Skin autofluorescence (a marker for advanced glycation end products) and erectile dysfunction in diabetes

Kouidrat, Youssef; Zaitouni, Ahmad A; Amad, Ali; Desailloud, Rachel; Loas, Gwenolé; Lalau, Jean–Daniel

doi:10.1016/j.jdiacomp.2016.10.026

Cited by 11 publications

(12 citation statements)

References 38 publications

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“…Our model shows that exposure to glyoxal leads to the accumulation of lipid droplets in fibroblasts, with impaired proliferative and migratory capacities (6). During the remodeling phase, pro-collagen I is produced correctly (7) but it does not mature properly and it cannot induce the formation of new collagen fibers (8). Glycation is one of the main factors involved in impaired wound healing in patients with diabetes.…”

Section: Discussionmentioning

confidence: 99%

“…5 Thus, diabetes results in the accumulation of AGEs in skin as collagen-CML which may contribute to the disruption of skin integrity, as indicated by the high rate of impaired wound healing in patients with diabetes. [8][9][10] A wound that do not heal after 3 months is called a chronic wound. This generally occurs when the inflammatory, the proliferative, and the remodeling phases of the wound healing process are prolonged and/or altered.…”

Section: Significance Of This Studymentioning

confidence: 99%

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Glycation by glyoxal leads to profound changes in the behavior of dermal fibroblasts

Guillon

Ferraro

Clément

et al. 2021

BMJ Open Diab Res Care

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IntroductionDiabetes is a worldwide health problem that is associated with severe complications. Advanced Glycation End products (AGEs) such as Nε-(carboxymethyl)lysine, which result from chronic hyperglycemia, accumulate in the skin of patients with diabetes. The effect of AGEs on fibroblast functionality and their impact on wound healing are still poorly understood.Research design and methodsTo investigate this, we treated cultured human fibroblasts with 0.6 mM glyoxal to induce acute glycation. The behavior of fibroblasts was analyzed by time-lapse monolayer wounding healing assay, seahorse technology and atomic force microscopy. Production of extracellular matrix was studied by transmission electronic microscopy and western blot. Lipid metabolism was investigated by staining of lipid droplets (LDs) with BODIPY 493/503.ResultsWe found that the proliferative and migratory capacities of the cells were greatly reduced by glycation, which could be explained by an increase in fibroblast tensile strength. Measurement of the cellular energy balance did not indicate that there was a change in the rate of oxygen consumption of the fibroblasts. Assessment of collagen I revealed that glyoxal did not influence type I collagen secretion although it did disrupt collagen I maturation and it prevented its deposition in the extracellular matrix. We noted a pronounced increase in the number of LDs after glyoxal treatment. AMPK phosphorylation was reduced by glyoxal treatment but it was not responsible for the accumulation of LDs.ConclusionGlyoxal promotes a change in fibroblast behavior in favor of lipogenic activity that could be involved in delaying wound healing.

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Section: Discussionmentioning

confidence: 99%

Section: Significance Of This Studymentioning

confidence: 99%

Glycation by glyoxal leads to profound changes in the behavior of dermal fibroblasts

Guillon

Ferraro

Clément

et al. 2021

BMJ Open Diab Res Care

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“…The SAF principle relies on the relation between skin fluorescence and the presence of AGEs ( Figure 5 ) [ 108 ], explaining its application as a marker to assess the chronic complications of DM [ 113 , 114 , 115 , 116 , 117 ]. Considering that skin collagen’s half-life is about 10–15 years [ 118 ], the AGEs bound to skin collagen throughout such a time are a medium to represent the maintained glycemia during long periods [ 113 ].…”

Section: Measurement Of Skin Fluorescencementioning

confidence: 99%

Advanced Glycation End Products: New Clinical and Molecular Perspectives

Salazar

Navarro

Ortega

et al. 2021

IJERPH

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Diabetes mellitus (DM) is considered one of the most massive epidemics of the twenty-first century due to its high mortality rates caused mainly due to its complications; therefore, the early identification of such complications becomes a race against time to establish a prompt diagnosis. The research of complications of DM over the years has allowed the development of numerous alternatives for diagnosis. Among these emerge the quantification of advanced glycation end products (AGEs) given their increased levels due to chronic hyperglycemia, while also being related to the induction of different stress-associated cellular responses and proinflammatory mechanisms involved in the progression of chronic complications of DM. Additionally, the investigation for more valuable and safe techniques has led to developing a newer, noninvasive, and effective tool, termed skin fluorescence (SAF). Hence, this study aimed to establish an update about the molecular mechanisms induced by AGEs during the evolution of chronic complications of DM and describe the newer measurement techniques available, highlighting SAF as a possible tool to measure the risk of developing DM chronic complications.

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“…16 To date, only Kouidrat et al studied the relationship between AGE accumulation and ED in a limited sample of individuals with T1DM. 17 They found that skin autofluorescence (AF), an indirect marker for AGE, is associated with the presence of severe ED. 17…”

Section: Introductionmentioning

confidence: 99%

Erectile Dysfunction in Individuals with Type 1 Diabetes is Associated with Long-term Metabolic Control and Diabetic Complications: A Cross-Sectional Study

et al. 2022

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Background Erectile dysfunction (ED) affects approximately 38% of individuals with type 1 diabetes (T1DM). Skin autofluorescence (AF) reflects skin advanced glycation end product (AGE) deposits and is a marker of long-term glycemia control. Objective The study investigates the relationship between ED and diabetes control in patients with T1DM. Methods Adult patients with T1DM visiting the Diabetology Department were cross-sectionally investigated. Medical history, anthropometric features, and laboratory findings were collected. All individuals filled the International Index of Erectile Function (IIEF-5). IIEF-5 total score < 22 represented the presence of ED. AF was measured on the volar aspect of the forearm using AGE Reader. Insulin resistance (IR) was assessed by the estimated glucose disposal rate. Descriptive statistics and multivariate logistic regression analyses were performed. The adjusted covariates were general risk factors of ED. Results Of a total of n = 70 patients, n = 30 (42.9%) suffered from ED. The presence of ED was associated with higher glycated hemoglobin level (OR, 95% CI; 1.62, 1.02–2.60; p = 0.043), presence of at least one diabetic complication (3.49, 1.10–11.03; p = 0.03), and skin AF (9.20, 1.60–52.94; p = 0.01), but not with IR (0.78, 0.57–2.60; p = 0.12). Skin AF values ≥ 2.2 indicates presence of ED with a sensitivity of 70.0% and a specificity of 77.5%. Area under the curve was equal to 0.72 (95% CI: 0.60–0.85). Conclusions The presence of ED in individuals with T1DM is associated with HbA1c, the presence of at least one diabetic complication, and skin AF.

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Skin autofluorescence (a marker for advanced glycation end products) and erectile dysfunction in diabetes

Cited by 11 publications

References 38 publications

Glycation by glyoxal leads to profound changes in the behavior of dermal fibroblasts

Glycation by glyoxal leads to profound changes in the behavior of dermal fibroblasts

Advanced Glycation End Products: New Clinical and Molecular Perspectives

Erectile Dysfunction in Individuals with Type 1 Diabetes is Associated with Long-term Metabolic Control and Diabetic Complications: A Cross-Sectional Study

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