction of epidermal stem cells with neighboring cells using an organotypic system

Dong

Liu

et al. 2007

Cell Biology International

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“…In the areas where dermis is lost, the newly formed epidermal layer lacks support as it can easily break down under pressure. As a consequence, cells and matrices were integrated in a number of different strategies to introduce bioengineered skin, tissue-engineered skin constructs, bio-synthetic skin substitutes, bio-constructs and other terms [ 21 , 4 ]. These solutions aim to restore the intact barrier that protects the wound from infections, provides pain relief, enables temperature control and prevents fluid loss from the wound surface.…”

Section: Reviewmentioning

confidence: 99%

“…The survival rate became even higher in children, with up to 50% enhanced survivability with 95% burns of TBSA [ 3 ]. The enhanced survival was associated with the emergence of novel solutions for wound coverage that tackle later complications, limit fluid loss, control pain and reduce the risk of hypertrophic scarring [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…The donor-site wound can heal in 2 weeks without apparent scarring. While autologous split-thickness skin grafts are likely to be the treatment of choice in large second-degree and third-degree burns, the lack of suitable donor tissue in the most severely injured patients emphasizes the demand for alternative temporary or permanent wound coverings [ 4 ]. Furthermore, regrafting requires relatively long waiting times for the donor sites and grafted wounds to heal before harvesting additional split-thickness skin [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

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Vascularization is the next challenge for skin tissue engineering as a solution for burn management

et al. 2020

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Skin regeneration represents a promising line of management for patients with skin loss, including burn victims. The current approach of spraying single cells over the defective areas results in variable success rates in different centers. The modern approach is to synthesize a multilayer skin construct that is based on autologous stem cells. One of the main complications with different types of transplants is sloughing due to the absence of proper vascularization. Ensuring proper vascularization will be crucial for the integration of skin constructs with the surrounding tissues. Combination of the right cells with scaffolds of proper physico-chemical properties, vascularization can be markedly enhanced. The material effect, pore size and adsorption of certain proteins, as well as the application of appropriate growth factors, such as vascular endothelial growth factors, can have an additive effect. A selection of the most effective protocols is discussed in this review.

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“…There are two explanations for the results of this study: migration of fetal dermal cells into the wound bed, or that these cells only provided growth factors, chemokines and cytokines by paracrine signaling (Chen et al 2008 ; Gnecchi et al 2008 ; Hocking and Gibran 2010 ). Furthermore, the burn wounds of the children treated with the fetal dermal constructs seemed relatively small, and were possibly not completely full-thickness wounds (Scott and Tredget 2005 ). Therefore, the beneficial effects of dermal substitutes containing fetal dermal cells for the application in full-thickness (burn) wounds remain to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Cell therapy for full-thickness wounds: are fetal dermal cells a potential source?

et al. 2015

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The application of autologous dermal fibroblasts has been shown to improve burn wound healing. However, a major hurdle is the availability of sufficient healthy skin as a cell source. We investigated fetal dermal cells as an alternative source for cell-based therapy for skin regeneration. Human (hFF), porcine fetal (pFF) or autologous dermal fibroblasts (AF) were seeded in a collagen–elastin substitute (Novomaix, NVM), which was applied in combination with an autologous split thickness skin graft (STSG) to evaluate the effects of these cells on wound healing in a porcine excisional wound model. Transplantation of wounds with NVM+hFF showed an increased influx of inflammatory cells (e.g., neutrophils, macrophages, CD4+ and CD8+ lymphocytes) compared to STSG, acellular NVM (Acell-NVM) and NVM+AF at post-surgery days 7 and/or 14. Wounds treated with NVM+pFF presented only an increase in CD8+ lymphocyte influx. Furthermore, reduced alpha-smooth muscle actin (αSMA) expression in wound areas and reduced contraction of the wounds was observed with NVM+AF compared to Acell-NVM. Xenogeneic transplantation of NVM+hFF increased αSMA expression in wounds compared to NVM+AF. An improved scar quality was observed for wounds treated with NVM+AF compared to Acell-NVM, NVM+hFF and NVM+pFF at day 56. In conclusion, application of autologous fibroblasts improved the overall outcome of wound healing in comparison to fetal dermal cells and Acell-NVM, whereas application of fetal dermal fibroblasts in NVM did not improve wound healing of full-thickness wounds in a porcine model. Although human fetal dermal cells demonstrated an increased immune response, this did not seem to affect scar quality.

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Skin construct or biological bandage?

Cited by 7 publications

References 9 publications

Enrichment of epidermal stem cells by rapid adherence and analysis of the reciprocal interaction of epidermal stem cells with neighboring cells using an organotypic system

Enrichment of epidermal stem cells by rapid adherence and analysis of the reciprocal interaction of epidermal stem cells with neighboring cells using an organotypic system

Vascularization is the next challenge for skin tissue engineering as a solution for burn management

Cell therapy for full-thickness wounds: are fetal dermal cells a potential source?

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