Skin cancer treatment effectiveness is improved by iontophoresis of EGFR-targeted liposomes containing 5-FU compared with subcutaneous injection

Petrilli, Raquel; Eloy, Josimar O.; Saggioro, Fabiano Pinto; Chesca, Deise L.; Souza, Marina Claro de; Dias, Maria Assunção Faus da Silva; DaSilva, Luis; Lee, Robert J.; Lopez, Renata Fonseca Vianna

doi:10.1016/j.jconrel.2018.05.038

Cited by 82 publications

(49 citation statements)

References 53 publications

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“…The LBP fraction (5 g) and hydroxyethylcellulose (2.5 g) were mixed and homogenized with ultrapure water (95 mL) resulting in a 5% polysaccharide‐rich hydrogel formulation (LBP). Skin penetration was performed according to a previously described method 20 . In a Franz type vertical diffusion apparatus with a receptor compartment (15 mL) and a diffusion area of 1.1 cm 2 two hydrogel formulations (containing rhodamine B as a penetration marker) were applied to the donor compartment: hydroxyethylcellulose (0.1 g) and rhodamine B (0.4 g) in ultrapure water (4 mL) and hydroxyethylcellulose (0.1 g) and rhodamine B (0.4 g) in 5% LBP fraction (4 mL).…”

Section: Methodsmentioning

confidence: 99%

Polysaccharide‐rich hydrogel formulation combined with photobiomodulation repairs UV‐induced photodamage in mice skin

Neves

Parizotto

Tim

et al. 2020

Wound Repair Regeneration

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Prolonged skin exposure to ultraviolet radiation (UVR) induces premature aging in both the epidermis and the dermis. Chronic exposure to UVR induces the activation of mitogen‐activated protein kinase (MAPK) signaling pathway, activating c‐Jun, c‐Fos expression, and transcription factor of AP‐1 activating protein. AP‐1 activation results in the positive induction of matrix metalloproteinase (MMP) synthesis, which degrade skin collagen fibers. Polysaccharides from the fruit of Lycium barbarum (LBP fraction) have a range of activities and have been demonstrate to repair the photodamage. In different approaches, laser application aims to recover the aged skin without destroying the epidermis, promoting a modulation, called photobiomodulation (PBM), which leads to protein synthesis and cell proliferation, favoring tissue repair. Here we developed a topical hydrogel formulation from a polysaccharide‐rich fraction of Lycium barbarum fruits (LBP). This formulation was associated with PBM (red laser) to evaluate whether the isolated and combined treatments would reduce the UVR‐mediated photodamage in mice skin. Hairless mice were photoaged for 6 weeks and then treated singly or in combination with LBP and PBM. Histological, immunohistochemistry, and immunofluorescence analyses were used to investigate the levels of c‐Fos, c‐Jun, MMP‐1, −2, and −9, collagen I, III, and FGF2. The combined regimen inhibited UVR‐induced skin thickening, decreased the expression of c‐Fos and c‐Jun, as well as MMP‐1, −2, and −9 and concomitantly increased the levels of collagen I, III, and FGF2. The PBM in combination with LBP treatment is a promising strategy for the repair of photodamaged skin, presenting potential clinical application in skin rejuvenation.

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Section: Methodsmentioning

confidence: 99%

Polysaccharide‐rich hydrogel formulation combined with photobiomodulation repairs UV‐induced photodamage in mice skin

Neves

Parizotto

Tim

et al. 2020

Wound Repair Regeneration

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“…2). Among them are immunoliposomes loaded with doxorubicin and cetuximab attached via folate binding protein [161], boron containing anion for neutron cap ture therapy, as well as cholesterol cetuximab [162], cis platin and nimotuzumab [163], 5 fluorouracil and cetux imab [164], liposomes containing zinc phthalocyanine PS and single domain anti EGFR antibody EGa1 [165], liposomes with oxaliplatin and EGF [166], cisplatin and anti EGFR mAb [167], gemcitabine loaded liposomes with attached PEG and anti EGFR mAb [168]. It should be noted that such approaches with targeted delivery of anticancer agents showed an opportunity to overcome multi drug resistance, which often poses problems for cancer therapy [160].…”

