Skin barrier defect in atopic dermatitis: increased permeability of the stratum corneum using dimethyl sulfoxide and theophylline

Yoshiike, Takashi; Aikawa, Yosuke; Sindhvananda, Jirot; Suto, Hajime; Nishimura, Kiyokazu; Kawamoto, Terufumi; Ogawa, Hideoki

doi:10.1016/0923-1811(93)90076-2

Cited by 78 publications

(53 citation statements)

References 13 publications

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“…About 80% of AD patients have increased amounts of total IgE [3]. Although the precise pathogenic mechanisms of AD are as yet unknown, evidence suggests that immunological abnormalities, such as type I and type IV allergy [3], barrier dysfunction [4, 5, 6, 7], environmental factors and psychotic factors, contribute to the development and pathogenesis of AD. To further explore the pathophysiology and treatment of AD, a suitable animal model is required.…”

Section: Introductionmentioning

confidence: 99%

NC/Nga Mice: A Mouse Model for Atopic Dermatitis

Suto

Matsuda

Mitsuishi

et al. 1999

Int Arch Allergy Immunol

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220

107

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Atopic dermatitis (AD) is a common pruritic disease that occurs primarily in infancy and childhood. AD is characterized by itching and the patient having an individual or family history of atopic diseases. Although AD is also frequently associated with elevated serum IgE levels and with common environmental factors contributing to its pathogenesis, the etiology of AD is still unknown. We examined NC/Nga mice (NC mice) that showed AD-like skin lesions with aging as a possible mouse model for AD. NC mice were maintained under conventional (Conv) or specific pathogen-free (SPF) conditions. Clinical symptoms, serum IgE levels and histopathology of the skin were compared between these 2 groups, and we explored their application as a model of human AD. It was found that the skin lesions of inbred NC mice were clinically and histologically very similar to human AD when the mice were raised under Conv conditions, but not under SPF conditions, and we assumed that some kinds of environmental factors might trigger AD-like signs and symptoms in NC mice. To further investigate the pathophysiology and treatment of AD, a suitable animal model is absolutely required, and NC mice are very useful for this purpose.

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Section: Introductionmentioning

confidence: 99%

NC/Nga Mice: A Mouse Model for Atopic Dermatitis

Suto

Matsuda

Mitsuishi

et al. 1999

Int Arch Allergy Immunol

Self Cite

220

107

View full text Add to dashboard Cite

show abstract

“…These fi ndings were the starting point of the present study, in which we performed a detailed analysis on CER composition, focusing in particular on the CER chain length distribution in nonlesional SC of AE patients in relation to lipid organization and skin barrier dysfunction. We studied SC of nonlesional skin, as we aimed to monitor the changes in lipid properties in the skin barrier function in lesional and nonlesional skin (12)(13)(14)(15)(16).…”

mentioning

confidence: 99%

Increase in short-chain ceramides correlates with an altered lipid organization and decreased barrier function in atopic eczema patients

Janssens

Smeden

Gooris

et al. 2012

Journal of Lipid Research

387

433

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“…Although the passive movement of the dye through the skin may be different to the movement of a larger, motile bacterium, the basic permeability of the epidermis does appear to be a valid representation of the skin barrier function, no matter what particle is used to test it. For example, the skin permeability of humans with atopic dermatitis has been measured using a number of different methods and all of them have found similar results (Yoshiike et al, 1993;Sakurai et al, 2002;Jakasa et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

The effect of slurry on skin permeability to methylene blue dye in dairy cows with and without a history of digital dermatitis

Palmer

Donnelly

Garland

et al. 2013

Animal

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This study aimed to determine whether there was a difference in skin permeability to methylene blue dye or skin morphology between dairy cows that differed in their susceptibility to digital dermatitis (DD) and to assess the effect of contact with slurry on skin permeability. Twenty nine dairy cows were monitored for DD during the winter housing period and classed as DD1 (previous DD infection, n 5 17), or DD2 (no recorded infection, n 5 12). The animals were culled and a skin sample was taken from above the heel of each hind foot and frozen. Samples were later defrosted and one sample from each cow was tested for permeability, whereas the other was treated with slurry for 24 h before permeability testing. To test permeability, methylene blue dye was applied to the skin surface in a Franz diffusion cell. After 48 h, the amount of dye that had passed through the skin was estimated. The stratum corneum thickness and the density of hair follicles were determined from additional heel skin samples. Skin permeability to methylene blue dye was significantly greater for samples that had been treated with slurry but did not differ between DD1 and DD2 animals. No difference was found in the stratum corneum thickness or density of hair follicles between DD1 and DD2 animals. These findings imply that individual differences in general skin permeability are not a major factor in determining DD susceptibility and suggest that contact with slurry could contribute to DD infection by increasing the permeability of the skin, which may facilitate pathogen entry. Further work is required to clarify the role played by slurry in the pathogenesis of DD.

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Skin barrier defect in atopic dermatitis: increased permeability of the stratum corneum using dimethyl sulfoxide and theophylline

Cited by 78 publications

References 13 publications

NC/Nga Mice: A Mouse Model for Atopic Dermatitis

NC/Nga Mice: A Mouse Model for Atopic Dermatitis

Increase in short-chain ceramides correlates with an altered lipid organization and decreased barrier function in atopic eczema patients

The effect of slurry on skin permeability to methylene blue dye in dairy cows with and without a history of digital dermatitis

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