Skin biopsy analysis reveals predisposition to stretch mark formation

Mitts, Thomas F.; Jimenez, Felipe; Hinek, Aleksander

doi:10.1016/j.asj.2005.09.004

Cited by 38 publications

(41 citation statements)

References 29 publications

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“…It has also been reported that exposing human skin to extreme hyperthermia (43°C) results in accumulation of amorphous elastin aggregates resembling those observed in photo-aged skin (31)(32)(33). In contrast, the results of our study indicate for the first time that exposure to 39 or 41°C induces significant enhancement in the deposition of elastic fibers in cultures of normal aortic SMCs, as well as in cultures of dermal fibroblasts derived from normal human skin and from patients affected by an acquired idiopathic elastinopathy that has caused stretch marks (26,27,41,42) or the secondary elastinopathy observed in genetic CS. Because such a mild hyperthermia did not induce up-regulation in the deposition of fibronectin or collagen type I (Fig.…”

Section: Discussioncontrasting

confidence: 54%

“…Organ Cultures of Explants Derived from Human SkinFragments of normal and stretch-marked skin (26,27) collected during plastic surgery procedures from the stretchmarked skin of three female patients, cases 1, 2, and 3, were cut into multiple 4-mm 2 pieces, placed in culture dishes, and maintained for 5 days at 37 or 41°C in DMEM containing 5% FBS. At the end of this period, all organ cultures were fixed in 1% buffered formalin, and their transverse serial histological sections were stained with Movat's pentachrome (28), which shows elastin as black, glycosaminoglycans as green, collagen as yellow, and cell nuclei as dark blue.…”

Section: Methodsmentioning

confidence: 99%

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The Hyperthermia-enhanced Association between Tropoelastin and Its 67-kDa Chaperone Results in Better Deposition of Elastic Fibers

Murphy

Bunda

Mitts

et al. 2010

Journal of Biological Chemistry

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The results of our in vitro experiments indicate that exposing cultured human aortic smooth muscle cells and dermal fibroblasts to 39 to 41°C induces a significant up-regulation in the net deposition of elastic fibers, but not of collagen I or fibronectin, and also decreases the deposition of chondroitin sulfate-containing moieties. We further demonstrate that mild hyperthermia also rectifies the insufficient elastogenesis notable in cultures of fibroblasts derived from the stretch-marked skin of adult patients and in cultures of dermal fibroblasts from children with Costello syndrome, which is characterized by the accumulation of chondroitin 6-sulfate glycosaminoglycans that induce shedding and inactivation of the 67-kDa elastin-binding protein. We have previously established that this protein serves as a reusable chaperone for tropoelastin and that its recycling is essential for the normal deposition of elastic fibers. We now report that hyperthermia not only inhibits deposition of chondroitin 6-sulfate moieties and the consequent preservation of elastin-binding protein molecules but also induces their faster recycling. This, in turn, triggers a more efficient preservation of tropoelastin, enhancement of its secretion and extracellular assembly into elastic fibers. The presented results encourage using mild hyperthermia to restore elastic fiber production in damaged adult skin and to enhance elastogenesis in children with genetic elastinopathies.

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Section: Discussioncontrasting

confidence: 54%

Section: Methodsmentioning

confidence: 99%

The Hyperthermia-enhanced Association between Tropoelastin and Its 67-kDa Chaperone Results in Better Deposition of Elastic Fibers

Murphy

Bunda

Mitts

et al. 2010

Journal of Biological Chemistry

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“…These lesions closely resemble those seen in patients with Cushing's syndrome (Summers et al, 1964) or patients persistently treated with steroids (Chernosky and Knox, 1964). Stretch marks are characterized by a thin epidermis, loss of dermal papillae, loss of rete ridges (Zheng et al, 1985), and a decrease in the net content of collagen, fibronectin (Lee et al, 1994), fibrillin 1 (Watson et al, 1998), and elastic fibers (Mitts et al, 2005). Especially severe and extended loss of elasticity occurs after enzymatic degradation of the elastic fibers in mid-dermal elastolysis (Patroi et al, 2003), elastosis perforans serpiginosa, and cutis marmorata teleangiectasia congenita (Hinek et al, 2008).…”