Section: The Use Of Egfr As a Delivery Systemmentioning

confidence: 99%

Epidermal Growth Factor Receptor: Key to Selective Intracellular Delivery

Rosenkranz

Slastnikova

2020

Biochemistry Moscow

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Epidermal growth factor receptor (EGFR) is an integral surface protein mediating cellular response to a number of growth factors. Its overexpression and increased activation due to mutations is one of the most common traits of many types of cancer. Development and clinical use of the agents, which block EGFR activation, became a prime example of the personalized targeted medicine. However, despite the obvious success in this area, cancer cure remains unattainable in most cases. Because of that, as well as the result of the search for possible ways to overcome the difficulties of treatment, a huge number of new treatment methods relying on the use of EGFR overexpression and its changes to destroy cancer cells. Modern data on the structure, functioning, and intracellular transport of EGFR, its natural ligands, as well as signaling cascades triggered by the EGFR activation, peculiarities of the EGFR expression and activation in oncological disorders, as well as applied therapeutic approaches aimed at blocking EGFR signaling pathway are summarized and analyzed in this review. Approaches to the targeted delivery of various chemotherapeutic agents, radionuclides, immunotoxins, photosensitizers, as well as the prospects for gene therapy aimed at cancer cells with EGFR overexpression are reviewed in detail. It should be noted that increasing attention is being paid nowadays to the development of multifunctional systems, either carrying several different active agents, or possessing several environment-dependent transport functions. Potentials of the systems based on receptor-mediated endocytosis of EGFR and their possible advantages and limitations are discussed.

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“…This study showed that cell uptake increases proportionally with incubation time. 95 Cell uptake was analyzed by Petrilli et al 96 with confocal microscopy and flow cytometry in cell lines A431 and B16F10 treated with liposomes and immunoliposomes loaded with 3,3′-dioctadecyloxacarbocyanine perchlorate. They showed that immunoliposomes are easier to capture by the cells than simple liposomes.…”

Section: Cell Uptakementioning

confidence: 99%

<p>Development, Characterization and Use of Liposomes as Amphipathic Transporters of Bioactive Compounds for Melanoma Treatment and Reduction of Skin Inflammation: A Review</p>

Castañeda-Reyes

Perea-Flores

Dávila-Ortíz

et al. 2020

IJN

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The skin is the largest organ in the human body, providing a barrier to the external environment. It is composed of three layers: epidermis, dermis and hypodermis. The most external epidermis is exposed to stress factors that may lead to skin conditions such as photo-aging and skin cancer. Some treatments for skin disease utilize the incorporation of drugs or bioactive compounds into nanocarriers known as liposomes. Liposomes are membranes whose sizes range from nano to micrometers and are composed mostly of phospholipids and cholesterol, forming similar structures to cell membranes. Thus, skin treatments with liposomes have lower toxicity in comparison to traditional treatment routes such as parenteral and oral. Furthermore, addition of edge activators to the liposomes decreases the rigidity of the bilayer structure making it deformable, thereby improving skin permeability. Liposomes are composed of an aqueous core and a lipidic bilayer, which confers their amphiphilic property. Thus, they can carry hydrophobic and hydrophilic compounds, even simultaneously. Current applications of these nanocarriers are mainly in the cosmetic and pharmaceutic industries. Nevertheless, new research has revealed promising results regarding the effectiveness of liposomes for transporting bioactive compounds through the skin. Liposomes have been well studied; however, additional research is needed on the efficacy of liposomes loaded with bioactive peptides for skin delivery. The objective of this review is to provide an up-to-date description of existing techniques for the development of liposomes and their use as transporters of bioactive compounds in skin conditions such as melanoma and skin inflammation. Furthermore, to gain an understanding of the behavior of liposomes during the process of skin delivery of bioactive compounds into skin cells.

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Skin cancer treatment effectiveness is improved by iontophoresis of EGFR-targeted liposomes containing 5-FU compared with subcutaneous injection

Cited by 82 publications

References 53 publications

Polysaccharide‐rich hydrogel formulation combined with photobiomodulation repairs UV‐induced photodamage in mice skin

Polysaccharide‐rich hydrogel formulation combined with photobiomodulation repairs UV‐induced photodamage in mice skin

Epidermal Growth Factor Receptor: Key to Selective Intracellular Delivery

<p>Development, Characterization and Use of Liposomes as Amphipathic Transporters of Bioactive Compounds for Melanoma Treatment and Reduction of Skin Inflammation: A Review</p>

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