Skin Bioengineering: Preclinical and Clinical Applications

Martínez-Santamaría, Lucía; Guerrero-Aspizua, Sara; Río, Marcela Del

doi:10.1016/j.adengl.2011.03.016

Cited by 30 publications

(22 citation statements)

References 31 publications

(20 reference statements)

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“…Since the development of iPSCs (Takahashi et al 2007), fibroblasts have been largely used in modeling diseases (Freitas et al 2012;Martinez-Santamaria et al 2012;Marchetto et al 2010;Dimos et al 2008;Park et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Fibroblast sources: Where can we get them?

et al. 2014

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Fibroblasts are cells widely used in cell culture, both for transient primary cell culture or permanent as transformed cell lines. Lately, fibroblasts become cell sources for use in disease modeling after cell reprogramming because it is easily accessible in the body. Fibroblasts in patients will maintain all genetic background during reprogramming into induced pluripotent stem cells. In spite of their large use, fibroblasts are obtained after an invasive procedure, a superficial punch skin biopsy, collected under patient's local anesthesia. Taking into consideration the minimum patient's discomfort during and after the biopsy procedure, as well as the aesthetics aspect, it is essential to reflect on the best site of the body for the biopsy procedure combined with the success of getting robust fibroblast cultures in the lab. For this purpose, we compared the efficiency of four biopsy sites of the body (skin from eyelid, back of the ear, abdominal cesarean scar and groin). Cell proliferation assays and viability after cryopreservation were measured. Our results revealed that scar tissue provided fibroblasts with higher proliferative rates. Also, fibroblasts from scar tissues presented a higher viability after the thawing process.

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Section: Discussionmentioning

confidence: 99%

Fibroblast sources: Where can we get them?

et al. 2014

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“…Then, the extracted mouse skin is devitalized (by several cycles of freezing and thawing) and placed over the implant to ensure its protection during the engraftment process [3]. However, although in this manner the grafted tissue is protected, engraftment cannot readily be assessed as it is covered by the abovementioned devitalized skin.…”

Section: Introductionmentioning

confidence: 99%

Remote diffuse reflectance spectroscopy sensor for tissue engineering monitoring based on blind signal separation

Martín-Mateos¹,

Crespo-Garcia²,

Ruiz-Llata³

et al. 2014

Biomed. Opt. Express

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Abstract:In this study the first results on evaluation and assessment of grafted bioengineered skin substitutes using an optical Diffuse Reflectance Spectroscopy (DRS) system with a remote optical probe are shown. The proposed system is able to detect early vascularization of skin substitutes expressing the Vascular Endothelial Growth Factor (VEGF) protein compared to normal grafts, even though devitalized skin is used to protect the grafts. Given the particularities of the biological problem, data analysis is performed using two Blind Signal Separation (BSS) methods: Principal Component Analysis (PCA) and Independent Component Analysis (ICA). These preliminary results are the first step towards point-of-care diagnostics for skin implants early assessment.

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“…Reconstructed skin models have augmented research analysis involving the cellular and immunological elements of psoriasis, the study of skin pigmentation, skin melanoma, wound healing and allergens with greater flexibility, increased convenience, good reproducibility, and reduced costs. [39][40][41][42] Genetic modification of cultured skin grafts can act as vehicles for cutaneous gene therapy in conditions such as epidermolysis bullosa [43] and Netherton syndrome. [44] CHALLENGES CHALLENGES…”

Section: Vitiligomentioning

confidence: 99%

Skin substitutes in dermatology

Anish¹

2015

Indian J Dermatol Venereol Leprol

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Skin Bioengineering: Preclinical and Clinical Applications

Cited by 30 publications

References 31 publications

Fibroblast sources: Where can we get them?

Fibroblast sources: Where can we get them?

Remote diffuse reflectance spectroscopy sensor for tissue engineering monitoring based on blind signal separation

Skin substitutes in dermatology

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