Section: Introductionmentioning

confidence: 82%

Aldosterone and Mineralocorticoid Receptor Antagonists Modulate Elastin and Collagen Deposition in Human Skin

Mitts

Bunda

Wang

et al. 2010

Journal of Investigative Dermatology

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We have shown that the steroid hormone aldosterone, recognized for its action on the kidney and the cardiovascular system, also modulates deposition of extracellular matrix in human skin. We have shown that treatment of primary cultures of normal skin fibroblasts with aldosterone (10 n-1 μM), in addition to stimulation of collagen type I expression, induces elastin gene expression and elastic fiber deposition. We have further shown that the elastogenic effect of aldosterone, which can be enhanced in the presence of mineralocorticoid receptor (MR) antagonists spironolactone and eplerenone, is executed in a MR-independent manner via amplification of IGF-I receptor-mediated signaling. Because aldosterone applied alone stimulates both collagen and elastin deposition in cultures of fibroblasts and in cultures of skin explants derived from dermal stretch marks, we postulate that this steroid should be used in the treatment of damaged skin that loses its volume and elasticity. Moreover, aldosterone applied in conjunction with spironolactone or eplerenone induces matrix remodeling and exclusively enhances elastogenesis in cultures of fibroblasts and explants derived from dermal scars and keloids. We therefore propose that intra-lesional injection of these factors should be considered in therapy for disfiguring dermal lesions and especially in prevention of their recurrence after surgical excision.

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“…Another hypothesis suggests that the formation of SD is related to the reorganization and diminution of dermal elastic fibers network, rather than collagen transformation 13. Excessive mechanical stretching of the skin determines the rupture of the elastic fibers while the fibroblasts are unable to repair the components of the extracellular matrix 14. The quality of fibroblasts is also altered in striae, with a reduction in fibronectin, and type I and type III procollagen expression in fibroblasts from SD 8.…”

Section: Introductionmentioning

confidence: 99%

Improvement of skin condition in striae distensae: development, characterization and clinical efficacy of a cosmetic product containing <em>Punica granatum</em> seed oil and <em>Croton lechleri</em> resin extract

Bogdan¹,

Iurian

Tomuță

et al. 2017

DDDT

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Striae distensae are a frequent skin condition associated with pregnancy, weight change or lack of skin elasticity. The aim of this research was to obtain a topical product containing herbal active ingredients with documented antioxidant and anti-inflammatory activity (Punica granatum seed oil and Croton lechleri resin extract) and demonstrate its positive effect on prevention and treatment of striae distensae. First, the cream base formulation was optimized through experimental design. Secondly, the cream containing the two active ingredients was investigated in an interventional nonrandomized clinical trial. The clinical outcome was assessed through biophysical parameters and ultrasonographic evaluation. The state of the skin was evaluated by biophysical measurements and ultrasonography at the beginning of the study and after 3 and 6 weeks. The experimental design was successfully used to set the best ranges for the technological and formulation factors to obtain a cosmetic formulation with optimal characteristics. The study of clinical efficacy on the optimal formulation revealed an increase in the dermis thickness, hydration and elasticity values in both groups after 6 weeks of cream application. The new oil-in-water cream containing P. granatum seed oil and C. lechleri resin extract can be helpful in the prevention or improving of skin changes associated with striae.

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Skin biopsy analysis reveals predisposition to stretch mark formation

Cited by 38 publications

References 29 publications

The Hyperthermia-enhanced Association between Tropoelastin and Its 67-kDa Chaperone Results in Better Deposition of Elastic Fibers

The Hyperthermia-enhanced Association between Tropoelastin and Its 67-kDa Chaperone Results in Better Deposition of Elastic Fibers

Aldosterone and Mineralocorticoid Receptor Antagonists Modulate Elastin and Collagen Deposition in Human Skin

Improvement of skin condition in striae distensae: development, characterization and clinical efficacy of a cosmetic product containing <em>Punica granatum</em> seed oil and <em>Croton lechleri</em> resin extract

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