Skin and wound decontamination of multidrug‐resistant bacteria by cold atmospheric plasma coagulation

Daeschlein, Georg; Napp, Matthias; Lutze, Stine; Arnold, Andreas; Podewils, Sebastian von; Guembel, Denis; Jünger, Michael

doi:10.1111/ddg.12559

Cited by 76 publications

(76 citation statements)

References 28 publications

(40 reference statements)

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“…3). Different studies gave evidence of the successful decontamination of multidrug-resistant, contaminated wounds through nonthermal plasma (NTP) or cold atmospheric plasma (CAP) [88,89,90]. A secondary beneficial feature of plasma derives from its genomic effects [91].…”

Section: Plasmamentioning

confidence: 99%

Skin Wound Healing: An Update on the Current Knowledge and Concepts

Sorg

Tilkorn²,

Hager³

et al. 2016

Eur Surg Res

907

691

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Background: The integrity of healthy skin plays a crucial role in maintaining physiological homeostasis of the human body. The skin is the largest organ system of the body. As such, it plays pivotal roles in the protection against mechanical forces and infections, fluid imbalance, and thermal dysregulation. At the same time, it allows for flexibility to enable joint function in some areas of the body and more rigid fixation to hinder shifting of the palm or foot sole. Many instances lead to inadequate wound healing which necessitates medical intervention. Chronic conditions such as diabetes mellitus or peripheral vascular disease can lead to impaired wound healing. Acute trauma such as degloving or large-scale thermal injuries are followed by a loss of skin organ function rendering the organism vulnerable to infections, thermal dysregulation, and fluid loss. Methods: For this update article, we have reviewed the actual literature on skin wound healing purposes focusing on the main phases of wound healing, i.e., inflammation, proliferation, epithelialization, angiogenesis, remodeling, and scarring. Results: The reader will get briefed on new insights and up-to-date concepts in skin wound healing. The macrophage as a key player in the inflammatory phase will be highlighted. During the epithelialization process, we will present the different concepts of how the wound will get closed, e.g., leapfrogging, lamellipodial crawling, shuffling, and the stem cell niche. The neovascularization represents an essential component in wound healing due to its fundamental impact from the very beginning after skin injury until the end of the wound remodeling. Here, the distinct pattern of the neovascularization process and the special new functions of the pericyte will be underscored. At the end, this update will present 3 topics of high interest in skin wound healing issues, dealing with scarring, tissue engineering, and plasma application. Conclusion: Although wound healing mechanisms and specific cell functions in wound repair have been delineated in part, many underlying pathophysiological processes are still unknown. The purpose of the following update on skin wound healing is to focus on the different phases and to brief the reader on the current knowledge and new insights. Skin wound healing is a complex process, which is dependent on many cell types and mediators interacting in a highly sophisticated temporal sequence. Although some interactions during the healing process are crucial, redundancy is high and other cells or mediators can adopt functions or signaling without major complications.

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Section: Plasmamentioning

confidence: 99%

Skin Wound Healing: An Update on the Current Knowledge and Concepts

Sorg

Tilkorn²,

Hager³

et al. 2016

Eur Surg Res

907

691

View full text Add to dashboard Cite

show abstract

“…Atmospheric plasma at or near ambient temperature has led to a new field of plasma medicine [4,5], and cold atmospheric plasma (CAP) has attracted a lot of attentions due to its remarkable potential to affect biological processes [2,6]. In this context, the potential of CAP in diverse bio-medical applications has been explored, including disinfection, wound treatments, control of inflammation, blood coagulation, cancer therapy, and regenerative medicine [7,8,9,10,11]. The efficacy of CAP in the proposed applications relies on the synergistic action of the reactive oxygen species (ROS), reactive nitrogen species (RNS), free radicals, UV photons, charged particles, and electric fields [12,13,14,15].…”

Section: Introductionmentioning

confidence: 99%

A Novel Micro Cold Atmospheric Plasma Device for Glioblastoma Both In Vitro and In Vivo

Chen

Simonyan

Cheng

et al. 2017

Cancers

113

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Cold atmospheric plasma (CAP) treatment is a rapidly expanding and emerging technology for cancer treatment. Direct CAP jet irradiation is limited to the skin and it can also be invoked as a supplement therapy during surgery as it only causes cell death in the upper three to five cell layers. However, the current cannulas from which the plasma emanates are too large for intracranial applications. To enhance efficiency and expand the applicability of the CAP method for brain tumors and reduce the gas flow rate and size of the plasma jet, a novel micro-sized CAP device (µCAP) was developed and employed to target glioblastoma tumors in the murine brain. Various plasma diagnostic techniques were applied to evaluate the physics of helium µCAP such as electron density, discharge voltage, and optical emission spectroscopy (OES). The direct and indirect effects of µCAP on glioblastoma (U87MG-RedFluc) cancer cells were investigated in vitro. The results indicate that µCAP generates short- and long-lived species and radicals (i.e., hydroxyl radical (•OH), hydrogen peroxide (H2O2), and nitrite (NO2−), etc.) with increasing tumor cell death in a dose-dependent manner. Translation of these findings to an in vivo setting demonstrates that intracranial µCAP is effective at preventing glioblastoma tumor growth in the mouse brain. The µCAP device can be safely used in mice, resulting in suppression of tumor growth. These initial observations establish the µCAP device as a potentially useful ablative therapy tool in the treatment of glioblastoma.

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“…Although the exact mechanism of action is not fully understood, numerous in vitro studies have proven the high antibacterial efficiency of these systems (Daeschlein et al . , , , , Bender et al . , Matthes et al .…”

Section: Introductionmentioning

confidence: 99%

Comparison of polyhexanide, cold atmospheric plasma and saline in the treatment of canine bite wounds

Nolff

Winter

Reese

et al. 2018

J of Small Animal Practice

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Objectives To compare the efficacy of polyhexanide, cold argon plasma and saline in reducing bacterial bio‐burden in dog bite wounds. Materials and Methods Prospective blinded randomised clinical trial. Dogs were randomly assigned to one of the treatment groups by lottery and bacterial cultures obtained before and after treatment were compared. Bite wounds were surgically debrided and treated with polyhexanide, cold argon plasma or saline lavage. All wounds were cultured three times: directly after debridement, directly after prelavage with 2 mL/cm2 (saline in the saline and cold argon plasma group, or polyhexanide) and following the definitive lavage. Data were analysed using a generalised linear model for ordinal data. Results A total of 85 dogs were enrolled in this study (polyhexanide n=29, cold argon plasma n=28, saline n=28). Positive bacterial culture results after debridement were obtained in 53/85 (62.3%) wounds. Polyhexanide and saline lavage significantly reduced the bio‐burden, while cold argon plasma treatment did not. This effect was evident after prelavage when polyhexanide performed significantly better than saline and cold argon plasma as well as after final treatment. No significant differences were detected after prelavage or main treatment between saline and cold argon plasma. Clinical Significance Polyhexanide lavage achieved the best immediate and ultimate decontamination of bite wounds.

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Skin and wound decontamination of multidrug‐resistant bacteria by cold atmospheric plasma coagulation

Cited by 76 publications

References 28 publications

Skin Wound Healing: An Update on the Current Knowledge and Concepts

Skin Wound Healing: An Update on the Current Knowledge and Concepts

A Novel Micro Cold Atmospheric Plasma Device for Glioblastoma Both In Vitro and In Vivo

Comparison of polyhexanide, cold atmospheric plasma and saline in the treatment of canine bite wounds

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